Rotningsstimulerande effekt och kemisk analys av vattenbaserad extraktion av vedartade och örtartade skott av Salix smithiana Willd.
Rotning,extraktionHplcSalixEllagsyraCatechinD-salicinSaligeninKromatogramRotningsprocessSalixextraktIBA
Cuttings of Lonicera xylosteum L. were treated with willow extract to study the response in rooting capacity. The extracts were prepared by mixing chopped pieces of Salix x smithiana Willd. with water in a warring blender. The investigation comprised 2 types of salix extracts based on differences and dynamics between twigs in winter dormancy and twigs with new shoots.
To explore the rooting response, three tests were made. First, cuttings were treated with extract from salix in comparison with a control treated with water. Secondly, a rooting test was made with cuttings treated with extract from salix, IBA and the combination IBA + extract from salix. Finally a rooting test were made to assure that the extracts analyzed in the laboratory were complete and not missing any fraction important to rooting capacity that might have stayed in a column.
Culturing tests showed that salix extract from winter twigs gave more roots than a control but the roots were not longer. The combination with IBA and salix extract (50/50, IBA 2500 ppm) gave white, thin, well divided roots while plain IBA solution (5000 ppm) gave short yellowish roots.
The culturing test also showed that there is a difference between extract from winter twigs and extract from new shoots.
Chemical analyses on the salix extract were made by HPLC ? DAD combined with a fluorescence detector.
Ester hydrolyses was performed on all samples. Detection was then possible and certain chemical substances were distinct.
The following substances were found in salix extract: D-Salicin, Catechin, Ellagic acid and Saligenin. D-Salicin was found only in extract from winter twigs. One of the main substances in the salix extract revealed by the HPLC ? DAD analysis could not be identified.
Further experimentation would be interesting. Primarily, the combination IBA and salix extract should be tested on cuttings from plants normally difficult to root such as birch, beech walnut etc.
Substances in salix extract which could be interesting are salicin, saligenin and their metabolites.
Increased knowledge in this area could be very important and useful in future horticulture.