In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.Vi föreslår att man gör följande saker:Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttetUtvecklar och implementerar den föreslagna klassificerarenGenomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering.

I denna uppsats behandlas frågan om äganderätt till mineraler. Uppsatsens syfte är att mot bakgrund av fastighetsbegreppet utreda hur synen på äganderätt till mineraler ser ut i Sverige och om senare förarbeten medfört att frågan blivit mer oklar. Metoden är baserad på den rättsdogmatiska metoden. Tillgången till doktrin och praxis har varit starkt begränsad och utredningen är därför främst baserad på förarbeten. I uppsatsen läggs särskild vikt på principuttalanden från lagstiftaren.

Formulation and testing of a new type of chainsaw lubricantThe company Agro Oil has come up with the idea of developing an environmentally adapted chainsaw lubricant with a new unusual base fluid. A field test with a tree-harvester shows unsatisfactory low temperature properties and high wear. In this work the viscosity, price and pour point of different combinations of the given base fluid and three other base fluids have been investigated. Desired viscosity and price are determined for different formulations. The new lubricants tested show lower pour points than almost all products on the Swedish market.A test equipment has been developed to investigate the wear.

This study has taken place in two nature reserves in the municipality of Uppvidinge in the Kronoberg region in southern Sweden. These have been chosen by the County Administrative Board to be part of a bigger landscape of fire affected nature, and prescribed fire has been implemented in these. The areas are called Ösjöbol and Berga fly and have been burned 2012 and 2013 respectively. Non-burned areas have been inventoried as well. Vascular plants and mineral soil have been inventoried with the Hult-Sernander-Du Rietz scale of five levels. Issues emanate from the County Administrative Board?s goals for prescribed fire: how sticks and herbs were affected, how much soil was uncovered, how the structure and fire favoured species were affected, and how deciduous trees and spruces (Picea abies) were affected. The study shows that the soil increased, as well as the fire favoured species.

During whole-tree harvesting, there is a risk that the soil will become impoverished of nutrients. To prevent this, nutrients are returned to the harvested site as wood ash. This action also reduces the risk of soil acidification. Nevertheless, the ash does not result in any increase of tree growth if it is added on mineral soils, which is where the measure is most commonly used in Sweden. Due to the lack of growth response, the interest from landowners is small, which is why ash amendment today is not done enough to compensate for the need.

Phosphorus is essential to all plants and is taken up from the soil as the ion H2PO4 - (and to some extent HPO42-). Plant availability depends on, among other factors, the soil pH. In order to provide for plant available phosphorus, it is applied as mineral fertilizers and farmyard manure. However, that causes phosphorus leaching from arable land to surrounding surface waters and lakes and contributes to the eutrofication. There are three types of soils in Sweden that are at higher risk of contributing to phosphorus leakage.

Sammanfattning Kisel (Si) är det näst vanligaste ämnet (drygt 25 %) i berggrunden. Bara en del av detta är tillgängligt som monokiselsyra, den form i vilken kisel är upptagbart för växter. Jordens kiselinnehåll är beroende av bergets vittring. Mycket höga halter av kisel återfinns i naturen i vulkaniska substrat (pimpsten innehåller drygt 66 % Si och scoria närmare 47 % Si), i diatomit (ett mineral bestående av resterna från encelliga kiselhaltiga alger, s.k. diatoméer) innehållande 66 % Si, scoria approximatly 47% Si), in diatomite (a mineral consisting of the remainings of single celled algae, diatoms containing < 40 % Si) and in some clays (30-60% Si).

To save energy it?s popular to build houses with low energy loss. In these houses the walls are keeping a lower temperature in the external side which leads to a higher relative humidity. In addition more material, such as wood studs, are used which means that there is more moisture to dry. More moisture in combination with higher relative humidity may pose a higher risk of microbial growth.

Testosterone is a steroid and anabolic hormone found in all mammals. Previous research indicates that testosterone levels correlates with physical capacities related to physical performance. However, these studies refers only to men and boys. The aim of present study was therefore to investigate the potential relationship between body composition, strength, power and endurance capabilities in relation to blood levels of testosterone and DHEAS in young female football players. Seventeen female elite football (age: 15,4 ± 0,6, body mass: 57,2 ± 7,4kg, height 1,65 ±0,04m) players volunteered for the study.

