The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

If a building is heated by a heat pump, it is of high importance that the heat pump has a high coefficient of performance. An exhaust air heat pump extracts heat from the building?s exhaust air. The air has a relatively high and constant temperature all year around, leading to a high seasonal coefficient of performance. This thesis examines whether it is profitable to complement an exhaust ventilation system with an exhaust air heat pump in an apartment block that already has geothermal heat pump, district heating, oil- or biofuel heated system.

In the middle of Australia there is a city called Alice Springs. The city has one of the highest amounts of daily sunshine hours and sun irradiation in the world. The Australian Government runs a project called Solar Cities, and Alice Springs is one of the participators. The main part of the goal with the Solar Cities project is to increase the amount of electricity generated by solar power and by doing that lower the impact on the environment. This thesis examines the possibilities for construction of a large scale solar power plant in Alice Springs. This is done to determine the potential of creating a more sustainable energy system in the city.

Private state aid enforcement with respect to below market value transactions carried out by Swedish municipalities ? A study on the conformity of Swedish procedural and substantial rules with the principle of effectiveness of EC law Recently in Sweden, several local governmental entities (municipalities) have engaged in commercial transactions that, essentially, have been aimed at transferring the ownership of public services ? for example schools and local medical care centers ? to private undertakings. A few of these transactions have been declared by Swedish administrative courts to constitute illegal below market value transfers of public resources. The essay seeks to determine whether the Swedish national provisions that govern legal claims based on the directly effective Art. 88(3) of the EC Treaty, are compatible with the principle of effectiveness, i.e.

Hammarby Sjöstad is a new district in Stockholm with high environmental standard. Stockholm Water Company evaluates a local wastewater treatment plant with cutting edge technology. The first step is an experimental treatment plant (Sjöstadsverket) with four parallel lines of treatment, each with a capacity to treat wastewater from 150 persons. Within the membrane technology subproject the possibility of using reverse osmosis (usually called RO) to regain nutrients is examined.Reverse osmosis separates the incoming water in a clean permeate and a concentrate that, as the name indicates, is a more concentrated version of the incoming water. Using this method in wastewater treatment, a solution high in nutrients can be obtained without the use of chemical precipitation agent and with no production of sludge.

In connection with the property crises in many countries, caused by the financial crises, people are now more aware of the risks involved in poor property-investments. The unusually cold winters along with extreme prices of electricity in Sweden in recent years has also contributed to increased interest in energy-efficient buildings.The process of developing a modern family home in Kiruna, Sweden is presented the first part of this paper. The house has been designed to withstand very high stresses from snow, low energy consumption as well as a high standard of living comfort. Aspects of the house covered here involves everything from construction, heating and ventilation to sanitation. Each of these areas is presented based on their underlying, scientific methods or theories.

Through analysis of the work of scheduling the doctors at the hospitals of Sundsvall,Gävle and Karolinska in Stockholm this thesis presents some conclusion about howand why a computer system would benefit these very tasks. The reason for doing thisis mainly to save money through more efficient scheduling, but also to minimizeerrors and maximize comfort for scheduler and doctor alike.Scheduling consists of collecting doctors? requests, assessing the need of staff,distribute the available doctors and make sure all tasks are covered throughout thescheduled period of time. The work is often very complex and involves manyvariables ranging from competence to personal whishes and economical constraints.Solving these problems takes a lot of time and energy which could be better spentcuring patients.The conclusion of this thesis is that there is reason to develop such a system to savetime and optimize resources. A description of the system is found in numerous lists ofroles, tasks and prototype sketches which combined shape the content andfunctionality.

A titania-supported nickel catalyst was prepared and tested in methanation in order to evaluate its catalytic properties (activity, selectivity and specially, activity loss), and compare it with an alumina-supported nickel catalyst.The titania-supported catalyst did not only show higher stability than alumina, but also presented a different cause of deactivation, carbon formation. In addition, a kinetic model was obtained for the titania-supported catalyst, and a study of the effect of different operating conditions (temperature, composition and partial pressures of synthesis gas and water) on the deactivation rate and carbon formation of this catalyst was performed.

