Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

FLUTD - Feline Lower Urinary Tract Disease ? is a complex disease and a common sight in the veterinary setting. Although FLUTD excluding urethral obstruction may be discomforting for the cat and its owner due to symptoms such as periuria, stranguria, hematuria, dysuria and pollakiuria, obstruction presents an acute and potentially lethal form of the disease as a result of uremia and disturbances in fluids, electrolytes and acid-base balances. A study of literature has been performed to investigate the causes of FLUTD with urethral obstruction and the actions available to the owner to avert them. Potential causes of obstructive and non-obstructive FLUTD have been found to include infections, uroliths, urethral plugs, neoplasia, anatomic abnormalities, trauma, neurogenic or iatrogenic causes. Most commonly a cause cannot be identified and the terms idiopathic FLUTD or feline idiopathic cystitis are used. As the mortality rate due to urethral obstruction likely is somewhere around 5.8-8.9% and FLUTD including and excluding urethral obstruction constitute great inconveniences, precautionary measures are warranted.

Bovine Respiratory Syncytial Virus (BRSV) is a common cause of respiratory disease among young cattle. The virus causes severe losses; the herd mortality rate can sometimes be as high as 20 %. In this study the avidity (i.e the antigen binding force) of BRSV specific antibodies was measured to see if there was a difference between antibodies produced during an acute phase of infection and antibodies produced by earlier infected animals. A commercially available ELISA-testkit against BRSV-specific antibodies was used and an incubation step with 6M urea was added. The effect of the urea is that it breaks the weak bonds between antibodies and antigen while the stronger bonds remain intact. Four different groups of animals were included in this study; seven calves that were naturally infected, three acutely infected calves with known time of infection, five cows that were seropositive during several years and four experimentally infected calves that had been a part of a vaccine trial. The results of this study showed that antibodies produced during the acute phase of an infection had a low avidity and that the avidity increased with time after infection.

Every year begomovirus infections cause severe constraints to the tomato production in Nicaragua. Extensive yield losses are followed by large economical losses, both for the country and the single farmer. During recent years the use of begomovirus resistant or tolerant tomato cultivars has increased in Nicaragua as an effort to improve yields. Still, begomovirus infection is a large threat to the tomato production. Therefore, ongoing work in Nicaragua is focusing on finding new, better, begomovirus tolerant or resistant tomato cultivars.

Background Nightingale attention that hygiene was an important task to prevent health- related infection. Today there are guidelines how hand hygiene should be followed, to prevent health- related infection. Good knowledge and education in hand hygiene and its consequences if it?s not followed is of great importance. Hand hygiene should be performed before and after the clean and the unclean work with patients and materials.

Introduction Postoperative wound infections leads to increased patient suffering and cost society money because it leads to longer hospital stays. Previous research shows that patient related risk factors such as smoking, diabetes, Chronic Obstructive Pulmonary Disease (COPD), advanced age (>65 years) a low preoperative hemoglobin level (<140g/L) and a high BMI (> 30) increases the risk of suffering a postoperative wound infection. Aim To investigate whether patients that underwent surgery with Coronary artery Bypass Graft (CABG) in a university hospital in central Sweden in 2013 and had any of these risk factors more often suffer from post-operative wound infection.MethodThe study has a descriptive design and is based on an ongoing infection registration area of operations. The study included 148 patients out of 255 (58%) undergoing CABG in 2013. Possible risk factors were collected through medical record review.Results There was no significant correlation between surgical wound infection and patient-related risk factors such as smoking (not quit smoking four weeks before surgery), diabetes, COPD, advanced age (>65 years), low preoperative hemoglobin (<140g/L) or a high BMI (>30) and suffered a postoperative wound infection.Conclusion More research and a larger sample is needed in the field to produce a reliable result could be presented..

