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… of the work described in this bachelor thesis is to implement and opti-mize a real-time RT-PCR method for the detection of all genogroups of human sapovirus in foods. The optimization involves modifications of an already existing assay. Three synthetic DNA templates …
… previous PCR assay for detection of Influenza viruses as reference method. Real-time RT-PCR was used to compare Xpert Flu/RSV XC to the reference method. A dilution series was performed to estimate the methods? PCR efficiencies and precision was calculated from quadruplicates of …
… Campylobacter species, commonly C. jejuni and C. coli. In this study a triplex real-time PCR has been developed in order to be a last step in a qualitative method for identification of thermotolerant Campylobacter, originating from food and water suspected to have caused human …
… stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation. Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardiac infarction were analyzed by using …
… ELISA for walnut quantification was validated and compared with a qualitative real-time PCR. The rabbit antiserum proved to be less specific but after absorption with cross-reacting nuts and seeds it showed high specificity. The ELISA kit reacted, except for walnut, with pecan …
The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby …
… Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with …
… into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe …
an in vitro study of ovulation, implantation and receptivity in a mouse model
… ”, interleukin 1 ”, tumour necrosis factor ? and androgen receptor were used. Real Time PCR was run with the samples and gene expression was measured. The results of this study showed that the inhibition of IGF-1 receptor interacted with IGF-1 which lead to altered levels of …
… of the disease and the development of an effective vaccine. With quantitative real-time PCR and antigen ELISA has the levels of the cytokines IFN-? and IL-10 been studied in blood from SPF cats immunised with an ISCOM vaccine against FIPV and then challenged. The levels of IFN-? …
a model of disease transmission dynamics at the wildlife-livestock interface in Uganda
… Engorged ticks were collected from 6 warthog dens. These were analysed using real-time PCR for the presence of ASFV. All blood samples extracted from ticks were PCR negative for the virus. This study found one domestic pig that was PCR positive for ASFV, but negative for ASFV …
evaluation of kit for low-cost and fast preparing of DNA-samples before genotype analysis
… KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method. The purpose of the comparison was to find a method that makes DNA …
… Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR …
… in themenstrual cycle. The levels of specific mRNAs were measured with real-time PCR. Womenwho had become pregnant had a significantly higher level of steroid hormone receptors. Thus,these proteins seem to be important for a pregnancy and may be suitable as receptivitymarkers.
… of protection during experimental BRSV infection.Two one-step quantitative real-time PCR systems were developed and optimized in this work. One for detection of TLR4 mRNA, and the other for detection of the housekeeping gene product 28S rRNA. The assays showed good efficiency as …
