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538 Uppsatser om Gene expression - Sida 7 av 36

The Golden Rule and Bioethics. A Reflection upon the Foundation of Ethics

The object of this thesis is the foundation of ethics. The question is whether there exists a universal core to ethics consisting of a fundamental ethical principle across cultures. This principle could for example be the so-called Golden Rule, which goes as follows: ?You should do to others what you want them to do to you?. The Golden Rule is to be found in many of the world?s religions and is also reflected in secular society.

Sjung för, sjung med och sjung till barn : En studie om hur musicerande situationer förekommer på tre olika förskolora ur ett språkutvecklingsperspektiv

In 1998 years' curriculum for the preschool stands it those children under school age will get possibility that? communicate with the aid of differently expression shapes? and an example that is mentioned in the governing document is song and musical.  The purpose with this study is to examine how the song and the music are used from a language development perspective in three different preschools' activities. In the essay, I review to earlier research around the music?s' and the song's possibilities for the child's linguistic development. In the essay, it is discussed about some important factors that obstruct music situations in the preschool that the curriculum for the preschool (Lpfö98) not specific describes what expression shapes as song and music will contain and that deficiency on musical education at pedagogues.  The method for the study was to collect empirical materials through observations on three different preschools.

Yttre gestaltning för upplevelse inifrån

The report aims to show how outdoor environments have been shaped with the purpose of experiencing them from the inside of buildings, through an overview perspective. Two Danish gardens are used as examples. One is the Baroque garden in the garden of Frederiksborg castle in Hillerød. The Baroque garden is thus a part of a larger garden. The second garden is the Charlotte Garden, a relatively newly constructed garden within a housing complex in Østerbro in Copenhagen. Both gardens are analyzed on the basis of visual expression and structure. In the report, it is also discussed how the contemporary society have evoked the different ways of shaping the gardens.

Samspelet mellan grafisk design och ljusdesign i utställningssalen "Vägen till det förflutna"

The purpose of this study was to develop recommendations for using more efficient lighting and better graphic design to create a more uniform structure in the exhibition hall ?Road to the past? in Jönköping County Museum by answering the following questions at issue:1) How are the objects in the exhibition hall ?Road to the past? exposed today?2) How can you use lighting and graphic design to create structure in the exhibition and strengthen the expression in the exhibition?Our work is based on observations and open group interviews. We observed the exhibition hall ?Road to the past? during day and evening. In the same hall, we observed a guided tour that we followed up with open group interviews.

De allra minstas språkutveckling i förskolan : Pedagogers syn och estetikens betydelse

The study has been conducted to find out whether aesthetic expressions and experiences can benefit the smallest children's language development. How do the teachers at the preschool relate to aesthetic expression? Do they think that aesthetic expression and experience will be favorable for stimulating children's language? Children perceive through their senses, logically aesthetic expressions should promote children's learning and development. How does this claim stand in relation with the smallest children's language development and teachers' views on aesthetics as a pedagogical tool for language stimulation? To get closer to the answer a survey have been done.

Bestämning av FTO (Fat mass and obesity associated gene) polymorfism

Vetenskapen har på senare år försökt fastställa de olika orsaker som leder till fetma. Det är känt att högt energiintag och för lite motion för eller senare hos de flesta individer resulterar i fetma. Det som kan konstateras är att ärftlighet i samspel med miljön vi lever i och påverkas av kan vara den huvudsakliga orsaken till en rad sjukdomar inklusive fetma. På senare år har forskare upptäckt olika gener som på ett eller annan sett är involverade i ämnesomsättningen. En sådan gen är ?fat mass and obesity associated gene?, FTO.

