Bakgrund: Patient i hemodialys (HD) behandling är utsatt olika stressfaktorer, som tillexempel den strikta diet, begränsat vattenintag, bundenhet till dialysenhet eller känsla avtrötthet även på dialysfria dagar. Cirka 20 procent patienter i HD behandling utvecklardepression som i sin tur leder till låg Plasma albumin.Syftet: Att belysa samband mellanstressfaktorer, depression, låg Plasma albumin och mortalitet/morbiditet samt omvårdnadensbetydelse för patienter i hemodialysbehandling.Metod: Studie genomfördes som enlitteraturöversikt och granskning av vetenskapliga artiklar.Resultat: Resultat baserar på 15artiklar, varav 1 kvalitativ, 14 kvantitativ. Resultatet delades i fem kategorier: stressfaktoreroch deras koppling till depression, depression, låg Plasma albumin, omvårdnadsåtgärder vidlåg Plasma albumin, omvårdnadsåtgärder vid depression.Diskussion: I diskussion lyfts uppsamband mellan högre morbiditet/mortalitet och depression samt låg Plasma albumin hospatienter i HD behandling. Det tas upp även vikten av omvårdnadsinsatser vid depression ochlåg Plasma albumin hos patient i HD behandling.Slutsats: Mer forsknings behövs för attsjuksköterska kan erbjuda patienten effektiva strategier som hjälper denne att hantera destressfaktorerna och livsstilförändringar som HD innebär..

Bakgrund: Patient i hemodialys (HD) behandling är utsatt olika stressfaktorer, som tillexempel den strikta diet, begränsat vattenintag, bundenhet till dialysenhet eller känsla avtrötthet även på dialysfria dagar. Cirka 20 procent patienter i HD behandling utvecklardepression som i sin tur leder till låg Plasma albumin.Syftet: Att belysa samband mellanstressfaktorer, depression, låg Plasma albumin och mortalitet/morbiditet samt omvårdnadensbetydelse för patienter i hemodialysbehandling.Metod: Studie genomfördes som enlitteraturöversikt och granskning av vetenskapliga artiklar.Resultat: Resultat baserar på 15artiklar, varav 1 kvalitativ, 14 kvantitativ. Resultatet delades i fem kategorier: stressfaktoreroch deras koppling till depression, depression, låg Plasma albumin, omvårdnadsåtgärder vidlåg Plasma albumin, omvårdnadsåtgärder vid depression.Diskussion: I diskussion lyfts uppsamband mellan högre morbiditet/mortalitet och depression samt låg Plasma albumin hospatienter i HD behandling. Det tas upp även vikten av omvårdnadsinsatser vid depression ochlåg Plasma albumin hos patient i HD behandling.Slutsats: Mer forsknings behövs för attsjuksköterska kan erbjuda patienten effektiva strategier som hjälper denne att hantera destressfaktorerna och livsstilförändringar som HD innebär..

Background: Folate is a water - soluble vitamin necessary for normal DNA synthesis among  other mechanisms. Folate deficiency can cause megaloblastic anemia resulting from abnormal erythrocyte d evelopment. At the Department of Clinical Chemistry, Linköping, analysis of folate is performed with Siemens ADVIA Centaur® XP which uses an immunochemical method with chemiluminimetric detection. Currently, the assay is conducted on serum, is poured off and frozen after 48 h and treated with light protection because folate is considered to be light sensitive.Methods: This study tested lithium heparin Plasma for analysis of folate with Siemens ADVIA Centaur® XP with the aim to be able to perform a package of analyzes in anemia investigation from the same collection tube. Furthermore, the stability of folate was studied for up to 168 h and examined regarding light sensitivity.Results: Statistically significant higher values were obtained in the analysis of folate in lithium heparin Plasma than folate in serum (p < 0,001).

Detta arbete syftade till att validera en metod för analys av interleukin-1 receptor antagonist (IL-1 RA) i Plasma från häst. Med en fungerande analysmetod finns möjligheten att använda IL-1 RA som biomarkör för antiinflammatoriska effekter av läkemedel exempelvis dexametason. En kommersiell ELISA framtagen för analys av IL-1 RA hos häst användes vid valideringen. Analysmetoden var tidigare endast validerad för analys av IL-1 RA i spädningsmedium från tillverkaren. Vid validering av metoden framställdes standardkurvor i spädningsmedium från tillverkaren (reagent diluent=RD) samt i olika biologiska medier från häst. Beräkning av standardkurvorna utfördes med hjälp av linjär regression och innefattade intervallet 0,313 ? 20 ng/mL.

Cardiac troponin I (cTnI) is considered a specific and a sensitive biomarker of cardiac disease, and due to a high inter-species sequence homology; human cTnI assays can often be used in veterinary medicine. The main aim of this study was to perform an initial evaluation of a human cTnI test, the Meritas Troponin I test, for measurement of equine Plasma cTnI, in order to investigate whether the test could be a possible subject for a more comprehensive validation. The hypothesis was that the test could measure equine cTnI, as there is a good homology between equine cTnI and human cTnI. The evaluation included a dilution parallelism, an intra-assay precision study and measurement of Plasma cTnI healthy horses. In the dilution parallelism and the intra-assay precision study, equine Plasma with previously established high cTnI concentration, according to another cTnI assay, was used. In addition, cTnI concentration was measured in Plasma, collected from 19 horses, without signs of disease at physical and ECG examinations. The obtained curve in the dilution parallelism was linear, indicating that the Meritas Troponin I test can be used to measure different concentrations of equine cTnI in Plasma.

