Abstract Tripeptidyl-peptidase II (TPPII), is present in most eukaryotic cells. It cuts tripeptides from the N-terminus of peptides and is especially important for degrading peptides longer than 15 amino acids. TPPII also tailors long peptides into suitable substrates for the Enzymes which transport and produce the peptides that MHC I present. Increased levels of TPPII have also been found in certain cancer cells, thus it is of interest to determine if TPPII could be used as a tumour marker.The aim of this study was to optimise and evaluate 3 different methods for quantifying TPPII. Western blot, Enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions.

Pyometra, chronic purulent metritis in metoestrus, is a common disease in intact bitches. About 25% of all bitches in Sweden have contracted the disease before ten years of age. Clinical signs of the disease are varied, and the course may be either slow or acute. The diagnosis is most commonly made by radiographical or ultrasonographical examination of the abdomen in combination with blood biochemical and hematological analyses. The safest and most efficient treatment of pyometra is surgical ovariohysterectomy.There is room for improvement of both diagnostic tools and possible prognostic indicators for pyometra.Mast cells are large leucocytes, with granule that contain inflammatory mediators.

An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined for potential errors. Fatal errors were pinpointed to the ligation reaction and the transformation required to generate and propagate the desired vector pETGH-(target)KO.

In Uganda, small scale pig production plays a major role for households to earn a living and to secure their access to meat. Piglet mortality due to diar-rhoea-related dieseases is high. In an attempt to understand the underlying causes for this high mortality this study aims to investigate the role of trans-missible gastroenteritis virus (TGEV) and is a part of a larger project con-ducted at Makerere university in Uganda. A complicating factor in this investigation is the porcine respiratory corona-virus (PRCV), a stabile mutant form of the TGE virus. The occurrence of this virus had shown to alter the impact of TGEV in e.g. Europe and North Amer-ica.

Oxochlorates are anions with a partially naturally occurrence in nature but are also spread by human activities, including the paper industry. These compounds are harmful to both nature and humans, which makes it necessary to find a good way for their degradation. There are two different kinds of bacteria that can use oxochlorates as electron acceptors in their metabolism, bacteria that break down perchlorate and bacteria that break down both perchlorate and chlorate. A bacterium that can break down chlorate under anaerobic conditions is Ideonella dechloratans which holds the genes for chlorite dismutase and chlorate reductase which are Enzymes for the degradation of chlorate. Gene expression and Enzyme activity of chlorite dismutase are induced under anaerobic conditions, which makes it interesting to find out how this regulation functions in order to better exploit these bacteria in biological wastewater treatment.

Human T-lymfotropt virus (HTLV) är ett C-typ onkovirus som tillhör familjen Retroviridae som huvudsakligen angriper T-lymfocyter och orsakar leukemi och andra autoimmuna sjukdomar. I den nuvarande kliniska diagnostiken används Enzyme-linked immunosorbent assay och ibland vid screeningsmetoden uppträder ospecifika reaktioner. På senare tid har flera metoder baserade på real-tids PCR utvecklats för att bestämma halten av virala genom i patienter. Syftet med denna studie var att sätta upp och optimera en realtids-PCR för detektion av humant T-lymfotropt virus typ-1 och 2. Inför optimering av realtids-PCR extraherades DNA från MT-4 och MO-T cellinjer.  Under optimering av realtids-PCR användes SYBR Green och smältpunktsanalys där flera komponenter bl.a.

Thymidine kinase 1 (TK1) is an intracellular Enzyme involved in ?the salvage pathway? where the uncomplete parts of DNA are transformed into DNA precursors. The expression of TK1 is cell cykle specific, with the highest level during S-phase when DNA-synthesis is most active. One kind of TK1 is present in serum and an increase in TK1 activity in serum is due to either the level of DNA synthesis in the body or the number of cells dying in a state of replication. This can be used and is used as a marker for prognosis and evaluation of treatment in human patients with leukemia and lymphoma.

Cytokiner eller interleukiner är signalpeptider med låg molekylär vikt som reglerar många viktiga funktioner. De kan delas in i två grupper beroende på deras effekt på celler. Interleukin 4 (IL-4) till exempel kan tillhöra gruppen tillväxtfaktorer medan interleukin 6 (IL-6) kan tillhöra gruppen aktiverings- eller differentieringsfaktorer. IgM-receptorn eller B-cellsreceptorn, BCR, finns på Bceller och är membranbundna immunoglobuliner (mIg) som har två huvuduppgifter; att förmedla signaler som styr B-cellens utveckling samt att binda in antigen som sedan ska presenteras för T-celler. I studien aktiverades B-celler med antikropp mot IgM (anti-IgM) samt rekombinant IL-4.

The guinea pig is an important meat-producing animal in Peru as well as in several other South American countries. They are held in different kinds of systems, from the small backyard breeders with few animals to the large commercial farms with thousands of guinea pigs. One of the major issues at the farms is animals dying of unknown causes. Salmonellosis is often considered as a major cause, but samples for confirmation are rarely taken. It has been known for a long time that Clostridium difficile (C.

