Amyotrophic lateral sclerosis is one of the major neurodegenerative diseases, causing an ascending paralysis that usually kills the patient within a few years from disease onset. The motor neurons show aggregates of proteins which in approximately 20 % of cases of the familial form contain mutated SOD1 protein. Trehalose is a disaccharide which has been shown to reduce protein aggregation and increase viability in cell models and alleviate symptoms in animal models of several neurodegenerative diseases associated with protein aggregation. When given orally to the SOD1G93A mouse model of ALS, trehalose failed to slow down the disease progression, which has led to questions about the uptake and distribution of the molecule in this mouse strain. The aim of this study was to investigate whether significant levels of trehalose reach the central nervous system of the SOD1G93A mouse after oral administration. This was performed by a trehalose assay of the brain of trehalose treated animals. A glucose assay was optimised for use in small samples of brain lysate after the digestion of trehalose into glucose by trehalase, and the difference in glucose concentration before and after digestion represented the trehalose level.

Incompatibility between donors and recipients can cause fatal hemolytic transfusion reactions (HTR), kidney damage and death and immunization of the recipient. Antigens on the surface of erythrocytes in the ABO blood group system and regular natural antibodies against these in the patient?s plasma must be considered. An individual always has a corresponding antibody of IgM in their plasma. Irregular antibodies acquired by transfusion or pregnancy and active in body temperature also cause the HTR incompatibility.

Flowcytometric technology has been widely used for measurement of the absolute numbers of T-lymphocytes subsets in Human Immunodeficiency virus (HIV), defining the disease state, monitoring antiviral treatment, and identifying any risk for opportunistic infections. A manual preparing of the samples is required. More recently an automated and enclosed blood cell counting, Celldyn Sapphire has been introduced. In this study the performance of the Flow cytometer EPICS XL as a reference method for analysis of CD3+, CD4+ and CD8 T-lymphocytes was evaluated with blood from 40 individual?s samples.

The purpose of this trial was to evaluate the possibility to use sampling of faeces, urine and plasma, and measurements of blood pressure as methods of discovering hypomagnesemia in horses, as well as the application handiness of these methods. The most commonly used method of checking the magnesium status of a horse is to analyze samples of serum or plasma. The trial was of a switch-back design, in which the horses were used as their own controls. The animal material was five Swedish warm blooded trotters between six to ten years old, and weighing between 485-620 kg. The trial consisted of three periods that were two months each.

Sahlgrenska Academyat University of GothenburgDepartment of Internal Medicine and Clinical NutritionAbstractTitle: Coconut fat?s effect on blood lipids - A systematic overviewAuthor: Linnéa Hult and Sandra WestlingSupervisor: Fredrik BertzExaminer: Ingrid LarssonProgramme: Dietician study programme, 180/240 ECTSType of paper: Examination paper, 15 hpDate: 2013-04-11Background: Coconut fat has recently been praised in the media. Amongst other things,coconut oil has been said to lower cholesterol (1, 2). This despite the fact that coconut oilcontains of 92 % saturated fat, which strong scientific evidence support raises the blood lipidsand thus increases the risk of cardiovascular disease (3).Objective: To examine the scientific evidence for how blood lipids in adults are affected byan intake of coconut fat compared to an intake of other kind of fats.Search strategy: PubMed and Scopus have been used in the literary search. Keywords usedwere "coconut fat", "coconut oil", "plasma lipids" and cholesterol [MeSH].Selection criteria: Human studies of RCT or cohort type, published since 1983.

BakgrundEn mer stillasittande livsstil med ett högre kaloriintag ökar riskerna för övervikt och andra metabola sjukdomar. För att förebygga och behandla dessa sjukdomar behöver vi bland annat förstå hur hungerkänslor regleras hos människan. Hur stor inverkan blodglukosnivåer har på hungerregleringen är dock omdiskuterat.SyfteSyftet med denna studie var att undersöka hur hungerkänslor och blodglukosnivåer förändras efter en måltid, jämfört med fasta. Kan den upplevda hungern påverkas genom periodisk fas-ta? Hur påverkar en invand måltidsrytm hungern? Hur påverkas hunger och blodglukos i för-väntan på en måltid?MetodTolv friska testpersoner (7 män, 5 kvinnor) där sju stycken var vana vid periodisk fasta och fem inte var det, randomiserades in i två grupper där ena gruppen (Pi) fick äta en 600 kcal pizza medan den andra gruppen fick fasta (F).

Today dairy farmers in Sweden are obligated to have lights turned on during night time in addition to the mandatory daylight inlet. This presents a cost for the production but many argue that it can be economically beneficial, especially in loose housing of dairy cows. In loose housing and even more so in herds with automatic milking, well functioning cow traffic during all hours of the day is crucial to optimise production. In order to keep the cow traffic flowing, lights may be an important management tool. It is also perceived among farmers with tied up stall that night light decreases the incidents of cows accidental stepping on their own or neighbours udders.

The aim of this study has been to examine how people with the chronic disease diabetes are seek-ing information about their disease. The study also look at the sources, which are mainly used, and the information behavior of the participants in this study. Especially in diabetes health care the idea of empowerment is central, which means that the patients need a lot of information to get know-ledge and become empowered. The study also examine the public library as a resource of health information, and for that purpose an examination of the OPAC catalog in two public libraries has been done, using the word ?diabetes? to illustrate the literature and other medias related to di-abetes.

