The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

The aim of this study was to investigate the effects of an infusion of hypertonic saline solution (HSS) in combination with an oral waterload on cows suffering from rightsided displacement of the abomasum (RDA). Six dairy cows were treated with 7,2% HSS in a dosis of 5 ml per kg bodyweight. They also received an oral waterload of approximately 15 liters. To monitor changes in fluid-, acid-base- and electrolyte-balance, bloodsamples were collected during the next 24 hours. During this period, the cows also underwent surgery to empty and correct the position of the abomasum.

High-precision cutting in metal is very complicated, a lot of parameters affect the result: what geometries to be manufactured, surface roughness and tolerances to be achieved, batch size etcetera. When pull-broaching internal parts this might be even more important, than in any other cutting operation. Everything is machined in one single stroke and the general technology is built in to the tool, therefore big investments must be made to change the process. Milling for an example: one piece at a time is machined away, and a lot of parameters can be changed easily.It is very high pressure on the cutting-edge when the broach is cutting. To achieve the specified product and to avoid unnecessary toolwear it is essential to use the right cuttingfluid and the right cuttingspeed for an example.

Background: Heart failure is a complex condition which means that the hearts capacity topump blood is reduced. The aim of this study was to investigate how patients with heartfailure considered their knowledge and the information they received about their disease andself-care.Method: Patients suffering from heart failure, hospitalized at section 30 E (n=13), andpatients visiting the nurse led heart failure clinic (n=4) at Akademiska sjukhuset in Uppsala,were requested to participate in the study. The patients answered questions about knowledgeand information concerning self-care and their disease. Data were analyzed using SPSS 15.0.Results: The patients valued their knowledge about the disease as lower than their knowledgeabout self-care. The study showed that all the patients found the information they hadreceived about their self-care better than the information they had received about theirdisease.

Marks do not only work as an acknowledge of the effort that has been made under a long period of time, but also as a receipt for eligibility to further studies. Within the political debate, marks have often caused a big discussion among the political parties. That is due to the obvious difference between the political parties on the left, and their counterparts on the right. However, there is one thing that they all have in common. Everyone of them have plans of measure within their political agenda, regarding the use of marks.The aim of this study has been to try to understand the way pupils think about marks.

Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine.

Peritonitis is a potentially life-threatening disease in horses. With no published work from Sweden, the objective of the study was to describe the occurrence and demographics of horses with peritonitis and to evaluate the outcome of treatment in terms of short-term survival during a ten-year period in a large clinic in Sweden. Data were examined in a retrospective manner in 69 horses diagnosed with and treated for peritonitis at Universitetsdjursjukhuset (UDS) in Uppsala between 2002-2012. Demographic data examined included age, breed, gender, history, duration of illness before arriving at UDS, reason for seeking veterinary care, initial clinical findings (general state of health, heart rate, respiratory rate, mucous membrane appearance, rectal temperature, abdominal sounds, rectal examination, nasogastric tube results), abdominocentesis results (abdominal fluid analysis including visual inspection, leukocytes and protein, cytology, bacterial culture and sensitivity pattern), complete blood count (CBC), Serum amyloid-A (SAA) upon presentation and a follow up, plasma fibrinogen, plasma protein and albumin, treatments, length of hospitalisation and outcome. All medical records with the diagnosis of peritonitis were extracted from the medical records system Trofast. All records with any other diagnosis code referring to trauma such as rectal tear or ruptured uterus, recent abdominal surgery, external trauma or rupture in the gastrointestinal tract were excluded. To be included in the study, the peritoneal fluid should contain more than 20.000 cells/?L, have a peritoneal protein value of >30 g/L; or have a significantly changed peritoneal fluid sample (orange with increased turbidity or worse) in cases where no data on cells or protein were available. The horses were divided into two groups; one where the peritonitis was deemed to have an idiopathic aetiology (primary peritonitis) and the other group where the peritonitis had a possible aetiology such as intestinal parasites, impaction etc.

