Nematode-transmitted plant viruses (such as nepoviruses) cause great economically losses in commercially important plants such as tomatoes, grapevines and raspberries all over the world. To avoid spread of the viruses the interest in mapping the viruses has increased. One of the important nepoviruses infecting European red raspberries (Rubus idaeus) is Tomato black ring virus (TBRV). TBRV has earlier been confirmed in raspberry samples from Belarus by ELISA and the aim of this bachelor degree project was to confirm these results by amplifying, cloning and sequencing the coat protein (CP) gene of the virus. In total eight viral cDNA samples, from two different raspberry samples, were amplified and sequenced.

The development of IT systems is usually accomplished by some form of system development methodology. It can be performed as the waterfall method, where each phase is completed before the next begins. One of the reasons to follow a development methodology is that the process is more structured, faster and that the product will have higher quality. One risk of not using a system development approach is that the code can be unstructured and difficult to maintain.This paper describes an alternative method in which the development occurred without the use of any system development methodology. This has been possible because there was a clear goal of the program that should be developed.

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

Syfte: Att med hjälp av videoanalys och programvaran Dartfish, undersöka eventuella skillnader i stödfasen på vinkeln mellan calcaneus och underlaget, hos personer med Pes planus och pes cavus, både efter en- och 45 minuters löpning på löpband.  Studiedesign: En experimentell studie. Metod: Trettiofyra personer vars fot/fötter uppmättes till pes cavus och pes planus enligt kriterierna för Medial Longitudinale Arch -angle, inkluderades i studien. Mätningarna gjordes på en stillbild med digital goniometer i programvaran Dartfish. Därefter mättes calcaneus vertikala mittlinje i förhållande till underlaget i stödfasen både efter 1 minut och 45 minuter, när försökspersonerna sprang barfota på ett löpband.

This master?s thesis deals with integrating simulations using Agilents Electronic Design Automation tool ADS with customized Matlab scripts, for solving complex analog and digital radio architectures. In particular, it addresses predistortion, realized in the digital domain, of power amplifiers, modeled in the analog domain. The former is implemented in Matlab while the latter is implemented in ADS. Two versions of integrating the two systems have been tested: 1) Iterative approach on sample basis and 2) Scheduled batch solution by matrix inversion.

The focus of this study is on the nature of social support from the rape survivor's perspective. Research has shown that social support is essential to the survivor's ability to recover from rape and that it acts as a powerful tool for the survivor to effectively deal with the PTSD symptoms that follow rape. The research also shows that survivors often receive or interpret reactions after a rape as negative. Hence, these reactions can be detrimental tothe healing process.Qualitative interviews with six women on their experience of social support after rape form the base of this study and Anthony Giddens terms ontological security, fateful moments and bodily self-management are a part of the analytical apparatus. Rape is commonly analyzed from a feminist perspective.

Malignant melanoma represents 4-7% of all canine neoplasms and they are the most common malignant tumors of the oral cavity and digits. These tumors are often aggressive and metastasize quickly. To date there is no effective treatment and survival times are short. Most dogs are euthanized due to complications caused by metastases. Melanin pigment is a hallmark of melanomas and is usually present in large amounts in these tumors.

Syftet med denna uppsats är att undersöka vilka morfologiska och syntaktiska strukturer i det svenska språket som ställer till problem för de som lär sig svenska som ett andra språk. Den undersöker också om inlärningsgångarna ser lika ut för inlärare med olika modersmål, eller om det går att se någon påverkan från språket som inläraren har med sig i bagaget. Metoden som används är samtal tillsammans med fem informanter som studerar svenska på en gymnasieskola i Sverige. Två informanter har arabiska som modersmål och tre informanter har thailändska som modersmål. I dessa samtal har både antalet rätta och felaktiga morfologiska och syntaktiska strukturer räknats.

Beställningsfrekvensen för detektion av Trichomonas vaginalis samt Mycoplasma genitalium i Jönköpings län är låg jämfört med den för Chlamydia trachomatis och Neisseria gonorrhoeae.  Både T. vaginalis och M. genitalium har associerats med infektion av humant papillomvirus (HPV) samt kan bland annat orsaka salpingit med infertilitet som potentiell komplikation. Patogenerna har även beskrivits öka risken för transmission av HIV. Syftet med studien var att detektera T.

The focus of this study is on the nature of social support from the rape survivor's perspective. Research has shown that social support is essential to the survivor's ability to recover from rape and that it acts as a powerful tool for the survivor to effectively deal with the PTSD symptoms that follow rape. The research also shows that survivors often receive or interpret reactions after a rape as negative. Hence, these reactions can be detrimental tothe healing process.Qualitative interviews with six women on their experience of social support after rape form the base of this study and Anthony Giddens terms ontological security, fateful moments and bodily self-management are a part of the analytical apparatus. Rape is commonly analyzed from a feminist perspective.

Forestry is an important industry in Sweden, with clear-cut as the most commonly used method for harvesting. Here the long-term impact of clear-cut on lake water quality (dissolved organic carbon (DOC), nitrogen, phosphorus and pH) has been investigated, based on 23 lakes sampled during autumn. Data for additional 15 lakes sampled for the Swedish monitoring program were also used. Lakes were classified as 5, 25, 40 and 60 year based on data of the forest age in the catchment, gathered from the Swedish national forest inventory. Clear-cut lakes were classified as 5 years (>20% of the catchment clear-cut during the last 10 years).

African swine fever, ASF, was first described 1921, in Kenya. The virus has thereafter spread through many African countries as well as in Europe, Asia, South- and Central America. The virus is spread via three different cycles, of which one is sylvatic and includes warthogs and soft ticks. The other two are between domestic pigs and the one most common one is caused by humans, who not always realize the seriousness of precautions. The infection manifests as hemorrhagic fever, but can also be more or less unnoticed. The aim of this study was to use molecular tools to detect and partly genetically characterize ASFV both in soft ticks and in serum from domestic pigs in Uganda. Comparing viruses detected in ticks with those detected in domestic pigs I also wanted to investigate possible links between the sylvatic and domestic cycle.

This report is the result of a project in the course Independent Projekt in Molecular Biotechnology at Uppsala University during the spring of 2014. The foremost purpose of the course is to give students the opportunity to carry through exstensive work in a project environment. This project was formed based on a comission from the biotechnology company SweTree Technologies, and the goal has been to compose a summary of the different techniques and methods that exist in the field of mass propagation of trees through the method of somatic embryogenesis.The project group has obtained information about the area mainly throgh reading patents, trying to find key components and bottlenecks in other companies? somatic embryogenesis technologies. This paper is divided into different sections, containing the patents of the automation of different steps in the process.

This report is the result of a project in the course Independent Projekt in Molecular Biotechnology at Uppsala University during the spring of 2014. The foremost purpose of the course is to give students the opportunity to carry through exstensive work in a project environment. This project was formed based on a comission from the biotechnology company SweTree Technologies, and the goal has been to compose a summary of the different techniques and methods that exist in the field of mass propagation of trees through the method of somatic embryogenesis.The project group has obtained information about the area mainly throgh reading patents, trying to find key components and bottlenecks in other companies? somatic embryogenesis technologies. This paper is divided into different sections, containing the patents of the automation of different steps in the process.

There are two species of potato cyst nematode (PCN), the yellow, Globodera rostochiensis, and the white, Globodera pallida. Each of them can appear in different pathotypes. These are separated by testing the nematode ability to multiply on a number of potato cultivars with different resistance properties. The aim of this project was to examine the virulence of Swedish and foreign PCN populations and to compare two test methods. Biotests are today the only available tests used to study virulence.