Diabetes mellitus is characterised by persistent hyperglycemia in all species. Fructosamine is a glycated protein reflecting blood glucose concentration during the preceding weeks. It has become a valuable analysis to differentiate persistent hyperglycemia from transient stress induced hyperglycemia in cats. It is also used to evaluate current therapy in diabetic patients. Although fructosamine is a well accepted analysis, it is sometimes regarded with scepticism.

Background: Peripheral arterial disease (PAD), as a consequence of atherosclerosis in the arteria of the limbs, affects one of ten Swedes older than 65. PAD can develop slowly and silently, and involves an increased risk for cardiac infarction and stroke. Measurement of the patient´s ankle blood pressure and calculation of the ankle-brachial index is an evidence-based method to discover PAD. Objective: To investigate how district nurses and general practitioners perceive this method of examination and experience possible obstacles to why it is not more commonly applied at primary health care centres. Method: Data collected by semi-structured interviews have been processed by qualitative content analysis with a manifest onset.

Diabetes mellitus is a rising epidemic throughout the world and there is currently great interest in quantifying the beta-cell mass (BCM) in vivo non-invasively. In the present experiment, the feasibility of in vivo imaging of the glucagon-like peptide-1 receptor (GLP-1R) in beta-cells was examined, using the positron emission tomography (PET) tracer [68Ga]Ga-DO3A-VS-Cys40-exendin-4 as a marker, in native pancreatic beta-cells of a porcine diabetic animal model and healthy controls. Eight Swedish high-health domestic pigs were randomly assigned to be either controls or made diabetic using streptozotocin (STZ). The experiment proceeded during eight weeks, starting with an acclimatisation period. Once the pigs had been socialised they underwent surgery for the insertion of a jugular vein catheter, allowing induction of diabetes with STZ, intravenous (i.v.) injections and stress-free blood sampling. Development of diabetes was confirmed by clinical examinations, blood glucose values and insulin-staining of pancreatic sections post mortem. The diabetic pigs were insulin treated and responded well.

A number of studies in human medicine has documented positive effects of whole body vibration treatment in humans. The Vitafloor system, witch is based on treatment of horses with vibration, has the purpose to be used in training of healthy horses and as rehabilitation for horses with injuries in their musculosceletal system. The purpose of this study was to analyse what happens with a clinically healthy horse during treatment with Vitafloor. The methods used is mainly thermography before and after treatment, but also blood samples, measurement of rectal temperature, heart rate, clinical examination and observation of psychic reactions has been done. Statistic significant changes in skin temperature in hoofs and fetlocks of front legs has been observed after treatment with Vitafloor. Analyzing of the blood samples showed a tendency for decrease in Kalium, and two of the horses had changes in CK. No negative effect has been observed with the treatment of horses with Vitafloor, and the horses seems to like the treatment. .

The purpose of the study was to investigate whether or not Icelandic horses are less severely affected in clinical symptoms of cough or decreased performance than other breeds of horses. A breed variation in chronic respiratory disease severity was suspected based on clinical experience at the Institution for surgery & medicine, large animal, SLU Uppsala. A retrospective journal study of 17 Icelandic horses and 17 Swedish Standardbred horses with chronic problems with cough and/or decreased performance compared several measurements of disease severity. The clinical signs, physical examination and laboratory testing results compared were respiratory rate, abdominal breathing, lung auscultation, endoscopy results, broncho-alveolar lavage (BAL) results and the blood gases pO2 and pCO2. All parameters were available for all 34 individuals except for the blood gases, which were analysed, in only four Icelandic horses and four Swedish Standardbred horses. The only parameters that appeared different between the two groups were respiratory rate and pO2. The four Icelandic horses, which had blood gases analysed, had more severe hypoxemia.

The effect of total dietary fat on the blood lipid profile: a reviewFat consumption has increased in Sweden in the 21th century, probably due to the advancement of the diet low car high fat (LCHF). Also, cardiovascular disease is the leading cause of premature death in Sweden. Various blood lipids are used to evaluate the cardiovascular disease risk; total cholesterol, LDL, HDL and triglycerides among others. High levels of total cholesterol, LDL, triglycerides and low levels of HDL are believed to increase the risk. The purpose of this review was to examine the effects of high-fat-diet on blood lipids, cholesterol, LDL, HDL the ratio between LDL/HDL and triglycerides through a systematic review.

I denna studie har jag undersökt hur historiebruket i form av reproduktion av kulturarv ser ut i populärkulturen, närmare bestämt HBOs populära tv-serie True Blood och kopplar samman detta med hur vi ser på det föränderliga förflutna och därmed utvecklar vårt historiemedvetande. Uppsatsen står på tre ben och vilar på perspektiv där det första behandlar att man genom att se på reproduktionen av kulturarv kan finna samtidens idéer kring vad som är viktigt (meningsbärande), hur populärkulturen imiterar och återanvänder myter och därför kan anses vara en historisk källa värd att fokusera på samt historia som identitetskapande. Det teoretiska ramverket består av Judith Butlers teorier kring den heterosexuella matrisen och performativitet samt Gayle S Rubins teorier om sexualitet som någonting historiskt konstruerat. Uppsatsen ämnar öka kunskapen om betydelsen av hur historia som kulturarv i form av historiebruk förmedlas via populärkultur, närmare bestämt i tv-serien True Blood och hur detta hör ihop med hur vi uppfattar det föränderliga förflutna. Undersökningen tar fasta på hur stor del av rollfigurernas beteende i tv-serien True Blood som härstammar från deras historiska bakgrund och hur detta spelar in i utformandet av deras personligheter som tolkas ur ett genusperspektiv.