The purpose of this essay was to describe eight female universitystudents drinking patterns and their own experiences of alcohol. The main questions that guided us through our study were:*How the female students describe the meaning of alcohol*How they describe their experiences of not drinking alcohol*If they have experienced pressure to drink alkcohol and if they have pressed other people to drink alcohol*How the students describe the student life influence on the alcohol consumptionA qualitative method was used as the purpose of this essay was to investigate the female students own subjective experiences and not to investigate students alcohol habits in general..

Laminitis is a debilitating disease causing much suffering to horses and ponies all over the world. It is manifested in the hooves as a mechanical failure of the suspensory apparatus between the hoof capsule and the distal phalanx. Due to the weight of the horse the disease may result in a dislocation of third phalanx. Six healthy standardbred trotters were used in this study on whether standardbreds tolerate forced recumbency as well as Shetland ponies. Recumbency was induced pharmacologically but maintained only by lowering the ceiling height of the box to a height of approximately 125 ? 140 % of the horse´s thoracic height.

Trade was assigned as a Community responsibility at the founding of the European Community in 1957. It was then recognized that Europe would achieve a greater international influence if it were to negotiate trade deals with one voice. Since then, the basic rule has been that the European Commission negotiates agreements on behalf of the EC, and presents the agreements negotiated for approval in the European Council. Today, the 25-member European Union is the largest trading power in the world, and most member states have decades of experience of coordinating their national trade interests into a common position. However, partly as a result of the complex nature of the trade decision-making processes it is sometimes problematic to identify who or what really influences EU trade policy.

Abstract Title: A conjoint analysis of packaging attributes. How can the attributes of a product?s packaging best look like to attract the consumer? Authors: Linda Granberg & Helena Niklasson Supervisor: Jan Svanberg Department: School of Management, Blekinge Institute of Technology Course: Bachelor?s thesis in Business Administration, 15 credits Purpose: The purpose is to find out how visual and technical attributes on product packaging affects the consumers tendency to want to buy the product. Method: Conjoint analysis and a quantitative survey. Results: When developing a package for a food product we?ve reached the conclusion that it is most important to have clear product information on it. The consumer wants it to be simple and easy to use, the people under time pressure seeks product with new technology which can save them time..

In this project the focus has been on the system for recombination at Oskarshamn 2. The recombining systems have on several occasions switched paths due to low pressure before the last ejector that?s feeding the recombiner. Of the mentioned reason, events and several measuring points have been analyzed in this report since the installation of the recombining systems.In order to prove theories and demonstrate what may be the cause of this problem, we used heat balance calculations by the program Probera. Using the program, we can demonstrate that the catalytic process does not reach the desired temperature with the amount of combustible substance.

Since drinking habits are created in social environments the workplace has an important role in alcohol prevention. This study has investigated employees (N=216) in a Swedish municipality work organisation with the purpose to describe the alcohol culture in workplace and how it influences on the workers attitudes towards alcohol. The theoretical baseline for the study has been Ames and Janes framework that explains the relation between workplace culture and drinking habits among workers. The study showed significant relations between restrictive attitude towards alcohol and not drinking with co-workers (POR: 2.5) and feeling low pressure in the work situation (POR: 2.2). Therefore it should be of interest for employers to adopt a cultural perspective and a primary prevention strategy in their alcohol policies that focuses on social relations and the workplace conditions.

Totally 84 differentially expressed mRNA clones from infective L3 larvae of the parasite Haemonchus contortus, a blood sucking nematode, were analyzed with single strand hybridization assay (SSH). Altogether 79 clones were sequenced, edited, and compared with proteins found via BLAST in GeneBank. The aim was to investigate gene expression and potential protein expression following storage at 5 °C for 32 weeks. mRNA was extracted from fresh and stored L3. The SSH derived products were cloned into E.

Apart from recognizing and neutralizing threats, antibodies also have the ability to regulate immune responses against their specific antigen. IgG administered together with erythrocytes is well known to induce >99% suppression. This has been used to prevent haemolytic disease in new-borns since the 1960's, but the mechanism behind it is still not completely understood. The effect on the primary response is well established, but its influence on induction of immunological memory is still heavily debated. In this study adoptive cell transfer of splenocytes to irradiated mice was used to survey the effect IgG has on induction of immunological memory without disturbance from the primary IgG response.