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124 Uppsatser om Fluorescence in situ sequencing - Sida 7 av 9

Infectious bronchitis in Brazil : a minor field study in Bastos municipality, Sao Paulo

Infectious Bronchitis (IB) is a highly contagious viral respiratory and productiondepressant disease distributed world wide. The disease is caused by the InfectiousBronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within thegenus Gammacoronavirus. Due to a combination of high mutation rate and atendency of recombination a constant emergence of new genotypes and serotypesis seen. In Brazil, a country with an extensive poultry production and majorproblems with disease control, the diversity of circulating strains are wide andoutbreaks of clinical disease despite intensive use of vaccines are common. Theaim of this study was to investigate the presence of IBV and to identify genotypesof isolated IBVs in a limited number of layer flocks in Bastos, São Paulo, as partof the screening for circulating genotypes in the country.

Bedömning av oralmotorik och talmotorik med VMPAC hos typiskt utvecklade barn, 4-10 år

Syftet med denna studie är att undersöka hur en grupp typiskt utvecklade, svenska barn, i åldrarna fyra till tio år presterar på tre deltest i VMPAC, för att därmed skapa ett underlag för en referens för svenska barn i bedömningen av avvikande utveckling. Studiens frågeställningar är: 1. Hur presterar en grupp typiskt utvecklade, svenska barn, i åldrarna fyra till tio år, på tre deltest i VMPAC (Focal Oromotor Control, Sequencing Maintenance Control och Connected Speech and Language Control)? och 2. Förekommer det några skillnader mellan en grupp typiskt utvecklade, svenska barn, jämfört med den amerikanska normeringen i VMPAC och barn med verbal dyspraxi (CAS) undersökta inom ramen för en magisteruppsats (Björelius-Hort, 2009)? Oral- och talmotorisk förmåga hos 23 svenska barn i åldrarna fyra till tio år, som åldersmatchats mot barn med verbal dyspraxi (Björelius-Hort, 2009), testades med deltest två, tre och fyra ur VMPAC.

A survey of cassava plants in the coastal region of Tanzania showing severe symptoms of cassava mosaic disease

Cassava (Manihot esculenta Crantz) is a root crop that plays an important role in many households in Africa. It is sometimes called ?Africa´s food insurance?. The advantages with the crop are that cassava is drought-resistant and it can grow in a semi-dry land. It is a perennial crop and does not require much labor.

Förekomst av tenn och koppar på bottenmålade fritidsbåtar 2011

Det har uppmärksammat att det fortfarande förekommer tenn och koppar från båtbottenfärg på fritidsbåtars skrov trots förbud av båtbottenfärger med tennföreningen TBT sedan 1989 och reglering av kopparbaserade färger sedan 1999.Syftet med denna kandidatuppsats är att ge en dagslägesrapport vad gäller förekomst av tenn och koppar på fritidsbåtars skrov.Under våren 2011 har tester på 336 fritidsbåtar i tre vattenområden gjorts med hjälp av en handhållen XRF (X-Ray Fluorescence, röntgenfluorescens) som genom avläsning av en yta visar halter av olika grundämnen. Testerna utfördes på vinterförvarade fritidsbåtar <12 m hos båtklubbar vid Mälaren, Storsjön och Härnösandskusten. Undersökningen visar att 73 % av båtarna i denna undersökning har halter av tenn och/eller koppar överstigande >2500 ppm, vilket är det för rapporten satta riktvärdet. Det innebär att de skulle kunna tas vidare till kemisk testning med till exempel gaskromatografi kopplad till masspektrometri (GC-MS) för att avgöra exakt vilken substans färgen innehåller. Resultatet visar också att användningen av tennfärger antingen fortfarande förekommer i liten skala eller att gamla tennfärger är så pass starka att de ger utslag genom övermålade färglager.