Detta examensarbete utfördes på Metso Minerals (Sala) AB i Sala mellan mars och augusti 2006 och handlar om kartläggning av ledtiden vid produktlinje pump. Förutom kartläggningen har även analys och förslag till förbättring arbetats fram. Denna rapport är resultatet av detta examensarbete vilket har skrivits som avslutning på programmet Ergonomisk design& produktion med inriktning Industriell produktion vid Luleå Tekniska Universitet. Metso Minerals (Sala) AB har cirka 200 anställda och är en del av affärsområdet Minerals. Verksamheten i Sala tillverkar slurrypumpar samt processmaskiner och är en stor leverantör av pumpar till mineral och processindustrin.

This study was made on former agricultural areas that had been planted with spruce or pine, in the county of Västerbotten. The goal of the project was to investigate suspected deficiency of boron in these areas. Several objects have been reported for possible deficiency and some of these have the characteristic symptoms of boron-deficiency. The hypotheses are that 1) Boron deficiency is present at several of the investigated objects. 2) Planting of forest on cultivated postglacial sediments leads to a successive podzolisation with a decreasing content of organic matter in the mineral soil, due to decomposition, and a decreasing pH in the soil, even on locations that have been added lime. To be able to confirm or reject the hypotheses a number of tests were performed.

Osteoporos är en sjukdom som under de senaste decennierna ökat. I Sverige inträffar det cirka 70 000 osteoporosrelaterade benbrott varje år. Den största gruppen som drabbas är kvinnor efter menopaus. Syftet var att belysa faktorer som är av betydelse för prevention av osteoporos och osteoporosrelaterade frakturer hos postmenopausala kvinnor. Metoden var en systematisk litteraturstudie bestående av 10 vetenskapliga artiklar varav 9 baserade på kvantitativ och en på kvalitativ forskning.

The purpose of this thesis is to investigate if vacuum insulation panels are a competitive alternative to traditional insulation.Vacuum insulation has been used as insulation in buildings in Germany and Switzerland for a few years. In Sweden the high-performance insulation material is mainly used in refrigerators, freezers and vacuum thermoses. The development is proceeding well and vacuum insulation is becoming more common in the construction industry. Since it is a relatively unexplored material on the market there are many flaws and problems with the material.The questions we have chosen to answer in this report include an increased knowledge of vacuum insulation, various problems that may arise when handling/installation and the pros and cons of vacuum insulation compared to standard traditional insulation.The methods we have chosen to use in this report is a literature study, interviews with various suppliers and manufactures and calculations to show the differences between traditional and vacuum insulation. Vacuum insulation is 5-10 times better insulation than standard traditional mineral wool insulation, mainly at the beginning of its lifetime.Vacuum insulation panels (VIP) have a service life of approximately 50 years, whereas traditional insulation has a service life of approximately 100 years.

In a column experiment the effect of three different biochars on nitrous gas emissions, nitrogenretention and water filled pore space (WFPS) were studied in a Swedish sandy soil, classified as aCryopsamment. Ammonium retention was positively correlated, while KCl-extractable ammoniumwas negatively correlated, with the specific area of the biochars. The question was raised whether thenegative correlation between extractable ammonium and biochar specific area reflects the amount ofammonium present in the soil or its extractability from the biochar, and whether methods forextracting soil mineral nitrogen, like KCl-extractions, have to be modified for application on biocharamended soils. There was a tendency of increased nitrate retention and KCl-extractable nitrate withbiochar and KCl-extractable nitrate was positively correlated with the specific area of the biochar. pHwas higher for all biochars compared with control, although the differences were not significant, andWFPS was lowered by biochar addition, significantly in one case.

Bovine milk is an excellent source of bioactive peptides. The bioactive peptides are in an inactive state in the protein and are released during enzymatic activity, e.g. during cheese ripening. Specific properties of bioactive peptides from ripened cheese has been reported, e.g. antihypertensive, antioxidative, antimicrobial, immunomodulatory and mineral binding.