There's no doubt today that mankind has contributed to the changing climate byher use of fossil fuels. This must change in the nearby future. The Europeanleaders are expressing concern that renewable energy sources are not beingused to their full potential. The 2001/77/EC Directive on Electricity Productionfrom Renewable Energy Sources was accepted in 2001 to encourage thedevelopment of energy production from renewable sources, which isconsidered a step towards the fulfillment of the goal of the Union, that 12% ofthe gross energy consumption should come from renewable energy sources bythe year 2010.The objective of this thesis is to illuminate the common legislation of the EUregarding renewable energy with regard to goal fulfillment, judicial quality,conflicts with other goals of the EU and other faults. The objective is reachedby a hermeneutic study in which interpretation of written text is the mainmethod.

This thesis has been carried out at the Department of Urban and Rural Development at SLU Ultuna during the spring semester 2009. The topic of the paper is how energy consumption can be influenced through design and if the conditions for good design decreases when solutions based on energy efficiency are used. The report is divided into one literature study and one part where energy efficient solutions are used in a design. The thesis work has its origin in a project called "Energihus i Uppsala." The planned building will be a center for energy research in Uppsala. The project aims to demonstrate to the public what is currently happening in research related to energy.

Allmänheten har en stor efterfrågan av produkter inom området för hemautomation. Den senaste bluetoothstandarden Bluetooth Low Energy skapar nya möjligheter för intressanta produkter som underlättar vardagen. Lösningar som IR och Wi-Fi ger inte de förutsättningar som krävs för att på ett energisnålt och praktiskt sätt erbjuda sådana produkter, något som Bluetooth Low Energy gör. I denna rapport diskuteras standarden i syfte att redogöra för hur den kan användas för att automatisera ett hem.För detta examensarbete implementerades en strömbrytarprototyp och en iOS-applikation, vilka användes för att unders öka och påvisa ett koncept för hur tekniken kan tillämpas för hemautomation. Resultaten visar att teknikens räckvidd är dess främsta begränsning.

The aim of the study was to install a biogas system consisting of three tubular polyethylene biogas digesters at a rural household in Malawi and to evaluate the constructed biogas system with regards to relevant system parameters. The purpose was to evaluate if the technology is a possible solution to reduce deforestation and support Malawi in climate change mitigation and adaptation. The study was carried out in the southern part of Malawi in cooperation with the University of Malawi. Locally available materials were used to construct and install the system. Three different feed materials, cow, goat and a mix of cow and goat manure, were used in order to determine which one was best suited to utilise as a digester substrate. After the installation, measurements of temperature, pH, biogas production, biogas composition and system functionality were made. The results of the study show that a biogas system with tubular polyethylene biogas digesters can be successfully installed at a rural household and that the most suitable feed material is a mix of cow and goat manure.

In this essay, we have compared a conventional house to a passive house. The housesshared the same local conditions; they have the same living area and the same layout. Thedifferences between the houses were the different construction solutions for the roof, theouter walls, the floor and the heating system. Both the houses qualify the standards fromBBR and the passive house also lives up to FEBY?s special conditions.The purpose with the essay was to examine if it really is profitable to build small hoses aspassive houses.

The need for snow-free surfaces at squares, bus stops, platforms, car parks and sports facilities increases and at the same time energy price rise and the environment is becoming a hotter issue. The construction of the ground heating systems often lack thermal insulation under the heating pipes, which means that the heat from the pipes not only warm up the soil surface but also heats the surrounding soil. The insulation also has more features, by insulating the risk of frost damage is minimized. The goal of this report is to demonstrate the large heat loss and energy costs in the absence of insulation under the heating system.The report begins with a scientific way to study and treating the soil heating system uses, and the theory involved. Furthermore it also deals with different routing principles of heat pipes depending on the facility.All calculations are made with so called finite element method (FEM), in COMSOL Multiphysics 3,5a, where models are created with different conditions.

An increased awareness about the global warming has created a demand for more information on how the climate is affected by different activities.This master thesis was initiated by Tricorona, a Swedish company that offers its customers analysis and calculation of their climate impact. Tricorona also supplies climate neutralisation with CERs, in accordance with the Kyoto protocol and controlled by the UN. This work demands updated emission factors for greenhouse gases. An emission factor gives information about the greenhouse gasintensity of a service or a product [kg CO2-eq./ functional unit].The purpose of this thesis is to examine how electricity, district heating, hotels, taxis, food and materials affect the climate and how emission factors for these areas can be calculated.This was done by reviewing and comparing different studies and by interviewing experts. The information was evaluated and recommendations on calculations and emission factors were made.The consumption of energy is the main source of greenhouse gas emissions for district heating, electricity, hotels, taxis and materials.