Tillbyggnad vid Nationalmuseum : Parametrisk studie

Tillbyggnad NationalmuseumKontext och formJag ville förhålla mig fritt i utformningen av en tillbyggnad till Nationalmuseum. Muséet är pampigt och behöver kontraster snarare än likhet.Platsen utmärker sig dels i att den är nästan plan och att den är kraftigt skuggad av NM och Lydmar hotell under framförallt vintermånaderna. Tomten har även stora träd som stäcker sig över NM och ger platsen uttryck.Så jag ville arbeta vidare parametriskt med dessa platsbundna element.  Det första steget var att definiera en volymgräns som samspelar med platsens träd och byggnader. Även att skapa positiva uterum för människor. Solen bör ha tillgång till alla husets inre delar, och detta skapar smala volymer.

Variation in frequency of alleles in the MTNR1A gene with possible impact on ability of ewes to show oestrus out of season

In Swedish sheep industry it is desired to have lambs slaughtered evenly distributed over the year. Swedish sheep are generally seasonal breeders with lambs born in the spring and slaughtered in the autumn. Some of the breeds, e.g. Swedish Finewool and Roslag sheep breeds have shown ability to lamb out of season. For increased knowledge on the possibilities to breed for out-of-season lambing in Swedish sheep breeds, the aim of this master thesis is to study the variation in frequency of two alleles in the MTNR1A gene in the three breeds; Gotland, Swedish Finewool, and Roslag sheep.

Utlakning av tungmetaller från Skörby deponi

The purpose of this thesis has been to assess whether leaching from one particular Swedishlandfill site, Skörbytippen, is contributing to elevated levels of heavy metals in a ditch runningfrom the landfill site to the lake Mälaren in the communal region of Håbo kommun. Since theditch is stretching approximately five km downstream from the landfill site, in situ observationsand maps with known points of inflow was utilized to determine appropriate test points for thecollection of samples.Soil, water and sediment samples were collected and analyzed for content of organic material, pHand conductivity. Data on plant-available elements and total amounts of heavy metals wasacquired through ICP-analysis.The results show that the Skörby landfill is currently in the methane-forming anaerobedevelopment phase, which indicates that the concentration of free and complex-bound metals inthe leachate water should be low due to chemical binding with other landfill compounds. The pHof the sediment samples was generally one unit higher than the soil samples and some of themeasured data, such as pH and conductivity, show that an environmentally disturbing dischargeof some kind of strongly alkaline chemical(s) has been made from Benders? takpanneindustri inclose proximity to the landfill.

Metallers lakningsbenägenhet hos järnsand i banvall

Detta examensarbete har utförts åt Banverket Norra Banregionen, Investeringssektionen i Luleå. Syftet med arbetet var att med hjälp av lakförsök undersöka metallers lakningsbenägenhet och miljöpåverkan hos banvallsmaterial som innehåller järnsand. Banvallarna kan vara en källa till spridning av föroreningar. De innehåller föroreningar som t ex rester från oljor, tungmetaller, bekämpningsmedel och impregneringsmedel. I banvallarna har olika typer av slagg bl.a.

Jämförelse mellan en träbro och en betongbro : En LCA-analys med fokus på koldioxidutsläpp samt en LCC-analys

This report is a thesis for collegeengineering program in structuralengineering at Uppsala University andwas commissioned by the Swedishcontractor Svevia. The study aims toprovide an overview of the mostbeneficial bridge material from bothenvironmental and economic point ofview.The life-cycle analysis covers onlycarbon dioxide emissions, since it isthe most spoken of in today?s climateagenda. Furthermore, this study aims tohighlight which parts, of the systemboundary, that contributes to greatercarbon emissions for the wood- and theconcrete bridge.The life-cycle cost analysis wascalculated with a lifespan of 40 yearsand covers the basic cost of investment,operating and maintenance, anddecommissioning costs.The analyzed constructions in this studyare existing projects that are almostcompleted. Both bridges were designed tohandle loads up to 60 tons.

Rotningsstimulerande effekt och kemisk analys av vattenbaserad extraktion av vedartade och örtartade skott av Salix smithiana Willd. :

Cuttings of Lonicera xylosteum L. were treated with willow extract to study the response in rooting capacity. The extracts were prepared by mixing chopped pieces of Salix x smithiana Willd. with water in a warring blender. The investigation comprised 2 types of salix extracts based on differences and dynamics between twigs in winter dormancy and twigs with new shoots. To explore the rooting response, three tests were made.

