This project is a literature study with the hypothesis that Arctic climate has a positive effect on the accumulation of Metabolites in berries. Plant Metabolites are often divided into primary and secondary Metabolites. Primary Metabolites such as proteins and nucleic acids are essential for cell function, while secondary Metabolites are often non-essential, but have important functions regarding reproduction and survival. The berries mentioned in this project are from the families Ericaceae, Rosaceae, Elaeagnaceae och Grossulariaceae.The conclusion drawn from this literature study is that it is not obvious what favours accumulation of Metabolites in berries. Other factors aside from climate affect the production of secondary Metabolites, such as time of harvest, cultivars and ripeness.

During the last decades the fertility of dairy cows has declined in the same rate as the milk yield has increased. This is a result of the negative genetic correlation between milk yield and fertility, failure to show estrous signs and metabolic problems in the cow. The purpose of this review was to investigate the function of Metabolites and hormones as markers for disturbances in fertility, health and production in the dairy cow. There are several Metabolites that are suggested to indicate the cow?s energy balance.

The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their Metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2).The Chirobiotic V column showed high selectivity for citalopram and its Metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its Metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer.The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3.

Botanically diverse pastures are commonly used in New Zealand to reduce the ruminant environmental impact by reducing the methane production from the rumen digestion. In order to evaluate the effects diverse pasture species have on the milk fatty acid profile seventy-two lactating Friesian-Jersey crossbred dairy cows were used in a randomised block design with two replicates of six treatments. Six different pasture mixtures were fed at a daily allowance of approximately 15 kg DM/cow/day. The mixtures were categorised as either a simple or a diverse pasture. The simple mixtures all contained white clover with the addition of either a standard diploid perennial ryegrass (RG), a diploid high sugar ryegrass (HS) or tall fescue (TF).

The dairy cow?s ability to resume estrus after calving and to conceive is affected by their metabolic status after calving. For high yielding cows there is a high risk that the feed intake will not be sufficient to cope with the increased energy demands after calving, and the cow will end up in a state of negative energy balance. Energy balance can be measured in various ways, for example by body condition score or by plasma concentration of several metabolic hormones and Metabolites. After calving, endocrine pathways stimulate tissue mobilization; insulin signaling is blocked, the concentration of insulin-like growth factor-1(IGF-1) and leptin decreases, and the concentration of growth hormone (GH) increases.

SammanfattningMykotoxiner är sekundära metaboliter som kan bildas av vissa mögelsvampar. Idisslare har generellt ett högre skydd mot mykotoxiner än vad enkelmagade djur har då våmmens mikroorganismer kan fungera som ett extra skydd mot mykotoxikos. Trichotecenerna deoxynivalenol och T-2 toxin bryts ner till de-epoxymetaboliter i våmmen genom att dess toxiska epoxyring spjälkas bort. Deoxynivalenol metaboliseras helt eller delvis av våmmens mikroorganismer, medan T-2 toxinets nedbrytningskapacitet inte är lika hög. Aflatoxin B1 har en låg nedbrytbarhet, och inga metaboliter har kunnat detekteras i våmmen.

Acrylamide (AM) is a commonly used monomer in the manufacture of polyacrylamide. In addition, AM has been found in heated foods that are rich in starch. The main dietary sources in Sweden tend to be crisps, French fries, fried potatoes, crisp bread, cookies and coffee. Un-fortunately, dietary intake of AM has in several studies been shown to cause cancer in ro-dents. Consequently, the compound is currently regarded as a potential carcinogen in hu-mans.

The idea of using algae in different areas and exploring its possibilities is nothing new. Already in the early 1950s microalgae was explored as a possible food resource because of its high protein content. Lately there has been an increased interest in its potential for industrial use. Wastewater treatment is a possible use of algae since the algae grow well on the nutrients present in the water. This offers the possibility to grow algae for nutrient reduction and use the biomass for energy or animal feed since many alga species has a good nutritional value. Approximately 800-1000 m3 water is applied per 1000 m2 of greenhouse area during one season in a tomato production.

