Background: Mastitis in dairy cattle is a common ailment worldwide. A cause of mastitis can be bacteria such as Escherichia coli. Mastitis is not a deadly ailment and sometimes the dairy cows show no symptoms but if certain virulence genes are present in the bacteria that cause the mastitis, the bacteria can be transmitted to humans and cause severe diseases. The potential presence of enterohemorrhagic Escherichia coli (EHEC) in particular would be a major concern for human health.Aim: The aim for this study was to analyze the presence of virulence genes known to be present in E.coli strains isolated from dairy cows with mastitis in Sweden.Method: A Qiagen BIO ROBOT EZ1 was used to purify DNA from 90 bacterial cultures. A panel of virulence genes were amplified and biotinylated from the purified DNA by PCR and an E.coli based DNA microarray was used to detect presumed virulence genes in E.coli.Result: There were no samples that had all the genes traditionally used to classify E.coli as EHEC or potential EHEC.

Today there are various methods to use when euthanizing horses. Earlier the use of free or captive bullet was the most common method, but in recent decades this has changed and chemical euthanasia is now considered the most common method. The chemical euthanasia has in Sweden been dominated by the use of barbiturates. Somulose® entered the market in the beginning of the 90?s as an alternative to previous chemical euthanasia.

Endocrine disruptive chemicals (EDCs) are found in many everyday products and can cause adverse effects on human health. Small children are particularly sensitive to EDCs and they are also exposed to them in a greater extent than adults. Despite this, EDCs are only banned in a few toys and childcare products. There are many sources of exposure and if no steps are taken to reduce the incidence of EDCs we may face an increasing number of people diagnosed with obesity, diabetes type 2 or various types of cancer.The Swedish Government has set a number of environmental objectives, one of which is calleda non-toxic environment. The Swedish Chemicals Agency was instructed to produce and conduct a national action plan to achieve this goal.

Itraconazole is a third generation azol, a fungicide which acts by inhibiting ergerosterol synthesis in the fungal cell membrane, and thereby disturbing fungal regeneration. It has proven to be less toxic, to have a broader spectrum of activity and to be more potent than its predecessor ketoconazole. Itraconazole is only available in therapheutics approved for human use. Pharmacokinetic studies has been performed in humans, dogs, cats and laboratory animals. Although itraconazole has been used tentatively with good effect against fungal infections in horses, no pharmacokinetic studies have yet been done.

The presence of cystic ovarian follicles represents an important ovarian dysfunction and is a major cause of reproductive failure in dairy cattle. A cystic ovarian follicle is a persistent follicular-like structure on one or both of the ovaries. It is at least 2,5cm in diameter and it has to persist for at least ten days while a corpus luteum is missing. There are two kinds of cystic ovarian follicles, those who have a progesterone production and those who have not. A clear definition for this condition is lacking, probably because the heterogeneity of the clinical signs and the complexity of the disorder.

Public concern about the release of toxic compounds from wood-based products drives legislation towards enforcing industries to find and use more sustainable solutions for wood treatment. Plant oils are good alternatives to treat wood, minimizing or even neutralizing the impact on the environment. Wood modification with epoxidised linseed oil (ELO) and acetic acid as catalyst has proven a suitable method to impregnate and protect wood. However, the mixture presents a serious inconvenience because of the quick polymerization of the ingredients that limits its practical application. Therefore, this study was designed to evaluate an alternative method using a two-step process which bypassed the problem above.

Cuttings of Lonicera xylosteum L. were treated with willow extract to study the response in rooting capacity. The extracts were prepared by mixing chopped pieces of Salix x smithiana Willd. with water in a warring blender. The investigation comprised 2 types of salix extracts based on differences and dynamics between twigs in winter dormancy and twigs with new shoots. To explore the rooting response, three tests were made.

The environmental work in Sweden has developed a lot over the past fifty years. Today, most companies have some kind of environmental work. Environmental activities are regulated by the Environmental Code, which applies in parallel with other laws. This report is an environmental review for Bräcke Diakoni. The report aims to find out how much environmental impact Bräcke Diakoni has.

