Sökresultat:
82 Uppsatser om Molecular inversion probe - Sida 4 av 6
Validering av realtids-PCR-metod för Herpes simplex- och Varicella-zoster virus
I detta arbete studeras hur vinterklimatet i Sverige kommer att skilja sig åt mellan perioderna 2071-2100 och 1971-2000, med avseende på parametrarna temperatur, nederbörd, vind, snödjup och snöutbredning. Klimatmodelldata (CORDEX) i upplösningen 12.5 km baserade på tre olika strålningsdrivningsscenarier (RCP-scenarier) behandlades i Matlab och modellerades över Sverige. För att analysera upplösningens betydelse tillhandahölls även data i upplösningen 50 km för en tioårsperiod. Scenariot RCP 8.5 kan ge så mycket som 10 grader varmare vintrar, med uppemot 50% mer nederbörd. För RCP 2.6 förändras nederbörden med -5 till 20%, och temperaturen ökar med 1-4 grader.
Ett nytt multiplext PCR-protokoll för identifiering och detektion av Shigella och enteroinvasiv E. coli (EIEC) från livsmedel
This report is the result of a project in the course Independent Projekt in Molecular Biotechnology at Uppsala University during the spring of 2014. The foremost purpose of the course is to give students the opportunity to carry through exstensive work in a project environment. This project was formed based on a comission from the biotechnology company SweTree Technologies, and the goal has been to compose a summary of the different techniques and methods that exist in the field of mass propagation of trees through the method of somatic embryogenesis.The project group has obtained information about the area mainly throgh reading patents, trying to find key components and bottlenecks in other companies? somatic embryogenesis technologies. This paper is divided into different sections, containing the patents of the automation of different steps in the process.
Optimization of immunoassay parameters in multiplex in the high throughput protein detection technique Proximity Extension Assay
The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.
Diagnostiska metoder för växtvirus i nematodvektorer :
ABSTRACT
Tobacco Rattle Virus (TRV) is a soilborne plant virus. It is an important pathogen in potatoes where it causes spraing disease. This disease can lead to significant economical loss for the farmer, so it is imperative to get the proper diagnose before planting.
TRV is spread naturally by free-living nematodes (Trichodorus and Paratrichodorus). Transference of TRV to a plant occurs when the virus-carrying nematodes feed on plant roots. The relationship between virus, nematodes and plants is complicated, and has to these days not been fully characterized.
The diagnostic methods available today do not work sufficiently.
Utveckling av hygienfunktioner i diskmaskiner : Desinfektion med hjälp av UV-ljus och ozon
This thesis within the field of Integrated Product development at the Royal Insitute ofTechnology, performed at Primary Development Dishcare at AB Electrolux, is supposed to finda solution to enhance the hygiene inside an Electrolux dishwasher. The outcomes of this thesisare functional prototypes to evaluate the efficiency of disinfection by the use of Ultra VioletLight and Ozone.The thesis has followed Electrolux Primary Development process, with delivarables andcheckpoints.Ultra violett light with a wavelength of around 264 nm penetrates through the cell wall ofbacteria and micro organisms and causes a molecular rearrangement of the cells DNA. Thisprevent the bacteria from reproducing and it is considered dead. Commonly used UV-C lampstransmits the wavelength 254 nm which is very close to the optimum for germicidal action.A competitive brand have reacently launched a dishwasher claiming to achieve greaterdisinfection with an UV feature. This dishwasher was tested during the thesis and proven tohave a very low effect on the hygienic result.Two prototypes were devolped and tested for UV disinfection of dishware.
Kvantfysikens grunder i Gy2011 : En tolkning av kursplaner för fysik 1 och 2
AbstractThe beginning of the 20th century gave birth to quantum physics. Within years it revolutionized material science and converted the theoretical foundations of modern chemistry, and to some extent even biology, to physics? new branch, molecular physics. Merger of relativistic and quantum physics soon led to the development of quantum field theory and a deeper understanding of the elementary constituents of matter. Part of this development found its way into high school textbooks as early as in the 1950s.
Examination of mycorrhizal associations of Allanblackia stuhlmannii : a tree under current domestication
Allanblackia is a tree genus native to humid forests in West, Central and East Africa. Its fruits contain large seeds from which edible high-quality fat can be extracted. In order to create a sustainable supply chain of Allanblackia seeds, a domestication program has been initiated with the purpose to enable smallholder farmers to grow the tree as an agroforestry component and cash crop. More knowledge is required to understand the tree?s biology and possible symbioses involving soil microorganisms with plant growth enhancing poten-tial.
Avelsarbete med mjölkproducerande getter ? fokus på Norge och Frankrike
The main part of the goats in the world is located in developing countries, whereas active breeding programs are mainly restricted to Northern America and Europe. Goat milk can be processed to several products and goat cheese is the main product. An organized breeding program is carried out in France and Norway but is not present in Sweden. Important selection traits for milk producing goats include milk, protein and fat yield along with protein and fat content. Functional traits have been more important the past years and they have large economic importance for the farmers.
