Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

Abstract The purpose of writing this thesis was to improve methods during requirements engineering phase. Usercentred system engineering has some problem areas, which are examined and verified to create a new guideline for developers. This guideline tends to make requirements engineering more effective and help developers create more concrete requirements. It is not uncommon that system development projects ends up with unsatisfied users or delay in deliveries. The reasons are different kinds of communication problems between users and developers during verification of requirements.

I detta examensarbete presenteras olika inriktningar av musikterapi och FMT-metoden (Funktionsinriktad musikterapi) belyses ingående. Här beskrivs arbetssätt och arbetsmaterialet nämns. Ett historiskt perspektiv ges både på människor med funktionshinder genom tiderna och på musikterapins utveckling i världen och i Sverige. Olika musikterapidiscipliner presenteras liksom befintliga utbildningsmöjligheter. Två elever/adepter presenteras och terapin med dessa beskrivs detaljerat. Arbetet tar upp frågan om FMT-metoden måste ändras eller justeras för att kunna användas i terapin med en synskadad person? Kontentan är att det inte behövs några förändringar av metoden för att fungera för adepter med synskada..

The aims of the present study were to implement and evaluate a three-session Acceptance and Commitment Therapy (ACT) stress management intervention in a non-clinical group of university students (N = 25) and to investigate the importance of practice in an ACT-intervention. A design with pre-test and double post-test was chosen. A comparison was made between a nine-hour ACT-intervention containing practice for the participants, a two-hour lecture on ACT and stress management containing no practice for the participants and a control group. Psychological flexibility was assessed with Acceptance and Action Questionnaire-2 and mindfulness was measured with Kentucky Inventory of Mindfulness Skills. Perceived stress level was measured with Perceived Stress Scale.

Globalization has among other tendencies led to the emergence of new ways of doing business. One of the ways for organizations to meet the demands of a new competitive landscape has been through management accounting change, a field which has become increasingly popular in research. This paper builds on that research and seeks to investigate the case of the implementation of a new management accounting system in a Swedish company which was triggered by strategic change. Commonality through the interactive approach is by some researchers seen as a key to successful change. This approach is contrasted to the importance of symbolic constructs and change recipients? cognitive effort to understand the meaning of change for which Framing theory is applied.

This study is about how to develop younger teens problem solving ability and their social competence. The study carried through by using a qualitative method, two observations and ten interviews. The observations where preparatory for the structured interviews. The respondents where pupils in a class were it is an ongoing project which purpose is to develop childrens problem solving ability and social skills with cognitive training and an empowerment based reasoning model that is called rahyab. Rahyab is a Persian word and it means path- finding.

This paper examines the impact of conscious shelf management on online grocery stores. An S-O-R framework is used to unveil if some established shelf management techniques from physical stores are applicable in an online context. We also explain some of the cognitive processes customers experience as they get exposed to online buying situations. A main study with two data sources is conducted: one quantitative website experiment with 168 participants who got exposed to five different organisation patterns within five different categories; and one more qualitative eye tracking experiment with 20 participants. The results indicate that a disorganised product page evokes not only different scan paths and cognitive patterns but also generates higher sales.

The aim of the study was to look at a particular work group of housing supporters, whomhave implemented two special working methods in their work. The methods have been implemented by two of the housing supporters that also have special education in the methodsSOC (Sense of Coherence) and solution focused brief therapy. The study is based on anethnographic observation and analysis of documents that are used in the implementation. To analyse the information that was given at the observation and in the document I´ve used a theory which illustrates the significance of developing professions so their knowledge become legitimate. I´ve also used a theory that illustrates the importance of implementation and how it´s done at its best.

Abstract The purpose of writing this thesis was to improve methods during requirements engineering phase. Usercentred system engineering has some problem areas, which are examined and verified to create a new guideline for developers. This guideline tends to make requirements engineering more effective and help developers create more concrete requirements. It is not uncommon that system development projects ends up with unsatisfied users or delay in deliveries. The reasons are different kinds of communication problems between users and developers during verification of requirements. There is a therapy model, called solution-focused therapy, used in family and individual therapy. The model focuses on solutions for the future instead of problems in the past.

Background: Animals are used in several health care areas and have been shown to promote many aspects of patients? health. Animals have potential for use in nurses? health promotion work. Nurses need a deeper understanding of the importance and health-promoting properties of animal-assisted therapy.Aim: The aim was to describe the importance of animal assisted therapy for patients? health.Methods: A literature review was conducted based on ten nursing science studies with both qualitative and quantitative approaches, which were selected after critical review.

This study was conducted in the Master program at The Music Pedagogical Center (MPC) at The Royal College of Music in Stockholm. Korta musikresor (Short Music Journeys) is the Swedish term used for a receptive method of music therapy adapted from the tradition of The Bonny Method of Guided Imagery and Music (BMGIM) and the theory and practice of Expressive Arts Therapy. The KMR method uses carefully selected short pieces of music, lasting between 2 and 5 minutes, to work on various psychological problems and life- crises. The music session is conducted in a slightly altered state of consciousness with a clear focus being established beforehand in a verbal dialogue with the psychotherapist. There is also the possibility of using artwork to process and explore the imagery after the music listening.

Background: Sleep-disturbances in persons with Alzheimer´s disease are prevalent. They are characterized as increased frequency and duration of awakenings, decreased slow-wave sleep, REM-sleep and increase of day-time napping. Depression and lower quality of life are associated with sleep-disturbances. Altogether a significant source of stress for caregivers and an important factor in institutionalization. Non-pharmacological treatment alternatives are rare.Method: Single-case A-B-C design, with non-concurrent multiple baselines, a pilotstudy.Aim: To explore if an educational program on sleep, sleep disturbances and behavioural change directed towards caregivers improved sleep, depression and quality of life in persons with Alzheimer´s disease living at home.

The aim of this study is to gain an increased knowledge and deeper understanding of compulsive gambling and its consequences in today?s society. Gambling addiction will be described and analysed by a selection of experts, therapists and people with an addictive gambling background. The basis of the empirical research has been undertaken by a qualitative design with interviews consisting of three fundamental themes. The research group of eight participants included four gamblers, two therapists and two specialists.

The purpose of this study was to explore how interpersonal relationship can be affected by groing up in a New Religoius Movement such as Jehovah?s Withnesses and how it has affected them both in their youth and today. The aim was also to explore if there were people in the respondents' environment that was significant to them during their childhood and which support our respondents have received if they were in need of it.We have not found any previous research on children's social relationships within the Jehovah's Witnesses. We therefore believe that this study is of high relevance to both society and also for all the professionals who come into contact with these children. To discover a child in need, knowledge about how their social environment looks like is crucial.

The aim of this Master's thesis is to examine difficulties concerning information acquisition, use, and management experienced by graduate students in physics during their first years of research work. Semi-structured, qualitative interviews were carried out with nine doctoral students. The theoretical background for analysis of the material is based on the work of Patrick Wilson, with particular focus on the concept of cognitive authority, and on that of Lars Seldén, who has proposed the idea of an information seeking career running parallel to the academic career of the researcher. Results from the interviews indicate that most of the students, in the early stages of their information seeking careers, rely to a great extent on supervisors and more experienced colleagues when identifying material needed for the research project. In a situation where information of potential relevance is abundant and where experience and focus are not yet fully developed, senior researchers function as cognitive authorities and as guides to other cognitive authorities.