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Porcine circovirus type 2 (PCV2) is a common virus present in most parts of the world. PCV2 has been pointed out as the major causative agent to post weaning multisystemic wasting syndrome (PMWS) that affects pigs after weaning. The clinical symptoms of PMWS are impaired growth …
… Prcd-PRA and PLL. The analysis of eye diseases was performed with either real time PCR (TaqMan) or capillary electrophoresis (ABIPrism 3100). To gain further understanding about the ge-netic complexity and degree of genetic variation in the Scandinavian wolf population, the …
Invegon Equip AB
Real-time systems are required to answer to external stimuli within a specified time-period. For this to be possible, the systems behaviour must be predictable. The use of active databases in real-time systems introduces unpredictability in the system, e.g. due to their use of …
Conjunctival swabs from 12 cats in a Swedish animal shelter were analyzed by real-time PCR for FHV-1, Mycoplasma felis and chlamydiae. The purpose of this study was to see whether and to what extent these agents can be detected and if they were detected from cats housed in …
… was carried out using preordered primers and probes in the ABI 7900HT fast real-time PCR system. When evaluating the results two different disease haplotypes were detected, one longer and one shorter. These recombinant chromosomes helped to decrease the interval by 37 % …
… of controls, from three herds in Uppland, Sweden. The samples were analysed by real-time PCR (23S rRNA gene of Chlamydiaceae). When employing a threshold value of Ct 36, a significant difference in the occurrence of Chlamydiaceae between the entire case group (83% positive …
en studie av Rektorsgårdens trädgård i Uppsala
… of protection during experimental BRSV infection.Two one-step quantitative real-time PCR systems were developed and optimized in this work. One for detection of TLR4 mRNA, and the other for detection of the housekeeping gene product 28S rRNA. The assays showed good efficiency as …
… these bacteria with rapid and sensitive methods. In this study, Taq Man RT-qPCR was compared with SYBR Green RT-qPCR (LightCycler480, Roche) to explore which method had the best sensitivity with the least working hours. In addition, Bullet for automated DNA extraction and CAS …
… episode were selected. This resulted in seven horses for which results of real time-PCR mRNA to IL-4, IL-5 and IL-6 from their BAL fluid were compared between sampling episodes of low versus high mast cells. While there was no relationship between an increased level of mast …
… vaginal or preputial swabs respectively, were taken and analysed with rtPCR (real-time polymerase chain reaction) for the presence of chlamydia DNA. Furthermore, to investigate possible epidemiological factors connected with asymptomatic cats infected with chlamydia and to see …
