Ten colic horses and seven healthy horses were included in a prospective study aiming to evaluate acid-base disturbances in colic horses. Traditional acid-base methods and measurements of Lactate were used to study prognosis and changes before, during and after surgery. Strong ion gap, anion gap and base excess were compared to evaluate their ability to predict Lactate. Of the ten colic horses that went through surgery, seven survived, two were euthanised and one died. Significant differences in plasma Lactate levels were seen between the group of survivals and non-survivals, p=0,000025. No differences were seen in pH and base excess between the two groups.

The purpose of the present study was to explore if blood Lactate levels are increased in bitches with pyometra, and if the Lactate measurement could be used as a prognostic indicator and to determine outcome. The present study included 16 bitches with pyometra that were admitted to the University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden. For comparison, a control group consisting of 14 healthy staffowned bitches was also evaluated. In all bitches, a physical examination was performed and blood samples for analysis of haematological and biochemical parameters were obtained. In all bitches with pyometra, surgical ovariohysterectomy was performed and a uterine sample for bacteriological culture and identification was collected.

The equine hoof is often manipulated in purpose to achieve a better stride or to reduce the weight on damaged tissue. The living tissue in the hoof can?t store energy as glycogen and is therefore depending on a continuous supply of glucose for its energy production. The purpose of the study was to determine if wedging (i.e. elevating) the heel could influence the hooves glucose and Lactate consumption/production. Five standardbred horses were trained to trot on a treadmill without getting physical or mentally tired.

Sport horses are widely used for performance in jumping, dressage, endurance etc. To enable comparisons of horses, training facilities, programs or physical status, an exercise test can be used. Exercise tests are also important to increase knowledge about exercise physiology of the horse considering different ways of training to compare training methods or individual qualities, development of an individual or other investigations of equine exercise physiology. Since several investigations are done on standard- and thoroughbred horses but less on sport horses, it is of great importance to get an overview of the investigations done. Most common is to investigate heart rate and Lactate concentration.

The main objective of this research was to study how increased arterial oxygenation by the use of Pulsed Inhaled Nitric-Oxide (PiNO) influenced the metabolic and cardiovascular parameters on horses undergoing acute abdominal surgery because of colic. The parameters blood Lactate, blood glucose, pH and heart rate were evaluated before and during anesthesia and closely after recovery. The study showed that blood Lactate concentrations decreased significantly during anaesthesia in horses treated with PiNO compared to non-treated horses. It was also seen that the Lactate concentration decreased most in horses with the highest levels before PiNO treatment. Since enhanced oxygen extraction ratio was evident in the PiNO group, improved oxygen delivery to the tissue may be a possible explanation for the improved situation.

Phosphorus (P) is an essential nutrient but a surplus could lead to eutrophication. Furthermore, the minerals used to produce the mineral phosphate is a non renewable resource. There are in other words several reasons to reduce the leakage of P and the agriculture is one important area to attend. The aim of this work has been to investigate if carbon-rich arable soils content contain more P than arable soils with low carbon content. Moreover it has been calculated if the carbon-rich soils could pose a risk of P leakage by comparing their delivery of P with the plant uptake. The work has consisted of compiling the results from former gathered and analysed soil samples of 130 Swedish arable soils. The fractions of P considered are water soluble P (Pw), ammonium Lactate-extractable P (P-AL), hydrochloric acid-soluble P (P-HCl), organic ammonium Lactate-extractable P (Porg-AL) and inorganic ammonium Lactate-extractable P (Porg-HCl).

Background: Practical blood flow restriction (pBFR) combined with resistance training has been proven to facilitate muscle strength despite training at low intensity. However, research investigating the effects of long-term pBFR-training and its periodization with strength training (ST) at a higher intensity is lacking, as well as the effects of occlusion training on blood Lactate levels (BLL) over time.Purpose: To compare the effects of 2 weeks of pBFR training followed by 2 weeks of ST (BFR-ST) with 2 weeks of ST followed by 2 weeks of pBFR training (ST-BFR) on anaerobic performace and BLL.Method: 5 adults (age 23 ± 2 years) with a minimum of 1 year of ST experience were recruited and randomized into BFR-ST (n=2) or ST-BFR (n=3). Anaerobic performance was measured using a 30-s Wingate-test and BLL samples were collected <1 and 5 minutes post-Wingate. Data and samples were collected before the intervention and after the 2 protocols.Results: No differences were seen between groups in anaerobic output or BLL after 4 weeks of training (p>0,1 for all values). A decrease in time to peak power (tPP) were seen in both groups with an advantage for BFR-ST (-37 ± 8 %) over ST-BFR (-27 ± 22 %).

Eutrophication of the Baltic Sea is a serious problem. A major contributing factor is diffuse losses of phosphorus (P) from agricultural land in surrounding countries. In order to estimate P losses, environmental monitoring of small agriculture-dominated catchments is being carried out in most of these countries. Evaluation of the risk of P leaching to waters is usually based on chemical tests originally developed to quantify the amount of soil P available for plant production. The tests are performed in different ways in the different countries and a number of different extraction agents are in use.