Alzheimers sjukdom (AD) är den vanligaste formen av demens och kännetecknas av intracellulärt neurofibrillärt trassel (NFT) bestående av proteinet tau och extracellulära plack, uppbyggda av peptiden amyloid beta (A?). En växande skara studier har börjat peka mot att AD är en hjärnspecifik typ av diabetes. Insulinresistens följt av hyperinsulinemi och hyperglykemi är kännetecken för diabetes mellitus typ 2 (DMT2) och har visat sig vara en riskfaktor för AD. Insulin, ett hormon som kontrollerar glukoshomeostasen i perifera nervsystemet (PNS) och är viktigt för minne och inlärning, transporteras över blod-hjärnbarriären i en mättnadsbar transportmekanism och dess koncentration i centrala nervsystemet (CNS) minskar vid DMT2 och AD.

The increased frequency of reported Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of great concern to small animal healthcare due to limited options of antimicrobial therapy. The aim of this literature study is to investigate 1) characteristics and classification of S. pseudintermedius, 2) resistance mechanisms and emergence of MRSP, 3) risk factors for selection of MRSP, and 4) clonal distribution around the world. S. pseudintermedius is a skin and mucosal commensal in healthy dogs and cats, but can cause infections, including pyoderma. Beta-lactam antibiotics inhibit bacterial growth by binding to the penicillin binding protein (PBP), a vital Enzyme in the synthesis of the bacterial cell wall.

Grewia optiva är en utav många outnyttjade stjälkfibrer som skulle kunna bidra till ökandet utav de naturliga fiberalternativen. Fibern har idag inte så många användningsområden på grund utav dess hårda och styva uppbyggnad, vilket gör den svår att spinna till garn. På uppdrag av organisationen Bhartiya Gramotthan Sanstha (BGS) har i detta projekt en redan befintlig metod utvecklats för att förädla fibern. Vad som främst eftersöktes var nedbrytandet av pektin som är en av de faktorer som bidrar till fiberns hårda och styva struktur. I metoden användes biologiskt nedbrytbara enzym som katalysatorer.

Bakgrund: Deposition av fibrillär amyloid beta 1-42 (A?) i hjärnan är ett välkänt kännetecken för den neurodegenerativa sjukdomen Alzheimer?s (AD). Dessa ansamlingar påverkar pericyter, en celltyp involverad i blodkärlsfunktion och upprätthållande av blodhjärnbarriären (BBB). Pericyter uttrycker både receptorn low density lipoprotein receptor related protein 1 (LRP-1) till vilken A?1-42 binder, och proteoglykanet NG2.

Ticks and the diseases they transmit are of major importance throughout the world. In Uganda, cattle are the most important livestock from an economic point of view. Livestock keepers fear bi-directional transmission of tick-borne pathogens between their livestock and wild animals. This cross-sectional study was conducted to establish and compare the sero-prevalence of the tick-borne pathogen Babesia bigemina among randomly selected Ankole Long-horned cattle and European crossbred cattle on 30 farms in Kiruhura district, in two sub-counties near Lake Mburo National Park in South-western Uganda. Half of the farms were situated in close proximity to the park and thereby housed cattle with more frequent wildlife-livestock interface (Sanga), whereas the other half had less frequent contact (Kikatsi).

Background:  Heart failure is a multidimensional phenomenon with high mortality. Heart failure is treated with angiotensin converting Enzyme (ACE) - inhibitors or angiotensin receptor blockers (ARBs) that counteract neurohormonal stimuli that occur in heart failure, as well as providing vessel dilatation, which reduces symptoms and the need for hospitalization and increases survival. Despite this, only about 50% of heart failure patients survive 6 years after diagnosis with drug therapy, and as heart failure is increasing globally, due to improved care and treatment and increasing life expectancy of the population, there is a great need for new drugs such as LCZ696 that acts by dual inhibition of the renin - angiotensin - aldosterone system and neprilysin inhibition.Objective: The aim of this literature study was to evaluate the efficacy of current treatment and possible future treatments on mortality and morbidity in heart failure patients.Results: The examined articles show that treatment with ACE inhibitors in patients with symptomatic heart failure reduces the risk of total mortality by 16% over 3.5 years, reduces all-cause mortality or hospitalization due to heart failure with NNT (number needed to treat) = 10.4 over 3.5 years and increases median survival by 9.2 months over 12.1 years in patients with asymptomatic heart failure. Treatments with high-dose ACE inhibitors reduce mortality and hospitalization because of cardiovascular causes and hospitalizations from any cause by NNT = 30 over 3 years. Beta-blockers reduce sudden death and total mortality and cardiac death or non - fatal myocardial infarction with NNT = 38 and NNT = 23, respectively, over 12 months.