Microwave is a time- efficient device for heating and reheating of foods. Despite the fact that its effects on foods have been evaluated during a long period of time, without any more negative effects shown compared to conventional cooking, one third of the Swedish population believes (in 2010) it accounts for a higher loss of nutrition. This literature review aim to compile studies regarding how food is affected by cooking in microwave, with respect to cooking losses, nutrition, secondary Metabolites as well as sensory and textural aspects and compare it with conventional cooking. The compilation shows that different combination of effect (W), time and amount of water during microwave cooking have different effects on foods. The contents of proteins, vitamin B and carbohydrates are generally higher after microwave cooking, while anti-nutritional substances are affected much less during conventional cooking. This also depends on if it is of animal or non-animal origin. However, for the majority of nutrition values the differences are quite small.

Microwave is a time- efficient device for heating and reheating of foods. Despite the fact that its effects on foods have been evaluated during a long period of time, without any more negative effects shown compared to conventional cooking, one third of the Swedish population believes (in 2010) it accounts for a higher loss of nutrition. This literature review aim to compile studies regarding how food is affected by cooking in microwave, with respect to cooking losses, nutrition, secondary Metabolites as well as sensory and textural aspects and compare it with conventional cooking. The compilation shows that different combination of effect (W), time and amount of water during microwave cooking have different effects on foods. The contents of proteins, vitamin B and carbohydrates are generally higher after microwave cooking, while anti-nutritional substances are affected much less during conventional cooking. This also depends on if it is of animal or non-animal origin. However, for the majority of nutrition values the differences are quite small.

Microwave is a time- efficient device for heating and reheating of foods. Despite the fact that its effects on foods have been evaluated during a long period of time, without any more negative effects shown compared to conventional cooking, one third of the Swedish population believes (in 2010) it accounts for a higher loss of nutrition. This literature review aim to compile studies regarding how food is affected by cooking in microwave, with respect to cooking losses, nutrition, secondary Metabolites as well as sensory and textural aspects and compare it with conventional cooking. The compilation shows that different combination of effect (W), time and amount of water during microwave cooking have different effects on foods. The contents of proteins, vitamin B and carbohydrates are generally higher after microwave cooking, while anti-nutritional substances are affected much less during conventional cooking. This also depends on if it is of animal or non-animal origin. However, for the majority of nutrition values the differences are quite small.

Soapnuts have been used as a cleaning detergent and medicine since ancient times in different parts of Asia. Today soapnuts are sold as an environmentally friendly alternative to common washing detergents. The aim of this literary study is to give an answer to what it is in the soapnuts that makes them work as a washing detergent. The active component belongs to a group of components called saponins which are secondary Metabolites with the purpose to protect the tree from pathogen attacks. Saponins are widely spread in the vegetable kingdom and some of the families and species are so rich in saponins that their names are influenced by this, for example Sapnindaceae and Sapindus which are mentioned in the beginning of this literary study.

Phytoestrogens are secondary Metabolites produced by plants. Among the plants that make phytoestrogens are soya, red clover and some types of grass. The name phytoestrogen is a collective term for compounds such as genistein, daidzein, biochanin A and formononetin. These compounds are similar to oestradiol-17? in their chemical structure.

Catecholamines are stress hormones that are produced and released by a rare tumor called pheochromocytoma. This tumor can cause hypertension which if undiagnosed and untreated leads to death. Since good therapy is available, it is important to find the tumor in time. The most common way to diagnose the tumor is measurement of the biochemical markers; catecholamines and their Metabolites, methylated catecholamines.After observation that almost all normetanephrine results for women were higher than the upper reference limit and therefore pathological, the accuracy of the present reference intervals was questioned. Therefore new reference intervals for both urinary catecholamines and methylated catecholamines were developed by analysis of 46 samples using HPLC.

5-hydroxytryptamin (5-HT) is biochemically derived from Tryptophan. Kynurenic acid and quinolinic acid are also known Metabolites of tryptophan. 5-HT is considered to play a role in depression and today selective serotonin reuptake inhibitors (SSRIs) are used for treatment of depression. 5-HT may also play a role in pain perception. The aim of this study was to investigate whether a supplement of tryptophan may affect conditions such as depression and/or pain.