This thesis deals with the design of a combinated rescue-, personal-, and firefighting elevator for a nuclear waste storage facility to be constructed by the client SKB, Svensk Kärnbränslehantering AB. SKB:s mission is to dispose of all nuclear waste from all swedish nuclear power plants. The storage facility will be built in the rock 450 m under the surface and will consist of several kilometers of tunnel where capsules containing the nuclear waste will be placed. It will also hold a central area which contains spaces necessary for the operation and construction of the facility, such as elevator hall, storage hall etc. The facility will have two ways of access: a vehicle ramp and an elevator shaft.

Immune stimulating complex (iscom) matrix is a formulation that is used as anadjuvant within research. The capability of the iscom matrix to elicit an immuneresponse when not present together with an antigen has not been extensivelyinvestigated. One way to evaluate the status of the immune system is to measure theamount of cytokines that usually are produced by various cell types during an immuneresponse. The aim of the present study was to evaluate the early immune responses inporcine peripheral blood mononuclear cells (PBMC) after stimulation in vitro with theiscom matrix, AbISCO®-100. The immune response was evaluated by measuring themRNA expression for various cytokines, a chemokine receptor (CXCR4) andregulator of G-protein signaling (RGS16) in porcine PBMC after stimulation withAbISCO®-100 or other inducers like a lipopolysaccharide (LPS) and a CpGoligodeoxynucleotide (ODN 2216) used as controls.

Bipolar disorder is a severe, affective illness which causes a person to alternate between episodes of pathologically elevated mood (mania) and depression ? in between these episodes the mood is normal. The length and intervals between the episodes are highly variable among the ill, as are the severity which is divided into two major subgroups: bipolar I and II. Bipolar I disorder is the most severe because of the patient?s inability to realize that he or she is currently in a manic phase, in the case of bipolar II disorder, the patient is aware that he or she has an abnormally raised mood, a state which is called hypomanic state instead of manic.

Russian comfrey, Symphytum x uplandicum, was tested as a plant nutrient source in a trial with tomatoes. Comfrey liquid was produced by Stiftelsen Holma i Höör. Tomato plants of the cultivar Aromata planted in a medium of soil:peat (1:1) enriched with organic nutrients and lime, were fed with comfrey liquid plus Biofer, in three different concentrations. Two different concentrations of BioRika were used as comparison, along with an unfertilized control group. In the comfrey treatment I a total amont of 1g N, 0,23 g P, 0,84 g K, was given to the tomatoes.

Penicillin G (benzylpenicillin) is the most commonly used antimicrobial substance used in equine medicine today. Two forms of penicillin G are available for equine use; one form is the poorly soluble penicillin G procaine and the other is the very soluble sodium salt. Although penicillin has a wide safety margin and is considered to possess a good tolerance, reactions due to side effects are sometimes encountered, which commonly is named ?penicillin-shock?. The etiology behind penicillin shock in horses is not completely understood, but the most common reason is believed to be procaine toxicity.

To date, the genus Helicobacter consist of 26 different species and even more species are yet to be characterized. Helicobacter has been detected in connection with diseases in the liver and pancreas in humans and animals. Helicobacter spp. are gram negative, curved, S-shaped or spiral shaped bacteria. They are microaerophilic and have flagella that make them motile. Helicobacter, present in the gastric mucosa neutralize the gastric acid through production of urease that hydrolyses urea to ammonia and bicarbonate.

Staphylococcus aureus är både den vanligaste mastitorsakande bakterien hos svenska mjölkkor och den bakterie som orsakar flest matförgiftningsutbrott hos människa världen över. Utveckling av tekniker som möjliggör subtypning och genkaraktärisering av S. aureus-isolat har möjliggjort stora framsteg inom S. aureus-epidemiologin på senare år. I Sverige är det mycket vanligt att kalvar utfodras med mjölk som kan innehålla S.