This study was conducted at the City Abattoir in Kampala, Uganda to evaluate the cleaning process, with focus on bacterial pathogens, in a pilot scale integrated bioprocess. This consisted of four steps: anaerobic sequencing batch reactors; aerobic/anoxic sequencing batch reactors; a high performance temperature controlled methanogenesis digester and a constructed wetland. The objective was to determine if this type of cleaning process could be used to clean wastewater from abattoirs in a satisfactory way. The indicator organisms for faecal contamination, Escherichia coli and Enterococcus spp. were used to investigate in what numbers bacteria could be found in the untreated wastewater and how much that was reduced in the different steps of the cleaning process. The water was analysed for Salmonella to see if human pathogens could be found and could survive the different steps of the treatment process.

The aim of this study was to detect antibodies in swine serum against proteins in soy beans, wheat, oat and barley. Two methods were used: agar gel immunodiffusion test (AGID), and SDS-PAGE followed by immunoblotting. The sera examined originated (I) from piglets younger than one week (negative control), (II) pigs from a feed trial with soy bean in a herd with PMWS, and (III) pigs inoculated with Brachyspira hyodysenteriae and Escherichia coli after being fed with soy. The AGID-test was negative concerning all examined sera which were analysed against proteins in soy bean, wheat, oat and barley. In the experiment based on immunoblot antibodies were demonstrated within all three groups of examined pigs.

A group of diseases that affect the dog?s sight is progressive retinal atrophy (PRA) a disease that means that the photoreceptors in the retina of the eye either not develop normally or undergo degeneration and thereby causes vision loss and blindness. The disease affects many different breeds and is inherited. What have been shown is that it is different genes that had become mutated in the different breeds, this conclude that despite similar clinical symptoms, the underlying reason could be different. Also the genes that are mutated in dogs and give rise to diseases also exists in humans.

Denna rapport beskriver skapandeprocessen av webbsidan aiducate.se, vars syfte är att stötta, motivera och vägleda gymnasieelever i sin utbildning. Projektet genomfördes självständigt utan någon uppdragsgivare och pågick under en tidsperiod om tio veckor. Till grund låg en förundersökning som visade på bristande motivation hos gymnasieelever. I en kompletterande enkätundersökning fastställdes det att eleverna önskade en modernisering av utbildningen med utrymme för nutida undervisningsmetoder. Som ett komplement till gymnasieutbildningen framställdes därför innovationen Aiducate som i framtiden ska omfatta olika verktyg för studierna.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

Verotoxinproducerande E. coli, VTEC är en zoonotisk bakterie som kan orsaka allvarlig sjukdom hos människa. Forskning kring VTEC har framför allt varit inriktad på nötkreatur vilka tidigt utpekades som en viktig smittkälla för människa. Förhållandevis få studier har gjorts på får och fårprodukter och kunskapsluckorna är stora både vad gäller förekomst och utbredning av VTEC hos detta djurslag. Syftet med denna studie var att utreda om får kan utgöra en smittkälla för människa vad gäller verotoxinbildande E.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

The bacterium Lactobacillus reuteri, which natural habitat is in the intestine of mammals and birds, uses glycerol as a substrate for production of the antimicrobial compound reuterin. Glycerol has been shown to decrease the number of Escherichia coli in human feces and it is believed to be due to in situ production of reuterin. E. coli belongs to the large family of Enterobacteriaceae, naturally occurring in the intestine, and pathogenic strains of E. coli have been shown to be one of several bacteria causing diarrhoea in calves.

Syftet med denna magisteruppsats a?r att ga? vidare med resultat fra?n en kandidatuppsats (Westling, 2013) ga?llande opasto?riserade franska mo?gel- och kittostar genom att underso?ka vilka Enterobacteriaceae-arter som ett urval av de analyserade ostarna inneho?ll. API 20E anva?nds som identifieringssystem. Tre Enterobacteriaceae-arter gav acceptabel till utma?rkt identifiering av 40 analyserade isolat fra?n de fyra ostarna, na?mligen Hafnia alvei, Escherichia coli och Klebsiella pneumoniae.

