Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

Barley is one of the most important cereals cultivated in the Nordic countries. Climate change brings warmer and moisture climate which favors fungal diseases. In the cropland barley can be seriously infested with hard fungus attack. Since it is important that the yield bears a high quality it is of great importance to find varieties more resistant to attacks.Pathogenesis-related (PR) proteins are stress proteins induced in the plant in response to infection and abiotic stress (van Loon, 1997). PRs are shown to have antimicrobial activity differing between bacteria, fungi and oomycetes (Tandrup Poulsen, 2001).

Septic tenosynovitis involving the digital sheath is a relatively common problem in the horse and the prognosis has been considered as poor. However, there are only few studies confirming this. The purpose of this study is to increase the knowledge about the prognosis for septic tenosynovitis in the digital sheath. The literature indicates that half or a bit more than half of the treated horses will return to previous use. The recommended treatment is debridement of the wound, lavage and sometimes application of drains and broadspectrum antibiotics. The prognosis is considered as worse when the infection is established.

The aim with the literature study is to inform about the risks that exists in large dairy farms(with focus on mastitis and hoof health) and to inform about the milk industry in Sweden.Personal comments from two study visits (Nötcenter Viken and Vadsbo Mjölk AB) arelinked together with the literature and forms into a discussion regarding sustainableagriculture versus the milk cow's welfare.The profitability for Sweden's milk farmers have decreased because of the decreased milkprice and current inflation. This leads to an increase in herdsize in order to make a profit.The milk cow herds in Sweden becomes fewer year by year and the herdsize increases. Theincreased herdsize can cause a decrease in the time the farmer spends per animal. This canaffect early disease detection and prolong the animals suffering.Swedish Board of Agriculture published (2008) statistics showing that today's milk cow ismedicated primarily because of two reasons; mastitis or hoof problems. Mastitis is thereason to two thirds of all treatments that are carried out.

This study was the first part of a larger project investigating a new surveillance method for Echinococcus multilocularis. In this study we examined livers from wild boar (Sus scrofa), looking for lesions caused by E. multilocularis. E. multilocularis is a small tapeworm which has an indirect life cycle.

Barley is one of the most important cereals cultivated in the Nordic countries. Climate change brings warmer and moisture climate which favors fungal diseases. In the cropland barley can be seriously infested with hard fungus attack. Since it is important that the yield bears a high quality it is of great importance to find varieties more resistant to attacks.Pathogenesis-related (PR) proteins are stress proteins induced in the plant in response to infection and abiotic stress (van Loon, 1997). PRs are shown to have antimicrobial activity differing between bacteria, fungi and oomycetes (Tandrup Poulsen, 2001).

It is possible to affect the feed costs in different ways. By steeping or fermentation the digestibility of the feed is improved for the animals with a natural process in the wet feeding system Steeping means that the feed is soaked some hours before feeding. This process activatesenzymes and improves the digestibility of for example phosphorus in the feed and make it more accessible to the animal. Wet feeding can be fermented or not. Fermentationmeans that the feed has been soaked for a certain length of time so that a biological process is started.

Almost all people in Sweden have to visit a grocery store at some point, which are facing plenty of different kind of hygiene risks. They will face both employees and other customer - all of them are possible to give the innocent customer some kind of infection. Does the shopkeeper know this Maybe, maybe not. Nevertheless, there's a lot of work to do for a shopkeeper who wants to understand his customers. The shopkeeper needs to understand how to decrease the customers' risk perception, as well as how risk and media fits together.

Twenty-four calves, which had not grazed previously, were divided into four groups. They were subsequently inoculated orally with either E. alabamensis oocysts, O.ostertagi/C. oncophora larvae, or both. One group remained uninfected and was used as a control.

This report describes honey, its origin, properties and effects on human health. Honey has been used for a long time in most civilizations and apiculture is an important activity sector in many parts of the world. The trend is an increase in honey production but a decrease in the number of beekeepers in Sweden. Honeybees live in a highly developed society with a strong hierarchy and a well organized distribution of the tasks. They visit a broad spectrum of plants and collect nectar to store it as honey.

Over 40 miljon people in the world suffer from hiv/aids today. Africa is the continent that is worst affected by hiv/aids but how is the situation in Sweden? Why is it so quiet concerning hiv/aids in Sweden? Our aim with this work was to find out about the hiv/aids situation in Sweden and particulary in Malmö. The result of our research was that hiv/aids have increased since the year 2000 and most of the people had caught the infection in other countries than Sweden. This raised our curiosity concerning how immigrants/refugees are being treated when they arrive in Malmö and what are being done to stop hiv/aids from spreading further.We became very disappointed when we realised how little that is actually being done and how poorly the collaboration between the institutions are working.

Alla patienter som genomgår någon typ av operation där man penetrerar huden har en ökad risk att drabbas av en postoperativ sårinfektion. Syftet var att utforska de preoperativa åtgärderna huddesinfektion och hårborttagning relaterat till uppkomsten av postoperativa sårinfektioner. Metoden var en systematisk litteratur studie där nio artiklar användes som underlag. Resultatet visade att klorhexidin reducerar bakterierna på huden, men ingen signifikant minskning av antalet postoperativa sårinfektioner kunde påvisas. Av hårborttagningsmetoderna rakhyvel, rakapparat och hårborttagningskräm ger rakhyvel upphov till fler små sår..

Diseases associated with monocytosis in Swedish dogs - a retrospective study. The purpuse of this retrospective study is to determine which diseases are associated with pronounced monocytosis in Swedish dogs. Records of 153 dogs with monocytosis were evaluated. The most common cause was infectious diseases (49%) of which pyometra was seen in 23% of the cases, cystitis and fever of unknown origin each in 13% and gastroenteritis in 12%. Cortison treatment was the second most common cause with 29%. A sixth of the dogs had non inflammatory diseases such as neoplasia, renal- and heartfailure.

Salmonella in cats has been documented for many years. Infection with S. Typhimurium,which is often referred to as Songbird fever, is believed to be caused by birds whoinadvertently infect cats. The disease is often seen during the springtime when songbirdsreturn from their winter habitats, while other birds who have stayed during the winter are intheir weakest condition of health. In the spring of 1999 an epidemic of S.

Good feeding practices are a basic aspect of good calf health. During the calf's first few months the calf is fed with purchased milk replacer or raw milk straight from the cow. On all farms, it will be a surplus of milk that may not be delivered to the dairy for various reasons. Milk that may not be supplied to the dairy includes colostrum and milk with unnormal properties, including milk from cows suffering from mastitis. This study investigated the possibility of improving the milk by pasteurization in order to secure its use as feed for calves and thereby obtain an improved calf health.