Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .

In this paper my aim is to understand the trance movement from within as a collective phenomenon. I went to Turkey with my boyfriend David on a trance gathering, a festival called A Total Solar Eclipse Festival. There I did participant observations and interviews.Can Collins theory about interaction ritual be useful to raise the understanding? And is it fruitful to apply the term collective identity?The collective identity is build up by the festival as interaction ritual. It generates solidarity, emotional energy and a sense of belonging.In this paper I discuss different categories and symbols that the collective identity contains of.

The aim with my study is to highlight, illuminate and examine the social and maternal health care motivational work of pregnant women with a risk or abuse of alcohol and / or drugs, and what actions can be considered for these women. In addition, the aim is to explore how social services and maternity care might interact in this type of case. The aim is to convey the subjective experience of each of the interviewees. For the purposes of this study, I have chosen to use the qualitative research method. I have interviewed social workers in individual and family care, and midwives.

Each year, 800 people wait for an organ transplant in Sweden. The development of drugs for organ rejection has evolved over the past 60 years, which has increased the survival of patients who have undergone organ transplantation. The most common organ transplanted is the kidney, liver, lung and heart. There are more studies done on quality of life after an organ transplant, but very few studies on quality of life before organ transplantation.The aim of the study was to describe the experiences of quality of life among patients waiting for an organ transplant.A literature overview of eight articles was analyzed in both similarities and differences. The articles were published between 2002 and 2012.The result is presented in four themes, hope of life, living in uncertainty, the body fails and need for support.

This study is an examination of how conditions for formal and informal music education look like in two townships in South Africa. The purpose of this examination based on different actors' experiences is to find out whether the children in South Africa receive some form of music education and if so, how they think it can help the children in the surveyed areas. The information was gathered both through qualitative, semi-structured interviews with a music teacher, music therapist, creative music facilitator, students, and people who have worked for Sida and partly through unstructured observations. The results show that children in the surveyed townships do not receive any formal music education through the school but they sometimes can get music education through a NGO project (Non Government Organization) which usually runs by an enthusiast and is funded by various aid agencies. My examination also showed that for the children in the surveyed areas music education can be an alternative from being on the streets, giving a positive group experience, the ability to express themselves, fend off young people from drugs and criminality and bring together various grouping.

Syftet med denna studie är att undersöka drogturismen från ett fenomenologiskt perspektiv, alltså försöka förstå fenomenet som den upplevs av aktörerna. Baserad på en fältstudie där jag genomför intervjuer så är målet att kunna förklara hur och varför drogturism uppstår. Intervjuer har genomförts med individer som har rest till Barcelona under olika tidsperioder och tagit del av drogscenen. Tidigare studier har visat att semesterresor är vanligt förekommande för att initiera ett drogbruk. En hypotes avseende orsaken till droganvändandet är att resan till semesterorten skulle vara en medveten handling som genomförs med syfte att använda droger, s k drogturism.

Barley is one of the most important cereals cultivated in the Nordic countries. Climate change brings warmer and moisture climate which favors fungal diseases. In the cropland barley can be seriously infested with hard fungus attack. Since it is important that the yield bears a high quality it is of great importance to find varieties more resistant to attacks.Pathogenesis-related (PR) proteins are stress proteins induced in the plant in response to infection and abiotic stress (van Loon, 1997). PRs are shown to have antimicrobial activity differing between bacteria, fungi and oomycetes (Tandrup Poulsen, 2001).

Barley is one of the most important cereals cultivated in the Nordic countries. Climate change brings warmer and moisture climate which favors fungal diseases. In the cropland barley can be seriously infested with hard fungus attack. Since it is important that the yield bears a high quality it is of great importance to find varieties more resistant to attacks.Pathogenesis-related (PR) proteins are stress proteins induced in the plant in response to infection and abiotic stress (van Loon, 1997). PRs are shown to have antimicrobial activity differing between bacteria, fungi and oomycetes (Tandrup Poulsen, 2001).

This masters degree is a study of factors of success in a drug preventions work of change and model or method which could facilitate this work. The target group, which is in focus in this survey, is alcohol- and drug coordinators, also called prevention coordinators who?s main task is to develop and coordinate the preventive work in the alcohol and drugs area. My method of investigation is a combination of literature studies and a quantitative poll study with the target group, alcohol and drug presenters?, in Sweden.

Statistics shows that drunk driving and relapse increases in the Swedish society and it?s a problem that can be considered worth examining because of all the negative consequences that it conveys. To reduce the number of relapse it?s urgent that individuals that have been drunk and driving begins treatment and precedes it. The purpose of this study was to, from a client and therapeutic perspective, examine why drunk drivers begin treatment for their alcohol abuse and furthermore to investigate what?s important for the individual so that he completes the treatment.

BackgroundResearch shows that the majority of patients feel anxiety before and during anesthesia. It has been shown in several studies that preoperative anxiety may cause adverse effects in the postoperative course. Nurse anesthetists have a key role in creating a safe environment and the opportunity to use different interventions to reduce the degree of anxiety.AimThe aim of the study was to examine which interventions may reduce anxiety in adult patients before and during both general, local and regional anesthesia.DesignSystematic literature review with a quantitative approach.ResultsSeveral studies indicate that gabapentin and pregabalin can reduce anxiety before and during anesthesia. The anxiety reducing effect appeared to be dose dependent. The naturopathic drugs Citrus aurantium blossom and Passiflora incarnata Linneaus were found to reduce anxiety before anesthesia.

This literature study aims to examine the existence of cancer stem cells in breast cancer. The cancer stem cell theory states that there is a hierarchical organization within a tumour, in which a small subpopulation of the cells can initiate new tumours and maintain tumour growth whilst the bulk of the tumour cannot. These tumour initiating cells have shown to possess many characteristics similar to those of adult stem cells, which is why they are often referred to as cancer stem cells. Both cell types have the capacity of asymmetric division and have shown to possess mechanisms of resistance to both apoptosis and cancer drugs. The cancer stem cell theory elucidates many biological aspects such as the heterogeneity of tumours and the relapse of many cancers after what appeared to be successful treatments.

Drug related messages in antidrug campaigns and music related media -A qualitative study about young people?s perceptions Youth are exposed to both promotional and critical drug and alcohol messages in their daily media consumption. The purpose of this study is to examine how youth perceive anti-drug campaigns and drug-promoting messages in music-related media. The empirical material consists of qualitative data, including a focus group and two individual interviews, consisting of youth between the ages of 18 and19. One main finding is that young people perceive anti-drug messages differently, yet they are virtually unanimous about what makes such messages effective.

Propofol is an intravenously administered, hypnotic and short- acting pharmaceutical. One common sideeffect (>1:10) and therefore a disadvantage with propofol is the local pain that arise when the initial injection is given. Why the pain arise is not clearly understood. A majority of different pharmacological treatments, different doses and combinations, alternative administrations methods and physical interventions have been tried to reduce the pain when injection of propofol is given. One important task for the nurse is to relieve pain for patients.

Staphylococcus pseudintermedius (S. pseudintermedius) är en opportunist som ingår i hundars normalflora och den bakterie som oftast orsakar pyodermi hos hund. En alltför frikostig användning av antibiotika inom veterinärmedicinen har selekterat fram nya resistenta bakterier som meticillinresistenta S. pseudintermedius (MRSP). Det är en bakterie som i vissa fall visat sig vara resistent mot alla större antibiotikagrupper som används inom veterinärmedicinen.