Syftet med denna magisteruppsats a?r att ga? vidare med resultat fra?n en kandidatuppsats (Westling, 2013) ga?llande opasto?riserade franska mo?gel- och kittostar genom att underso?ka vilka Enterobacteriaceae-arter som ett urval av de analyserade ostarna inneho?ll. API 20E anva?nds som identifieringssystem. Tre Enterobacteriaceae-arter gav acceptabel till utma?rkt identifiering av 40 analyserade isolat fra?n de fyra ostarna, na?mligen Hafnia alvei, Escherichia coli och Klebsiella pneumoniae.

The canine population has during it?s time with humans gone through at least 2 major genetic bottlenecks, the first when it was separated from the wolf, and the second when it was divided into different breeds. Among other things, this has lead to the accumulation of unwanted genes, such as genes causing disease in dogs. One such inherited disease is syringomyelia, which has proven to be more common than expected, especially in certain smaller breeds such as the Cavalier King Charles Spaniel. Syringomyelia in dogs is often a secondary condition caused by the malformation called occipital hypoplasia.

A research of 14 toxic affected study sites in Sweden showed a significant increased proportion of deformed diatoms. The six metal polluted study sites in the region of Dalarna showed a 4, 2% average of deformed Fragilaria (deformed diatoms reference sites, 0, 1%). The results also indicate a significant increased proportions of deformed diatoms in the eight pesticide polluted sites in the south and middle of Sweden, compared to the reference sites, howsoever the toxic content was low. The results indicate that there are no lager differences in neither teratological forms nor variations that segregate metal polluted and pesticide polluted shells. One of the reasons may be that both heavy metals and pesticides can affect the development of the diatom shell..

Marknaden för terapeutiska proteiner kan grovt delas in i 2 områden: de proteiner som inte modifieras efter translationen och de som kräver posttranslationell modifiering (bl a glykosylering) för att vara biologiskt aktiva. Eprex®: humant rekombinant erytropoietin är ett glykoprotein som används vid anemi, det stimulerar mitos och differentiering av stamceller i benmärgen till mogna erytrocyter. Erytropoietin är bara ett av många läkemedel för humant bruk som tillverkas i mammalieceller. Mammalieceller är de som är lämpligast att använda som uttryckssystem för terapeutiska glykoproteiner tänkta för humant bruk. Detta eftersom de innehåller de organeller och enzym som krävs för att erhålla korrekt proteinkonformation och utföra avancerade posttranslationella modifikationer.

Virulent strains of Staphylococcus hyicus produce exfoliative toxins and cause a potentially fatal skin disease in piglets known as exudative epidermitis. Vaccines and antimicrobials are used to control the disease. Antimicrobial resistance and the prevalence of several different toxin genes and toxigenic strains make it problematic. The aim of this literature study was to identify potential differences between countries and between healthy and diseased pigs regarding antimicrobial resistance and toxin-producing strains. Great differences could influence vaccine production, treatment strategies and surveillance programs.

Det är populärt att gå ut och äta pizza, och en vanlig ingrediens på pizza är köttfärs. Köttfärs tillhör gruppen lättfördärvliga livsmedel och märks därför med sista förbrukningsdag (SLV, Vägledning, 2011). I dagligvaruhandeln får köttfärsen inte säljas efter sista förbrukningsdag, vilket i många fall bara är en dag framåt om det är butiksförpackad köttfärs. Köttfärs som är förpackad i modifierad atmosfär har längre hållbarhet, men när förpackningen bryts försvinner den skyddande atmosfären. Inspektörerna på miljökontoret i Uppsala har märkt att många pizzerior använder ett öppnat paket köttfärs i flera dagar.