The flagellate Giardia intestinalis has been known to cause diarrhea in both man and dog. Results from epidemiological studies have indicated that transmission of the parasite from dog to man is plausible even though that route of infection has yet to be shown. Several international studies have shown that the parasite frequently occurs in asymptomatic dogs. In Sweden the prevalence of Giardia in healthy dogs is not known though. In this study, faecal samples were collected at two separate occasions from 21 litters and 21 bitches showing no symptoms from the gastrointestinal tract. The samples from each litter were pooled but the samples from the bitches were analyzed individually.

Infection with the bovine lungworm - Dictyocaulus viviparus - is occasionally a problem in Swedish cattle herds. The infection could act in synergism with other infections such as Corona virus to create a poorer welfare and productivity in cattle. Studies have shown that a diagnosis can be made with both the Baermann method and with an ELISA. In Norway diagnosis of BVDV on a herd level is attained through pooled serum samples. The question was raised whether pooled serum samples could be used in a similar fashion to attain status on a herd level for lungworm.

Bakground: Puerperal fever caused by group A Streptococcus is in a global context seen as a common cause of death in childbirth.Aim: To examine the consequences of group A streptococcus infections in women postpartum and to see what a nurse can do to stop the infection with adequate nursing interventions.Method: This is a review built on 15 articles. These articles were read in full and audited with a suitable template.Result: Women postpartum has 20 times higher risk to get group A stretococcus infection as compared to non pregnant. The consequences of Group A Streptococcus infection can lead to high fever, abscesses around the uterus, sepsis, acute respiratory distress syndrome (ARDS), hysterectomy and death. Patients who share room with infected patients carries an increased risk to be infected. Curtains between patient beds can be colonized by Group A Streptococcus.

Tuberculosis is one of many infectious diseases in the world and is responsible for a large number of human deaths each year. The cause is Mycobacterium ssp included in the tuberculosis-complex, there among Mycobacterium bovis and Mycobacterium tuberculosis. Infection with M. bovis causes bovine tuberculosis in cattle which in 90 % has a pulmonary location. Humans are infected mainly by milk from infected cows but also by close contact with coughing animals or by eating raw meat.

Tuberculosis is one of many infectious diseases in the world and is responsible for a large number of human deaths each year. The cause is Mycobacterium ssp included in the tuberculosis-complex, there among Mycobacterium bovis and Mycobacterium tuberculosis. Infection with M. bovis causes bovine tuberculosis in cattle which in 90 % has a pulmonary location. Humans are infected mainly by milk from infected cows but also by close contact with coughing animals or by eating raw meat.

Each year, Heterobasidion spp. is a major cause of economic losses to forestry in the northernhemisphere, including Sweden. New results indicate that pre?commercial thinnings could be at riskfor Heterobasidion spp. infections.

To be able to give optimal care to patients in small animal hospitals, we have to have knowledge about how to prevent the spread of infection. An important part in this is hand hygiene routines. We have to clean hands whenever they are visibly dirty, and disinfect them regularly in between. Another important part is the cleaning and disinfection of the environment in the clinic, and also the cleaning, disinfection and sterilization of medical instruments. Other important parts are isolation of patients with known infections, to wear clothes with short sleeves and to wash them at least once daily, to wear gloves, having short nails and not wearing jewelry when working.

Actinomycosis is a rare, deep-seated infection of the jaw of cattle that include the progressive enlargement of bone structures. It is caused by Actinomyces bovis, a gram-positive rod found as a normal commensal of the alimentary canal in otherwise healthy animals. This paper shortly describes Actinomyces bovis and its ability to cause "lumpy jaw" or actinomycosis in cattle. It will describe its pathogenesis, diagnosis, treatment and some clinical presentations. The incidence of symptomatic actinomycosis infection is quite low in Sweden today.

Borna disease virus (BDV) is a virus with capability to cause neurological disease in several species of mammals and of ostriches. Also humans are suspected to be susceptible to the virus, as the viral RNA and antibodies against BDV have been found in some humans with psychiatric diseases, like schizophrenia and depression. In Sweden there is a disease called staggering disease in cats, associated to BDV-infection. This disease often has a fatal progress. Definite diagnosis is reached by histo-pathological and immunohistochemical examination of the central nervous system.