Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

AbstractThe purpose of this bachelor thesis is to describe the use of artificial insemination (AI) in sheep and discuss the possibilities for AI in Sweden. Male and female fertility, heat detection, semen handling and insemination techniques in sheep are described. Advantages and disadvantages of AI are discussed. In order to achieve genetic progress it is important with well planned and implemented breeding programs. The Swedish Sheep Recording Scheme and computer software from Elitlamm are the basis for sheep breeding in Sweden.

The global Positioning System (GPS) provides location and time information as long as there are unobstructed lines of sight to four or more GPS satellites. However, when this is not the case the signal may be inaccurate or sometimes even completely blocked. In these situations the Inertial Navigation System (INS) is an appropriate choice for positioning.  An INS has already been proposed in a previous thesis by Erik Andersson and the objective of this thesis is to fuse the GPS with the INS in a proper way. A part of this project is to decide the reliability of the GPS.Three methods for GPS reliability detection have been proposed. One method based on the statistical properties of each of the separate systems, and two methods based on the statistical properties of the residuals between the GPS and INS.

For some time researcher has focused their interest on how women are being treated in sports and what impact it has on the development of the sports movement. Researchers in the field have tried to clarify where the problem lies, if it is gender or sex. Are the differences between the sexes caused by how society portrays women or because women and men are built for different purposes. This research paper studied how women in the sports of snowboarding and freeskiing perceived their possibilities to practice their own sports. Furthermore, the study tried to answer if the problems are originated from the culture of the sports, what the possibilities and opportunities women had to make ?it? and what women think is the reason why there are so few female athletes in their sports.

The purpose of this study is to research into the role of the citizen and the educational system in Sweden throughout periods of time based on previous theoretic works, and also to deepen the understanding of students with special needs in Swedish education focusing on the primary school years. This shall be carried out by illustrating the different points of view and choices within the educational system that are at hand for students with concentration difficulties and social difficulties.I will analyze these points through a cultural and social perspective on contemporary society in order to contribute towards today?s discourse on the norm. The result of this empirical investigation presents a dilemma existing in the democratic educational system of today. Nationally and internationally, the educational system has been structured around a normative system containing both rights and duties that effect everyone working within it.

A large part of the agricultural products that are sold in Sweden are imported because Sweden cannot meet the consumers agriculture products demand all year around. One problem that can occur and become problematic when trading across boarders is if there are errors in the delivered agricultural products. What must the Swedish buyer do, for example, make for it to be considered complained fault in the goods against the seller? Another problem in international trade is that the buyer may need to apply complaint rules they have not previously been in contact with since their domestic law is not always applicable to the contract. To avoid getting into this situation, the buyer should try to negotiate with the seller that the contracting parties shall use compensation claim rules that the buyer is familiar with, or which is even more advantageous than to the seller.

A power system will inevitably be subjected to transient overvoltages, most often produced by switching operations and lightning strikes. These transient overvoltages may harm sensitive equipment without sufficient protection, but it is not an easy task to predict what transients may occur or how they affect the system. A fault occurred in a low voltage system at Forsmark 2012 and overvoltages from lightning were concluded as the probable cause. The three aims with this thesis are to develop and test a model of the power system at Forsmark 3, analyse the transient behaviour of the system when subjected to lightning surges and identify critical parameters, and lastly to provide a tool for investigating the protection of the system. The modelling and simulations were performed in the freely available simulation program LT Spice.

I denna rapport presenteras ett tillvägagångssätt för att hitta och datera brytpunkter i tidsserier. En brytpunkt definieras av det datum då det skett en stor nivåförändring i tidsserien. Det presenteras även en strategi för att skatta effekten av daterade brytpunkter. Genom att analysera tidsserier över AFA Försäkrings ärendeinflöde visar det sig att brytpunkter i tidsserien sammanfaller med exogena händelser som kan ha orsakat dessa brytpunkter, till exempel villkors- eller lagförändringar inom försäkringsbranschen. Rapporten visar att det genom ett metodiskt angreppssätt går att skatta effekten av en exogen händelse.

The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

Two species of bees in the genus Apis, real honey bees, has long been of interest for man. These two are the European honey bee, Apis mellifera, and the Asian honey bee, Apis cerana. In Vietnam, beekeeping is of great importance, both with A.cerana and A.mellifera. The aim of this project was to investigate if the introduction of the European honey bee in Asia has affected the Asian honey bee, and whether different pathogens from A.mellifera have been transferred to A.cerana. Totally 40 samples, 20 from every species, were analysed for 8 different viruses.

Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine.

The aim of this essay was to study how the communities work with groups as a support to children with addictive parents, and to increase my understanding of the groups meaning as a support. My main questions have been: How are the child group sessions formed? What kind of experience does the group leaders have regarding their functions? The study is a qualitative investigation that has been turned to prefessionals in the subject area and  is based on 6 interviews and secondary sources. The material has been analyzed from a narrativ theory as well as a salutogent perspective. Research shows that children growing up in an addictive environment often have decreased psychological health and are overrepresented amongst those who developed a future addiction.

In today?s horse keeping, there is an increasing awareness of the importance of movement and activity for the horses comfort and tenability, while tradition has it that horses should be kept in boxes and small paddocks. In order to develop housing systems that fulfils and optimizes the horses needs regarding movement and activity, we need to know more about factors that influence the activity patterns of horses. A first step is to be able to record and analyze the gaits in horses with an automated system. This could be done by use of accelerometer data.In this pilot study, existing accelerometer technique was used to characterize the different gaits in horses (walk, trot and canter) by studying how their respective signal patterns differ and how this is influenced by the size of the horse.The accelerometers being used in this study measures the movement in three different spaces; horizontal, lateral and vertical.

The purpose of this project is to analyze earthing systems for houses. By practical measurements and theoretical analyzes consequences of possible faults, that can occur within the facility and in the low-voltage distribution network, is illustrated.  The reason for this project is that nowadays one has to arrange with a main equipotential bonding system for buildings. Apart from that, if necessary, bathrooms must be provided with a supplementary equipotential bonding system.The practical measurements verify that potential differences in facilities may be due to voltage drops in the PEN conductor between the origin of the electrical installation and the power transformer.Based on the theoretical analyzes of possible faults, it is considered that the requirement for  supplementary equipotential bonding systems for bathrooms in most cases can be questioned, when basic insulation, fault protection, residual current devise and main equipotential bonding system already provides an adequate level of protection.A PEN conductor loss may result in a situation where metallic parts attached to the equipotential bonding system starts working as earth electrodes. Equipotential bonding system may also increase the incidence of stray currents. If TN-C-S systems is provided with a local connection to earth a redundant system against PEN conductor loss is obtained.

Nuts of all kinds are common ingredients in food. For nut allergy sufferers the frequent use of nuts cause problems and "hidden" nuts in food products may elicit allergic reaction when such foods are consumed. Methods for detecting and quantifying walnut (and other nuts) with high sensitivity and specificity are therefore very important.The objective of this project was to verify the specificity of a rabbit antiserum against walnut with immunodiffusion and to determine the size of the dominant walnut antigens with Western blotting. In addition, a commercial sandwich ELISA for walnut quantification was validated and compared with a qualitative real-time PCR.The rabbit antiserum proved to be less specific but after absorption with cross-reacting nuts and seeds it showed high specificity. The ELISA kit reacted, except for walnut, with pecan and slightly with other nuts and seeds tested.