Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil
Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection
for all animals and humans are bites from dogs or bats. The aim of this degree project was to
diagnose and determine the source of infection for 11 rabies samples from São Paulo State
and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence
assay, mouse inoculation and RT-PCR. The sources of infection were determined by
sequencing 234 nucleotides of the 5? end of the N-gene and align these with homologous
sequences retrieved from GenBank. All seven samples for which satisfactory sequences could
be achieved clustered with the sequences related to vampire bat with a bootstrap value of
98%.