This project investigated how elevation and vegetation type influences variation in plant litter phenolic content and protein precipitation capacity among and within common plant species for two different vegetation types, heath and meadow, in a subarctic ecosystem in the Abisko region of northern Sweden. As nutrient availability generally decreases with increasing elevation as a result of decreasing temperature, I hypothesised that phenolic content would increase with elevation and be higher on the heath than the meadow. To test this, the total phenolic content and protein precipitation capacity was estimated in leaf litter from 13 species in both heath and meadow vegetation across an elevational gradient ranging from 500 to 1000 meters above sea level (m.a.s.l.) in the study region. The results showed that elevation and vegetation type both had a strong impact on both variables. Total phenolic concentrations decreased with elevation for the meadow, and were greater for the heath than the meadow.

The unique ability of wheat to produce leavened bread is mainly due to the gluten proteins present. As consumers are more and more attracted to bread with high fibre content the use of wheat gluten will also increase in order to obtain bread with good volume and appetizing characteristics. Air-classification is a technological method used to separate particles by size and shape by means of air-streams into two fractions, fine and coarse. When used on wheat flour it is known to alter the flour composition in the fractions obtained compared to the original flour. This method is not widely used in the milling industry but could possibly be of interest if protein rich fractions with favorable protein quality can be produced.

Hur varierar proteinsammansättningen i ölet genomölbryggninsprocessen och hur stor skillnad är det mellan kornmalterna. Syftet med mitt examensarbete var att se hur proteinsammansättningenförändrades under olika steg av en ölbryggningsprocess.Proteinsammansättningen delades in i 3 grupper: 1) albuminer ochglobuliner, 2) hordeiner och 3) gluteliner. Dessa protein jämfördesmellan 2 sorters malt för att se om någon skillnad kunde ses.Ölen bryggdes efter ett standardrecept för pilsneröl. Under ölbryggningsprocessen togs prover ut vid bestämda steg och tidpunkter.Proteinsammansättningen bestämdes genom analysmetoden SDS-PAGE.Resultat: visade att ingen synbar skillnad på proteinsammansättningen mellan malterna kunde ses.Proteinsammansättning i de olika stegen var däremot tydlig, albumineroch globuliner är de enda protein som finns kvar i det färdiga ölet, varavde flesta är z-protein. ..

Mast cells have for a long time been known to accumulate around tumors (Maltby et al., 2009). Studies show that they may be important and sometimes essential in tumor angiogenesis, tumor growth and metastasis of tumors (Soucek, et al., 2007; Xiang et al., 2010). In an expression array study (Wensman et al, submitted manuscript) performed on mouse mast cells exposed to tumor conditioned medium, the gene 4-1BB was among the most up-regulated genes compared to control medium. 4-1BB codes for a membrane receptor protein of the tumor necrosis factor superfamily and has been shown to be important in T cell regulation in tumor diseases. This study was performed to investigate if human mast cells up-regulate 4-1BB when they get exposed to tumor conditioned medium.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

The occurrence of small amounts of albumin in urine, microalbuminuria (MA), is used both in human- and veterinary medicine as an indicator for patients who have developing proteinuria. Proteinuria can be prerenal, renal or postrenal which is important to establish during an investigation. With conventional methods decreased renal function is not detected until 70 % of renal function is lost. The ability to detect and start a treatment earlier might reverse or slow the progression of disease. The purpose of this study was to investigate the reliability of Heska® E.R.D.-Health Screen?, a test for microalbuminuria in dogs, to detect albuminuria.

This study was a degree in Master of Science in agriculture with specialization in animal science for the Department of animal nutrition and management at SLU. The subject was created by AS-Faktor, a small company for science and development within the company Lantmännen AB. It was a test of the effect of SPC (Special Processed Cereals) on induction of the endogenous protein AF (an antisecretory factor) in plasma and intestinal lumen in broilers. Previous results have shown positive effects on different intestinal diseases in pigs, horses, dogs as well as in humans. Beside the induction we also studied the effects on results in a conventional slaughter chicken production, including the ability of SPC to compete with or be affected by the coccidiostat, Salinomax.When diarrhoea occurs, it's because there is an imbalance between the secretion and the absorption and it can be caused by for example toxins.