Introduction: This study aimed to investigate the validity and reliability of the 20m multistage shuttle run test (MST) for elite ice hockey players as a predictor of the maximal aerobic capacity and the how the result is affected by the players age, aerobic and anaerobic capacity, body composition and test surface. The purpose of this was to find out the potential of the 20-m shuttle run test as a measuringtool for the maximal aerobic capacity in frequent testing periods during the season. It is very interesting to be able to administer this easily because the competative phase of the season is very long compared to the pre-seasonal phase and earlier studies have shown that the aerobic capacity is being lowered during this competative phase of the season for elite icehockey players.Methods: 34 elite ice hockey players participated in this study (20.0±3.6 yrs, BMI 24.2±1.6 and VO2-max 58.0±4.8 ml O2?kg-1?min-1). During 3-5 days they underwent 2 MST tests (wood and rubber surface), treadmill running with VO2-max assessed with open-circuit technique, Wingate10% and FFM-assessment with underwater-technique.

Different field tests are currently used in a wide extent in different levels of soccer teams. To be able to assess the players? physiological status the tests must have high validity, reliability and external validity. Many different tests have been developt, but currently only five of these tests are most commonly used: Yo-Yo intermittent recovery test (YIRT), Yo-Yo intermittent endurance test (YIET), Multi-sprint-test, Beep-test and Cooper-test. Presently there is no definitive conclusion regarding which of these tests is most suitable for soccer players.

The bacterium Lactobacillus reuteri, which natural habitat is in the intestine of mammals and birds, uses glycerol as a substrate for production of the antimicrobial compound reuterin. Glycerol has been shown to decrease the number of Escherichia coli in human feces and it is believed to be due to in situ production of reuterin. E. coli belongs to the large family of Enterobacteriaceae, naturally occurring in the intestine, and pathogenic strains of E. coli have been shown to be one of several bacteria causing diarrhoea in calves.

The short term effect of phosphorus in sewage sludge was investigated to establish howmuch of this a farmer can grant him- or herself, if using sewage sludge as a phosphorusfertilizer. Possible differences in plant availability between sludge phosphorus precipitatedby iron, aluminum or calcium integrations were studied in a sandy soil at two pH levels.The sludges were analyzed using pot and incubation trials. As the intention was to investigategrowth limitation due to phosphorus deficiency, a soil with low phosphorus supplywas chosen as well as a low phosphorus application rate. Sludge corresponding to 12kg phosphorus per hectare was applied. Treatments fertilized by sludge were compared totreatments where 0, 6 and 12 kg phosphorus per hectare from mineral fertilizer was applied.Every treatment was replicated six times.

Denna studie gjordes på förfrågan av en simklubb i västra Sverige. Uppgiften var att mätafram laktatkurvan på ett antal simmare. Önskemålet var tio (10) simmare men vi fick ettbortfall på fyra (4) på grund av sjukdom, ovilja och tester för nära inpå mästerskap. Metodenvi valde var laktatmätning efter varje simmat lopp. Simmarnas uppgift var att simmaprogressivt 5*200m frisim.

The short term effect of phosphorus in sewage sludge was investigated to establish howmuch of this a farmer can grant him- or herself, if using sewage sludge as a phosphorusfertilizer. Possible differences in plant availability between sludge phosphorus precipitatedby iron, aluminum or calcium integrations were studied in a sandy soil at two pH levels.The sludges were analyzed using pot and incubation trials. As the intention was to investigategrowth limitation due to phosphorus deficiency, a soil with low phosphorus supplywas chosen as well as a low phosphorus application rate. Sludge corresponding to 12kg phosphorus per hectare was applied. Treatments fertilized by sludge were compared totreatments where 0, 6 and 12 kg phosphorus per hectare from mineral fertilizer was applied.Every treatment was replicated six times.

One of the most serious and economically important defects caused by clostridia in milk products is the late blowing of semi-hard cheeses.Clostridia occur naturally in soil and can contaminate milk through crops contaminated by dung and soil followed by a less successful silage process, that give them opportunity to grow unaerobically. When anaerobic conditions occur, such as storage of semi-hard cheese, they ferment lactic acid to butyric acid and the gases CO2 and H2.At the fusion of Arla and MD Foods, a series of changes were conducted on the MPN method for lactic acid fermentation for clostridia in milk. These changes resulted in an increased accuracy due to an increased number of test tubes and the change of media from MRCM to BBB, Bryant & Burkey Broth, that was thought to be more selective for Cl. tyrobutyricum, the organism mostly found in hard cheese. When the number of dairy farmers that were given quality reduction fines increased, the new method was suspected and a validation was conducted.The validation included inoculation of different clostridia and bacillus strains into BBB substrate and enzymatic testing of positive samples with Rapid ID 32A.