Clopidogrel is an antiplatelet substance that prevents blood coagulation in the arteries. It is an inactive pro drug that becomes activated after first-pass metabolism by the liver. The active metabolite of clopidogrel is 2-oxoclopidogrel, which is unstable therefore pharmacokinetic data is obtained by measuring the inactive metabolite clopidogrel acid in Plasma. Clopidogrel is taken orally in tablet form. The aim of this project was to develop a LC-MS/MS method for quantification of clopidogrel and its metabolite in Plasma. The method has been developed by optimizing the sample preparation.

A numerical simulation case involving space Plasma and the evolution of instabilities that generates very fast electrons, i.e. approximately at half of the speed of light, is used as a test bed for scientific visualisation techniques. A visualisation system was developed to provide interactive real-time animation and visualisation of the simulation results. The work focuses on two themes and the integration of them. The first theme is the storage and management of the large data sets produced.

Most companies have a bottleneck in their production, that is a constraining re-source. When the demand is higher than the capacity in the constraining resource it is impossible to produce the demanded output. This problem leads to delayed deliveries, unhappy customers and ultimately to decreased revenue. Bottlenecks therefore has a significant role in production and must be handled thereof.MW Power in Sävsjö, Sweden designs and manufactures water and steam boilers for the customer's unique needs. MW Power invested in a new Plasma cutting ma-chine but the machine has not reached the expected capacity.

Today there are no analgesic drugs approved to be used in goats. Drugs approved for use in other species are consequently being used without knowing the pharmacokinetics of these drugs in goats. The purpose of this study was to investigate the pharmacokinetics of the opioid butorphanol when given subcutaneously in goats. Subcutaneous administration might extend the half-life compared to intravenous or intramuscular administration. Eight clinically healthy, non-pregnant 6 months old female goats were used.

Vid nuvarande framställning av kontroller och kalibratorer för läkemedelsanalys med LC-MS/MS-teknik används idag serum där fibrinogenet har fällts ut med kalciumklorid. Eftersom detta tros ge upphov till ökad jonsuppression skulle Plasma där fibrinogen istället tagits bort genom frysning/tining kunna användas. Denna studie har utförts för att undersöka om och hur dessa två olika matriser skiljer sig vad gäller bland annat jonsuppression och enhancement, och studien innefattar även obehandlad Plasma. Studien har utförts i samband med analys av två olika metoder för läkemedel, som bland annat utgörs av neuroleptika och bensodiazepiner. Postkolonn-infusion utfördes för båda metoderna och ingen nämnvärd jonsuppression eller enhancement kunde påvisas för någon av de tre olika matriserna.

The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by procaine toxicity. Procaine penicillin G is a salt which is quickly dissolved in Plasma. Procaine is then metabolized by Plasmaesterases to non-toxic metabolites, p-aminobenzoic acid (PABA) and diethylaminoethanol.

Haemophilia is an inherited disease which causes increased bleeding due to defect clotting factors VIII and/or IX. There are two forms of haemophilia, A and B, which both are X-linked and due to mutations of the genes of factor VIII and factor IX respectively. This study focuses on Haemophilia A and thereby factor VIII.The drugs that are available on the Swedish market today for patients with haemophilia A include Factor VIII(FVIII), which has been purified from human Plasma and recombinant factor VIII(rFVIII). The main goal of this study is to gain a better understanding of the effects of treatment of haemophilia A with either purified Plasma factor VIII or recombinant factor VIII on reducing or preventing bleeding and on side effects.  The study was designed as a literature review and searches were carried out in PubMed at the Linnaeus University library.  The criteria for selection of articles were: patients with haemophilia, treatment with Plasma derived and/or recombinant factor VIII as well as clinical studies.

Today, some kind of laboratory results is required for around 70% of the diagnostics and follow-ups for diseases. In many of the cases the time from sampling to a result is very critical. Therefore the discussion of how to improve this situation has begun. For many analyses serum has been the routine choice for a long time but now it is disputed. After blood collection in a serum tube it is essential to wait 30-60 minutes before centrifugation and analysis of the sample, a long time for someone in an acute state.

Breeding-induced endometritis in horses seems to be down-regulated by certain components in the seminal Plasma. These components are most likely some of the proteins in the seminal Plasma in the stallion ejaculate, the so-called spermadhesins. This study aimed to recognize these proteins and determine their quantities in different fractions of the ejaculate. Fractionated ejaculates from four stallions were used to determine the presence and amount of proteins by way of size-excluding chromatography. All former known proteins, except two, were recognized in this study.

Syfte: Syftet med denna systematiska litteratur?versikt var att unders?ka det vetenskapliga underlaget kring om regelbundet intag av ?gg p?verkar niv?er av trimetylamin-N-oxid (TMAO) i Plasma hos vuxna. Metod: En litteraturs?kning genomf?rdes i databaserna PubMed, Scopus och Cochrane Library 25 januari 2023. Inklusionskriterierna var vuxna ?18 ?r, intervention best?ende av ?gg eller ?ggula, kontroll best?ende av ?ggsubstitut/?ggvita/habituell kost/annat livsmedel som inte inneh?ller ?ggula, utfallet TMAO i Plasma och studiedesign RCT. Artiklarna skulle vara skrivna p? engelska eller svenska.