Dialog, maktrelationer och våld : En kvalitativ studie om maktrelationer i klassrummet och våld som uttryck för motstånd

Violence against teachers in Swedish schools, according to recent reports has increased and there have been many studies to investigate the situation for teachers. The surveys show that teachers are especially vulnerable to students. This study aims to examine the relationship between a number of teachers and their students to study the power relations that exist in the classroom. This is to see what violence is an expression of and also how violence is perceived by the teachers. The issues that are central in this study are:What is the importance of dialogue in the relationship between these teachers and their students?Is there power relations between teachers and their students?What is the violence against teachers and expression of?Is there any connection between dialogue, power relations and violence?The theories of the materials in this study was analyzed using power relations theories of the historian of ideas Michel Foucault and Philosophy Doctor Anders Persson, who to some extent has his theoretical basis in Foucault?s power relations.

Hedersrelaterat våld - Hur kan hedersrelaterat våld förklaras, och hur används begreppet i svensk media?

The purpose of this study has been to explore definitions of violence performed in the name of honor, and the conditions under which the phenomenon occurs and is upheld. Furthermore, the study aimed at exploring how the Swedish expression for violence in the name of honor, ?hedersrelaterat våld?, is used within the Swedish media. To achieve these purposes, previous research in this matter was reviewed, as well as an analysis by means of categorizing the content of news articles from two Swedish daily newspapers. Previous research has showed that this type of violence occurs foremost within cultures upholding patriarchal structures and traditions, and women?s (non-)sexuality is looked upon as being of an utmost importance for the family?s honor.

Känsloigenkänning i form av ansiktsuttryck med Kinect

Facial expressions are a part of our body language that helps us to clarify the verbal communication between humans. We use our facial expressions every day, both consciously and unconsciously, to express emotions and attitudes depending on the situation. The hypothesis of the study is: Given a facial expression, how well can Microsoft Kinect, as an input method, determine a person?s feelings with the two algorithms Naive Bayes and Sequential Minimal Optimization? The feelings are limited to happy, sad, surprised and disgusted. With the help of Kinect, a person?s facial data, both coordinates of the face and parameterized data, were saved and used for machine learning.

Grb10 and developmental programming: evaluation of a maternal diet restriction model during gestation

This study was conducted at the University of Bath, UK, from September to December 2014 as part of an ongoing research project aimed at elucidating how the Grb10 gene might act as a mediator of long-term health effects (such as predisposition to obesity, diabetes, coronary heart disease and hypertension) caused by environmental factors during development. This phenomenon is known as developmental programming. The purpose of this thesis was not primarily to answer any of the broader questions posed by this research project at large since this would require much more data than is reasonable to acquire over the course of a few months, but rather to evaluate the methods used in this project and reveal whether they are working the way they are presumed to. In the research project, which employs mice with the Grb10 gene knocked out as well as wild-type control mice, a dietary restriction model is utilized during gestation, which is supposed to generate offspring with lower birth weight and subsequent detrimental health effects in adulthood. This is supposed to be achieved by restricting the protein content of the pregnant mothers? diet to 9 % (as opposed to the control diet of 20 %) throughout gestation.

Genetic variation of ASIP and MC1R in past and present sheep of Gotland

Ovine black coat colour is determined by the MC1R (Melanocortin 1 receptor) and ASIP (Agouti signalling peptide) genes at the Agouti and Extension loci. The black phenotype is caused by production of eumelanin by melanocytes, whereas yellow-tan or light phenotype is the result of phaeomelanin synthesis. The ovine MC1R gene has two known alleles: the wildtype (E+) and the dominant black (ED) alleles. Two missense mutations (c.218T>A and c.361G>A) constitute ED. The presence of a third allele, the e allele, is proposed and believed to give rise to phaeomelanic phenotype.

Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil

Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence assay, mouse inoculation and RT-PCR. The sources of infection were determined by sequencing 234 nucleotides of the 5? end of the N-gene and align these with homologous sequences retrieved from GenBank.

Expression av PSI-N från Arabidopsis thaliana i E : coli

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

Expression av PSI-N från Arabidopsis thaliana i E.coli

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

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