Species diversity and geographical distribution of Fusarium species on winter wheat in two regions in Sweden

Various Fusarium species causes some of the most common cereal diseases worldwide. Besides the yield losses that can be a result of these diseases, strains from several Fusarium species can produce mycotoxins, some very toxic. The aim of this study was to investigate which Fusarium species and how many that occurred in winter wheat grains from Mälardalen and Kalmar län, if there was any difference in the distribution of Fusarium fungi between the regions and the potential within species to produce mycotoxins belonging to the group trichothecenes. Winter wheat grains collected in 2009 from ten fields (unsprayed plots) in the two regions were analysed for Fusarium species. PCR was used to amplify the TEF region where after the samples were sequenced.

Tollarsjuka hos Nova Scotia Duck Tolling Retriever : en sjukdomsbeskrivning

In the dog breed Nova Scotia Duck Tolling Retriever (NSDTR) several dogs suffer from joint pain and possibly pain from the muscles. This diffuse disease is called Immune-mediated Non- Erosive Arthritis in Nova Scotia Duck Tolling Retriever. The disease is most likely of autoimmune nature where the etiology still is unknown. The musculoskeletal symptoms of stiffness and shifting lameness are intermittently seen. Some dogs also have dermatological changes and some dogs suffer from pain when opening their mouth. The study included 20 diseased and 20 healthy NSDTR. The purpose was to describe immune-mediated arthritis in NSDTR based on symptoms, results of analysis and effect of treatment. Among the diseased dogs 65% had serum positive for antinuclear antibodies by indirect immunofluorescense (IIF) ANA-test.

Konsolidering av v?vsp?nda papperstapeter: analys och metodutveckling

Printed and painted wallpaper on a weaved fabric backing was a common way to decorate homes in Sweden during the eighteenth and nineteenth centuries. Not much is written about the craftsmanship in relation to the hanging of these wallpapers and even less about the conservation of the objects. Even so, many wall segments remain in situ and in museums and are more often than not in dire need of conservation such as deacidification and consolidation. These are composite objects, consisting of cellulose materials with different properties along with adhesives and paints. Analysis of the materials is required to ensure safe handling and choice of conservation materials, and to establish a treatment plan which will be executed in several steps. This thesis aims to identify these steps and develop a method for conserving the wall segments with suitable materials, preferably without altering the source material to any significant degree.

Survey of Fusarium species on yellow onion (Allium cepa) on Öland

It has been observed by both onion producers and a plant protection advisor on Öland (an island off the east coast of Sweden) that basal rot is the largest contributory factor to reduced onion quality and yield. Basal rot is mainly caused by species of Fusarium fungi. The aim of this study was to: a) investigate which species of Fusarium that can be found in onion produced on Öland, b) describe the symptoms caused by the different Fusarium fungi found and c) explore, through interviews with the onion producers on Öland, the mechanisms that may be involved in the observed increase in basal rot.Onion bulbs (Allium cepa) were sampled on two occasions. In total 181 onions from 11 different fields were analysed. In addition, eight onion producers were interviewed.

Molekylärgenetisk analys av dilaterad cardiomyopati hos hund :

Canine dilated cardiomyopathy (DCM) is a disease with high morbidity and mortality and with a high prevalence in certain breeds. Predominatly large breeds are affected, such as Great danes, Newfoundlands, Deerhounds and Dobermanns. One exception is Cocker spaniels, where both American and English Cocker spaniels are affected in a quite high frequency. Cardiomyopathy is characterised as a disease that affects the myocardium and gives an impaired heart function. To diagnose DCM following criteria have to be observed: 1. Dilation of the left ventricle. 2. Reduced systolic heart function. 3. Increased sphericity of the left ventricle. To be certain of the diagnosis, alternative reasons for the symptoms such as lung- and heart diseases with other etiologies have to be excluded Two different types of DCM have been found upon histological examination.

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