In this thesis, three separate experiments have been performed on different aspects of the interaction between the causal agent of clubroot, Plasmodiophora brassicae, and its hosts. In the first experiment the pathotype of the P. brassicae single spore isolate, which is currently used to construct a reference genome, has been classified using the ECD bioassay. The disease severity of infected plants was scored according to two different scales and the pathotype was determined according to three previously published guidelines. The results were compared to previous published studies describing the e3 isolate. The life cycle of P. brassicae is not well understood.

Land consolidation is a major problem in many countries in Europe. In Sweden, mainly the state of Dalarna, there is a strong consolidation of forestland, probably the strongest in all of Europe. The consolidation is a big obstacle for forest management and ownership questions due to the often long and narrow strips of land. One of the main reasons for land consolidation in Dalarna results from the heritage procedure, were land is divided between all of the inheritors. To improve the consolidation large surveys in the consolidated villages in Dalarna are carried out. Many laws have to be followed due to the survey.

This is a literature study in which I investigate if there are scientific studies that form the basis of the Swedish dog world views concerning the spaying of bitches. The positive impacts are said to be reduced problems with pseudopregnancy, reduced risk of pyometra, reduced risk of mammary and uterine tumors, an earlier cease of bone growth, a more active and happier dog and a bitch who is more tolerant of other dogs. The negative consequences are said to be urinary incontinence, increased aggressiveness, lower metabolism and the increased risk of obesity, a more lethargic individual and altered coat.There are studies indicating that spaying reduces behavioral problems associated with heat and pseudopregnancy. Assuming no ovary tissue persists after the procedure, and there is no progesterone production, the risk of pyometra decreases. Early spaying, before the first heat, reduces the risk of mammary tumors.

The purpose of this study was to examine the extent to which incontinence aids, used in local authority/municipal nursing homes, were adapted to the resident?s urine-leakage volume and to find out how nurses perceived the current situation concerning individual testing of incontinence aids in municipal nursing homes. The study method was a quantitative empirical study and was carried out in two phases. The first phase was a weighing test, carried out in three nursing homes, whereby the incontinence aids used by 25 residents during a 48 hour period were weighed. The second phase was the completion of a questionnaire by the municipal nurses working in the same local authority.

Background: Isolation care may be necessary when a patient is carrying infection or is being inflicted with disease that causes deficit immune system. Being isolated means that the patient is in a confined space and may stay only there. Even in ancient times, people discovered the importance of isolating people carrying an infection from the rest of society. According to the Infectious Diseases Act, patients should be isolated in order to reduce the spread of infection both from the patient to the surroundings as well as the opposite. Isolation care can be psychologically stressful for some patients.

Bovine viral diarrhoea virus (BVDV) is the causative agent of a complex of disease syndromes in cattle with high economical and welfare impacts. BVDV occurs as two biotypes; cytopathic (BVDVcp) and noncytopathic (BVDVncp) determined by differential effects on cultured cells and can also be divided into two genotypes (BVDV1 and BVDV2) on the basis of genomic diversity. The interaction between BVDV and the host?s immune system is regarded a key aspect in the sequel of BVDV infection. Infection with BVDV normally causes an acute transient infection, with mild to subclinical signs, but occasionally results in severe and even fatal disease.

The Bovine respiratory syncytial virus (BRSV) causes bronchiolitis and interstitial pneumonia, predominantly in calves, and is a major cause of bovine respiratory disease worldwide. In humans, BRSV is paralleled by the closely related Human respiratory syncytial virus (HRSV), an important cause of respiratory disease, most severe in infants.The clinical signs and pathology during RSV infection is caused, not only by the direct effects of viral replication, but also by the response of the host immune system. The immunopathology of RSV has long obfuscated our understanding of the disease, and development of effective treatment and vaccines will be very difficult until greater knowledge is gained.One of the components of the immune system that has come into focus in RSV research the last few years, is the Toll-like receptor 4 (TLR4). The TLR4 receptor is well known as the receptor that binds lipopolysaccaride (LPS), and initiates the host response to bacterial infection. Recently, it has been shown that the fusion protein of RSV also interacts with, and up-regulates the expression of, the TLR4 receptor.

IBS patients experiences of eating the low FODMAP-dietIrritable bowel syndrome (IBS) is a common, functional gastrointestinal tract disorder. The disease is benign but may cause much suffering for the individual. The treatments available are used to relieve symptoms. One nutrition therapy is to exclude fermentable oligo-, di-, monosaccharide and polyols, so callde FODMAPS, from the diet. This diet is proven successful and even significantly better than the standard nutrition advice for IBS.