Totally 84 differentially expressed mRNA clones from infective L3 larvae of the parasite Haemonchus contortus, a blood sucking nematode, were analyzed with single strand hybridization assay (SSH). Altogether 79 clones were sequenced, edited, and compared with proteins found via BLAST in GeneBank. The aim was to investigate gene expression and potential protein expression following storage at 5 °C for 32 weeks. mRNA was extracted from fresh and stored L3. The SSH derived products were cloned into E.

Syfte: Syftet med denna systematiska litteratur?versikt ?r att unders?ka om supplementering av vassleprotein har n?gon p?verkan p? bent?thet (BMD) hos vuxna ?35 ?r. Metod: Litteraturs?kningen gjordes i tv? databaser, Pubmed och Scopus. S?kord f?r blocken var vassleprotein, BMD och randomiserade kontrollerade studier (RCT). Deltagarna i de inkluderade studierna var m?n och kvinnor ?35 ?r d?r interventionsgruppen fick supplementering av vassleprotein medan kontrollgruppen fick supplementering med likv?rdigt energiinneh?ll som ej inneh?ll vassleprotein.

This project investigated how elevation and vegetation type influences variation in plant litter phenolic content and protein precipitation capacity among and within common plant species for two different vegetation types, heath and meadow, in a subarctic ecosystem in the Abisko region of northern Sweden. As nutrient availability generally decreases with increasing elevation as a result of decreasing temperature, I hypothesised that phenolic content would increase with elevation and be higher on the heath than the meadow. To test this, the total phenolic content and protein precipitation capacity was estimated in leaf litter from 13 species in both heath and meadow vegetation across an elevational gradient ranging from 500 to 1000 meters above sea level (m.a.s.l.) in the study region. The results showed that elevation and vegetation type both had a strong impact on both variables. Total phenolic concentrations decreased with elevation for the meadow, and were greater for the heath than the meadow.

Scrapie is an infectious neurologic disease in sheep caused by prions, corresponding to transmissible spongiform encephalopathies (TSEs) in other species. The prion is presumably constituted of PrPSc, the misfolded form of the normal endogenous prion protein, PrPC, which is found in practically all cells in the body. PrPSc can induce a conformational change in PrPC and misfold it as an imprint of itself; this is how prions amplify and spread. The process of conformational change is poorly understood and there might exist intermediate forms between PrPC and PrPSc. Bioassay using mice has traditionally been the golden standard in prion studies but is limited by cost and time.

The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

The cattle production in the world has been questioned in Sweden due to its negative impacts on the environment. The use of imported soya bean meal as a protein feed for Swedish cattle results in the cutting of rainforest and an extensive use of pesticides in the countries were the soya beans are produced. As a consequence, the environmental impacts caused by cattle production increases. A production of protein feeds in Sweden would decrease the transportations and the cutting of rainforest. For organic beef producers the possibility to produce home-grown protein feeds is important since organic protein feeds can be difficult to obtain in another way.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

This project investigated how elevation and vegetation type influences variation in plant litter phenolic content and protein precipitation capacity among and within common plant species for two different vegetation types, heath and meadow, in a subarctic ecosystem in the Abisko region of northern Sweden. As nutrient availability generally decreases with increasing elevation as a result of decreasing temperature, I hypothesised that phenolic content would increase with elevation and be higher on the heath than the meadow. To test this, the total phenolic content and protein precipitation capacity was estimated in leaf litter from 13 species in both heath and meadow vegetation across an elevational gradient ranging from 500 to 1000 meters above sea level (m.a.s.l.) in the study region. The results showed that elevation and vegetation type both had a strong impact on both variables. Total phenolic concentrations decreased with elevation for the meadow, and were greater for the heath than the meadow.