The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

Otitis externa is a disease regularly encountered in veterinary practice. It is estimated to be the most common disease in the external ear canal of dogs and treatment is often delayed due to unfortunate pet owner ignorance. Owners tend to assume that scratching of the ears and head shaking are natural behaviours in their dog and may be neglecting the problem unknowing of how severe it actually is. A literature study has been carried out to examine the causes of otitis externa in order to present a collection of measures that can be used in the treatment and prevention of the disease. In turn these measures can be advised to the dog owner and used in the home environment to make the best qualifications for healthy ears in the dog. The causes of otitis externa have been divided into predisposing, primary, secondary and perperuating causes.

Författare: Malin Pettersson & Emma SundåkerTitel: ?Typiskt manlig? Då tänker jag på fotbollsspelare som sjunger nationalsången?: En studie om fyra manliga sångares berättelser om sång, självkänsla och maskulinitetTitle in English: ?Typically masculine? Then my thoughts go to football players singing the national anthem?: A study about four male singers and their stories about singing, selfesteem and masculinityBåde för män och kvinnor är det problematiskt att inkräkta på ett fält som domineras av det andra könet. Populärmusikforskning med genusperspektiv har ofta fokuserat på kvinnor som befinner sig i maskulina praktiker, vilket därmed gör oss nyfikna på det motsatta. Att som ung kille våga erkänna att man tycker om att sjunga kräver mod samt självkänsla och självförtroende eftersom han trotsar de traditionella könsrollerna. Vi vill undersöka hur de män som har sång som primärt instrument förhåller sig till att deras instrument kan associeras till femininitet.Syftet med uppsatsen är att undersöka hur manliga sångare beskriver sin röst och röstutveckling, samt hur de hanterar att de verkar inom en feminin praktik.Uppsatsen bygger på fyra manliga sångares skrivna levnadsberättelser och är skriven ur ett genusperspektiv med fokus på maskulinitet inom populärmusiken.

Denna uppsats syftar till att utveckla en förståelse för hur stråkinstrumentundervisning kan bedrivas då syftet är att lära ut en ergonomisk riktig spelteknik som förebygger hälsoproblem. Fokus i studien har varit på nybörjarundervisning samt HUR elever spelar. Studien har genomförts med hjälp av kvalitativ metod. Sex ergonomiskt kunniga och intresserade musiker och pedagoger har intervjuats. Deras specialområden är musikerergonom, sjukgymnast, kontrabasist, forskare, alexandertekniklärare och cellopedagog.

The aim of this study was to explore how young students can be taught during their first year of learning English towards the A1 level according to The European Framework of Reference for Languages, and how the students understand their own learning. The study was carried out in two different classes but with the same teacher, who is qualified to teach English to young learners. The result is based on the analysis of a total of twelve observations of English lessons in two classes in school year one and eight group interviews with the students. Recurrent ingredients through all lessons were activities such as listening to teacher talk, watching films or film clips, singing songs and doing rhymes, using drama activities, talking about the meaning of learning English, practicing guessing competence and learning new vocabulary. The teacher consistently talked about and taught different strategies for language learning.

Detta är en fallstudie som handlar om arbetet med en sångelev som påstod sig inte kunna sjunga. Eleven hade byggt upp stora mentala hinder kring sin sångförmåga, och studien tar upp dessa hinder samt de processer som utlöstes vid försöken att komma runt dem.Syftet med studien är att undersöka tankegångarna kring undervisningen av eleven samt de övningar som användes. Studien belyser också de processer eleven och läraren gick igenom samt diskuterar deras betydelse och innebörd.Studien bygger på lärarens anteckningar från 30 lektionstillfällen, utspridda på ca två år, samt lärarens och elevens reflektioner efteråt.Resultatet visar några av de processer som bildades under arbetets gång, samt att eleven kunde klara av mer än hon trott var möjligt genom att utveckla rätt inställning och ha tillgång till bra verktyg..

I studien har en relativt ovanlig intervjumetod, Repertory Grid Technology använts för att ge några blivande fysiklärares syn på god fysikundervisning. Metoden inspirerar till användning i många andra sammanhang. Resultaten visar att god fysikundervisning bland annat uppfattas som krävande för läraren..