The Bovine respiratory syncytial virus (BRSV) causes bronchiolitis and interstitial pneumonia, predominantly in calves, and is a major cause of bovine respiratory disease worldwide. In humans, BRSV is paralleled by the closely related Human respiratory syncytial virus (HRSV), an important cause of respiratory disease, most severe in infants.The clinical signs and pathology during RSV infection is caused, not only by the direct effects of viral replication, but also by the response of the host immune system. The immunopathology of RSV has long obfuscated our understanding of the disease, and development of effective treatment and vaccines will be very difficult until greater knowledge is gained.One of the components of the immune system that has come into focus in RSV research the last few years, is the Toll-like receptor 4 (TLR4). The TLR4 receptor is well known as the receptor that binds lipopolysaccaride (LPS), and initiates the host response to bacterial infection. Recently, it has been shown that the fusion protein of RSV also interacts with, and up-regulates the expression of, the TLR4 receptor.

Influenza is a viral infection that affects global health and economy with its endemic and sometimes pandemic spread. Rapid detection of Influenza viruses enables antiviral use and can bring financial savings. It is also essential for the global surveillance of prevalent Influenza strains. RT-PCR is considered the most specific and sensitive method for detection of Influenza, but Influenza mutates at a high rate and it is therefore crucial that RT-PCR methods are updated regularly.In 2014, Cepheid released their Xpert Flu/RSV XC assay, which can detect Influenza A and B and RSV by multiplex RT-PCR in approximately one hour. The aim of this study was to evaluate this assay at Laboratoriemedicin Västernorrland by using the laboratory?s previous PCR assay for detection of Influenza viruses as reference method.Real-time RT-PCR was used to compare Xpert Flu/RSV XC to the reference method.

HIV/AIDS is a chronic disease that has increased and will continue to increase. Most health care workers will one way or another come into contact with it. The purpose of this literature review was to describe factors contributing to nurses' attitudes towards patients with HIV/AIDS. Sixteen articles were analyzed and quality assessed. The results revealed gaps in knowledge among nurses.

ABSTRACTMultiple sclerosis (MS) is the leading cause of adult disability inSweden. Hematopoietic stem cell transplantation (HSCT) can be used as a treatment option for patients with MS where the disease has an aggressive course. The treatment is preceded by high-dose chemotherapy which lowers the patient's immune system. It is therefore of interest to study infectious complications associated with HSCT in this patient.Aim: The purpose of this study was to identify infection problems in this population associated with HSCT, and to study differences in infectious complications between this group and patients diagnosed with lymphoma who have undergone the same treatment.Method: The study was a retrospective descriptive study with quantitative data. A medical record review was conducted.

The purpose of this thesis has been to signify what creates quality for the dwellers. Bycreating a definition of the term we have showed how well the qualitycriteria?s has been fulfilled in the newly built one family house area Pumpkällehagen in Viskafors.The questions the thesis gives answers to is: What is quality for the dwellers inhousebuilding of today? and How well have these quality aspects been carried out in tracthousing areas? These questions have taken us to our main goal: To describe what qualityfor the dwellers is and how well it has been carried out in a certain area.The literature review is describing what was published before the making of this thesis.From four different publications within the concept of quality an analysis template hasbeen enacted. This template has been used for our case study, but is also supposed towork in future analysis of tract housing areas.Interviews was carried out with the areas architects; Ola Nylander and Mattias Karlsson,as well as the client; Mikael Bengtsson. From these we have gathered their point of viewon the background of the area, as well as their view on the term quality for the dwellers.Our work has brought us to enact nine different quality aspects, which we considercreates quality for the dwellers:? The Non-measurable properties? The rooms relative connections? Sufficient and user-friendly property complements? The property complements connection to the apartment? Location of the apartment and connections to the environment? Opportunities of outdoor activities? Orientation and transportation within the area? The social unity? The cost of the apartment and its form of tenureFrom the case study, using the analysis template, we can state that Pumpkällehagen is avery successfully built area where most of our assessment criteria?s and quality aspectshave been fulfilled.

Weaning is one of the most critical events in the piglet production with problems such as growth check and post-weaning diarrhea as a result. The lighter the piglets are when the growth check occurs, the harder they have to get back to normal growth rate. Creep feeding of piglets is used to reduce the problems by increasing the weaning weight and adapting the digestive tract to a starter diet. Hodge (1974) showed that the sow?s milk can not provide enough nutrients for maximal growth rate.

The purpose of this study was to develop a reliable molecular-genetic method to determine the different species of helicobacter in dogs. The study is part of a larger project to map the prevalence of Helicobacter spp in healthy and sick dogs in Sweden, and to determine the possible connection of Helicobacter spp infection with gastrointestinal diseases in dogs. Several published studies have reported on the prevalence of Helicobacter spp in dogs. The problem is that three of the most common species are so alike that a 16SrRNA-PCR with sequencing is not able to differentiate between them. In this study, DNA has been purified from samples and then a Multiplex PCR has been performed. Multiplex PCRs use multiple primers in one single PCR.