Utsläppsnivåer från småskalig biobränsleanvändning
Biobränsle är en förnyelsebar energikälla som har stor potential för enskilduppvärmning av villor. Men en stor del av de biobränslepannor, som används idag, är inte miljögodkända samt saknar ackumulatortank, vilket kan orsaka storautsläpp av hälsofarliga ämnen.Syftet med det här examensarbetet är att undersöka hur utsläppsnivåerna frånsmåskalig biobränsleanvändning skulle förändras, om man bytte ut dagens uppvärmningssystemmot bästa biobränsleteknik.Studien har utförts i villaområden i kommunerna Skellefteå, Lycksele ochStoruman. Utsläpp från dagens biobränsleanvändning har jämförts med hurutsläppen skulle se ut om alla hushåll bytte till bästa teknik. Med hjälp avinventering av bland annat energibehov och använd teknik, samt definiering avbästa teknik har beräkningar och datasimuleringar utförts. Simuleringarna hargjorts i spridningsprogrammet Airviro, där samkörning av klimat-, energianvändnings-och emissionsdata ger resultat i emissionshalter i luft.
Lätta bromsok
SAAB Automobile ABis a car manufacturer within General Motors corporate group. SAABAutomobile ABhas a big part of the responsibility for the brake development in the corporategroup. The car industry is facing harder environmental requirements, which influence the brakedevelopment. The focus is to develop brakes with low drag and sustained brake feeling andperformance. Important characters for the brake caliper are high stiffness and low weight, highstiffness results in high performance and low weight is important because it is unsprung mass.This thesis investigates the possibility to improve the performance of the brake caliper for aGMproject.
Feedback och hälsa : en studie hos ett statligt verk
Today, many discussions are taking place through different platforms concerning the huge problem of ill health within modern society. Ill health tends to affect both children and adults. For the adult sector ill health may lead to doctor?s notification of illness in the short term and on the long term permanent disability benefit. In the mass media there are often articles published that point out the rising number of people with sick pay and people being given permanent disability benefit.
Bestämning av utfallet av translokationen t(11;18)(q21;q21) hos patienter med MALT-lymfom genom FISH analys
Lymphoma is a group of malignant tumour diseases developing in the secondary lymphatic system. These diseases can develop in all organs as lymphocytes are ubiquitously in the body. In connection to mucus membranes we find mucosa-associated lymphoid tissue, MALT, in which lymphoma can spontaneously but slowly develop, mostly at chronic inflammation or at autoimmune diseases. Today these diseases are incureable with the exception of some cases caused by Helicobacter pylori-infection. Antibiotic treatment of these cases can induce remissions.MALT-lymphomas have characteristic histological and molecular properties.
Common misconceptions about everyday astronomy-related phenomena among students in the 9th grade
Students of all ages host a wide variety of scientifically inaccurate ideas and conceptions about everyday astronomical phenomena, such as the seasons, the moon phases, and gravity. The field of Astronomy Education Research has over the last decade experienced an accelerating growth, although the majority of studies have been conducted in the USA. In this work, the 9th grade students of a typical Swedish school were surveyed by means of a questionnaire in order to probe their conceptual understanding of several key concepts in astronomy. In the end, the number of respondents amassed to a total of 90. The results were analyzed with a constructivist approach in light of conceptual change theory and phenomenological primitives.
Användargenererad data i tjänstedesignprocessen
Syftet med studien är att ta reda på hur tre olika innovativa tekniker kan användas för att samla in användarinsikter i en tjänstedesignprocess. Detta genomförs med ett designcase som handlar om besökarnas upplevelse av Norrköpings Symfoniorkesters konserter. Symfoniorkestern behöver hjälp med att locka fler besökare till konserterna och nya sätt behöver användas för att samla in användarinsikter för att få så många olika perspektiv som möjligt av konsertupplevelsen.Det var 20 personer som deltog i studien med en blandning av personer, dels de som idag går på Norrköpings Symfoniorkesters konserter och dels de som inte besöker dem. Deltagarnas uppgift i studien var att gå på två av deras konserter och dokumentera sina upplevelser med hjälp av tre olika innovativa tekniker: designsond, app och automatisk kamera.Resultatet av studien visar att den första tekniken designsonden gav en fördjupad bild av deltagarnas syn på konsertupplevelsen och hjälpte till att bredda fokus i tjänstedesignprocessen. Den andra tekniken,appen, kunde ge en mer detaljerad information i realtid om deltagarnas konsertupplevelse på plats.
Återfjädring vid bockning av höghållfast stål
Svenska Aerogel AB has developed the material Quartzene® which is of the typeprecipitated silica and contains calcium - magnesium silicate ((Ca, Mg) SiO3). Thematerial is used for molecular filtration of contaminated air. Qartzene?, which is inpellet form, is porous and has a high internal surface which is a requirement for themolecular filtration. With the use of various impregnations, filtration can affect thedesired outcome.