The purpose of the present study was to study if the systemic blood pressure in cats significant increases due to stress associated with a visit to a veterinarian clinic. We also wanted to investigate the clinical utility of two different methods for indirect blood pressure measurements in cats by assessing how easy they were to use and how reliable measurements they provided. The methods that were used were oscillometric (Memo Diagnostic) and ultrasound-doppler technique (Minidop). The blood pressure was measured in both clinical environment and home environment. Fifty cats were used in the study, from which measurements were impossible to obtain in one cat.

The overarching purpose of the study was to examine the effects of infant massage and to put these effects incontext with mother ? child attachment. The intention was also to study whether these effects have any relevancefor social work. This was accomplished by studying earlier research, theory and laws but also through interviewswith mothers who have followed through a course in infant massage. The main questions of the study was: Afterthe completion of the course and after using the massage technique at home, does the mother experience that thebond with their child is influenced by the technique and are the mothers more apprehensive of their child?ssignals? Has the mothers observed any signals from their infants during the massage and if so what has beentheir reaction? Does the mothers experience that the massage has provided any physiological effects? Has thecompletion of the course provided the mothers with knowledge that?s had an effect in their ways of parenting? Inwhat way does the earlier research, reports and laws deal with the benefits and risks of a secure or insecureattachment, and can the quality of the attachment have any meaning in the social work? A qualitative approachwas chosen with respondent-interviews.

The purpose of this essay is to establish whether the so called ?soup trilogy?, med en burk soppa (2001), konservöppnare bok (2003) and på burklös mark (2005), written by Swedish-speaking Finn Stefan Hammarén is to be considered as surrealism or not. The methods used to determinate this is close reading, a technique used for finding a text?s distinguishable traits, and comparative analysis. First of all, the criteria of surrealist literature found in André Breton?s (1896-1966) Surrealist Manifesto, and other sources, as well as the criteria for the surrealist literary technique cut-up are presented and evaluated accordingly.

This work is about getting to know tufting as a technique and to design tufted products for home furnishing.It all began in Finland, where during springtime 2004, I got in contact with the technique oftufting for the first time. A very pleasant meeting that resulted in two carpets and a longing to continueand learn more. It was then that an idea for my final project started to take form. My wish was to designtufted ?floor jewelleries? and cushions.I made a base for my project during a design project in autumn 2004.

Rising complexity in the development of real-time systems has made it crucial to have reusable components and a more flexible way of configuring these components into a coherent system. Aspect-oriented system development (AOSD) is a technique that allows one to put a system?s crosscutting concerns into"modules"that are called aspects. Applying AOSD in real-time and embedded system development one can expect reductions in the complexity of the system design and development. A problem with AOSD in its current form is that it does not support predictability in the time domain.

Objective: In recent years there has been an increasing interest within the clinical (medical) science in measuring people?s health. When estimating quality of life, present practise is to use the EQ-5D questionnaire and an index which weighs the different questions. The question is what happens if the individuals estimate there own health, would it differ from the public preferences? The aim is to make a new prediction model based on the opinion of patients and compare it to the present model based on public preferences.Method: A sample of 362 patients with unstable coronary artery disease from the Frisc II trial, valued their quality of life in the acute phase and after 3, 6 and 12 months.

From a view of health strategies and techniques this thesis wants to throw a light on how the individual in its"health thinking"is governed. A thinking that, according to the author, can lead to a new way of governing as in municipal activity, an activity whose focus is on health and ill-health. The study is done whit a theoretical approach of power, with qualitative interviews as method and analysed from a perspective of Governmentality. The object for the study is Socsam and Rehabrådgivarna in Finspång. Socsam is a local project, and their main task is to create cooperation between health- and medical services, social services and social insurance.

There is an old saying, that after Epaminondas nothing was ever the same again. In this thesis I will focus on three questions: "Epaminondas the man." Who was he? "Epaminondas the general." How did he change the technique of warfare that made it possible to beat the Spartans? "Epaminondas, founder of cities and wall-builder." How did he build the walls around the cities he initiated, that made them resist enemy attacks for many years to come?While seeking the answers to yhese questions I will make a contextual analysis to get the historical background to all the phenomina involved..