The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

?Death in the pot.? The Fruit and Vegetable Preserving industry in East Scania during the 20th centuryA Company History.At the end of the twentieth century, only one of the six companies previously active in the Fruit and Vegetable Preserving Industry in East Scania survived as an independent company: Kiviks Musterier AB. Actual production remains at two sites only. The aim of this study is to determine the reasons for this development. The companies included in the investigation are AB Bjäre industrier, Gustaf Bong AB, Kiviks Musterier AB, AB Ruuthsbo konservindustrier, Th.

Background: Phonemically balanced word lists are used when obtaining speech recognition scores in noise. These lists are designed for adults, but are still used for children. To properly obtain speech recognition scores in noise for children, normative data is needed to show what differences there are to be expected between children of different ages. Purpose: The main purpose is to obtain normative data for children in the ages of 7, 10 and 13 years for speech recognition scores in noise using words and to compare these with each other and with normative data of adults. A further purpose is to examine if there is any practice- or exhaustion effect to be seen when obtaining speech recognition scores in noise for children in the ages of 7, 10 and 13 years.Material: The participants were ten 7-year-olds, ten 10-year-olds and ten 13-year-olds.

The purpose of this Master?s thesis is to perform a user oriented evaluation of the International Library?s website. The questions posed in this study are; for what purpose do the users in this study turn to the International Library?s website; what does the users information searching process consist of when visiting the site; how does navigation, layout and content effect the users satisfaction with the website; do the users in this study consider that the International Library?s website can be improved and if so, how? The study was performed with a user oriented approach where six qualitative interviews with librarians who use the website were conducted. These interviews were analysed using Brenda Dervin?s Sense making-theory and Leclercq?s theory about user satisfaction.

The aim of this study is to describe and analyse an elderly care team?s prerequisite to anchor a salutogenic perspective and approach in an elderly care team in Kalmar municipality. The study identifies factors that could impede or promote the anchoring and examine how the organisation aims to work in a single direction even if the multidisciplinary team processes a diversity of knowledge, education and abilities. The study is based on qualitative semi-structured interviews from representatives of all professions in the multidisciplinary team; social workers, paramedical professions, nurses and care-givers. The theoretical frame consisted of a translating perspective, leadership and ideas of interaction and power.

The purpose of this study was to examine how five mothers experience and cope with theloss of their adult children and also how this loss affect the mothers. We chose a qualitativemethod using semistructured interviews. We used Lazarus and Folkman?s coping theoryand Folkman?s revised coping theory in our analysis. The results showed that the mothers?experiences were very individual.

Domestic violence is a serious public health issue that concerns everyone. Public health service is one of many authorities where battered women seek help. The purpose of this literature review was to describe which factors affect the encounter between battered women and nurses in public health departments. The method consisted of analysing 11 scientific articles. The result is presented in themes that show the different attitudes in the encounter between nurses and battered women.

This is a qualitative study with the aim of getting better knowledge and understanding of how people with mental illness experience having access to a public communication channel. The study builds upon qualitative interviews with four participants of a radio project called Radio Totalnormal (RTN). RTN is a community radio station where people with mental illness do radio. The study focuses on how the respondents describe the participation in the project and how it has affected their self-image. The analysis uses the concept of empowerment and four social psychological concepts: social responsivity, asocial non-responsivity, abstract sociability and concrete sociability.

Cancer was the second most common cause of death in Sweden in 2011. As the number of beds in hospitals decreases, numerous severely ill patients receive care at home. This results in family members performing important parts of the caring process. The aim of this study was to describe the caregivers' support needs from healthcare professionals when caring for an adult relative with cancer. A literature review was performed, in which results from empirical studies were reviewed and summarized.

Background: Every year 56 million people die around the world and it is estimated that 60% of these people are in need of palliative care. It has been shown that patients in the palliative care experienced their situation as uncertain. When life no longer was certain, the patients tried to live their life day by day and make the best of it. Problem: When patients were diagnosed with an incurable disease the future became unsure, because their life would come to an end. Therefore patients with a palliative diagnosis could be in need of experiencing feelings of security.

This paper investigates the grammatical construction intransitive motionconstruction with GOAL as it is used in Swedish (Swe. intransitivrörelsekonstruktion med MÅL).I examine in which ways the construction is productive, i.e. thepossibility to use the construction with new verbs as well as withordinary motion verbs with new functions. The notion of productivity isbased on the construction?s type frequency and semantic openness(Goldberg 1995, 2006; Barðdal 2008; Bybee 2010).

ADHD (attention deficit hyperactivity disorder) is a phenomena that is much talked about in both schools and in communities. Today, there is a conflict surrounding the diagnosis ADHD. A part of the conflict takes part between the sociologist Eva Kärfve and Christopher Gillberg, professor in child- and teenage psychology. The roots of this conflict surrounds the much debated line of what is seen as normal and ?too? different, basically how many children that actually should have the diagnosis.

The purpose of this study was to increase the knowledge and understanding of what impact support-groups, such as represented by Qvinnoqulan, can have on women with drug-addiction. The questions at issue for the essay was: How does a selection of women who participate in Qvinnoqulan describe their thoughts, experiences and reflections of the group? What sense has Qvinnoqulan made for the women on a personal level and for contributing to live a life without drugs? To answer the questions a qualitative method was used and five interviews were made with women who participated in the activities of Qvinnoqulan. To analyze the data a social constructionist perspective and an empowerment perspective was used. The result of the study showed that the women were in the process of rebuilding a new life without drugs and reconstructing a new identity.

In which ways have composing processes in music appeared over the past 300 years? How may composing processes work today? What does new technology do to this processes?The key purpose of this study is to investigate various composing processes. A further purpose is also to gain an awareness of how we use modern technology by computers and their notation programmes.The research design of this thesis includes a display of literature related to composing processes. along with an analysis of the writer?s own experiences in the area.

The purpose of this essay is to illustrate how the position of power / exercise of power are perceived in today's schools from the teacher's perspective. I have examined how students' freedom and democracy education interpreted in real life, and how this affects both teacher and student learning approaches. I would also highlight the need to set standards for children's psychological development related to the consequences will be. I have chosen to perform a qualitative research through individual interviews with five participating teachers from different experiences in different type of school. I have used the hermeneutic method of analysis to analyze the material.