Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral Vectors integrate into the chromatin of target cells and do not transfer any viral genes causing Vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralVectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

Increased trade and eased policy restrictions have brought markets closer together. Prices at different locations are much likely to affect each other to a certain extent. Since the adaption of Common Agricultural Policy in 1995 the Swedish wheat market has been exposed to the world market and Swedish farmers are facing new challenges. A broader knowledge about market integration and price transmission will facilitate Swedish farmers, banks and politicians in making rational decisions. Therefore the aim of this research is to explain how global wheat prices are transmitted on Swedish wheat prices.

Japanese encephalitis virus (JEV) is a significant Vector-borne zoonotic pathogen, causing devastating encephalitis in humans. Its geographical range includes a majority of Asian countries and has also been recognized in some western Pacific areas. The main Vectors of JEV are mosquitoes belonging to the genus Culex. Birds and pigs function as hosts and virus amplifiers, whereas humans are accidental hosts. Japanese encephalitis is commonly regarded as a rural disease.

Abstract CORDIC (Coordinate Rotation Digital Computer) is an iterative algorithm for the calculation of a two-dimensional Vector in circular, linear or hyperbolic coordinate systems. This paper presents a survey of known CORDIC algorithms and architectures for the rotation and Vectoring mode in the circular cordinate system. In addition an implementation of the differential CORDIC algorithm in VHDL has been done. The implementation is designed to keep the fast timing and throughput characteristic known for on-line redundant arithmetic..

Induction motors, often referred to as the workhorse of the industry, play an important role in production today. Therefore, it's important to diagnose any faulty induction motor prior to failure to avoid costly and time consuming production stops.This thesis compares methods for diagnosing induction motors in operation, MCSA, Park's Vector approach and the standard method used by Swedish industries today. By simulating broken rotor bars and short-circuit in one of the windings, each method is tested and the results are compared using matlab.The results need further verification due to uncertainties of the condition of the test motor, even though the experiment gives a hint of the different advantages of each method. A combination of the methods should be able to tell if the motor needs further examination..

This project is a learning CD-ROM production aimed at first-time parents. It is using both sound and images, moving as well as non-moving. The purpose of the production is to be an interesting alternative to traditional books. We have been looking at similar productions like drivers license educational CD-ROMs and encyclopedias for inspiration and ideas on how to proceed. In order to give the content some dynamics, we've also added the element of 3D-animated sequences.

This project is a learning CD-ROM production aimed at first-time parents. It is using both sound and images, moving as well as non-moving. The purpose of the production is to be an interesting alternative to traditional books. We have been looking at similar productions like drivers license educational CD-ROMs and encyclopedias for inspiration and ideas on how to proceed. In order to give the content some dynamics, we've also added the element of 3D-animated sequences. The application is supposed to be run as a simple executable file with flash embedded.

We derive the equation linking the Weyl tensor with its Lanczos potential, called the Weyl-Lanczos equation, in 1+3 covariant formalism for perfect fluid Bianchi type I spacetime and find an explicit expression for a Lanczos potential of the Weyl tensor in these spacetimes. To achieve this, we first need to derive the covariant decomposition of the Lanczos potential in this formalism. We also study an example by Novello and Velloso and derive their Lanczos potential in shear-free, irrotational perfect fluid spacetimes from a particular ansatz in 1+3 covariant formalism. The existence of the Lanczos potential is in some ways analogous to the Vector potential in electromagnetic theory. Therefore, we also derive the electromagnetic potential equation in 1+3 covariant formalism for a general spacetime.

Intervjuer med kunniga på AIS-området har utförts parallellt med en enkät riktad till aktiva befäl ute till sjöss. Detta i syfte att ta reda på dels hur detta hjälpmedel bör användas vad avser antikollision, dels hur det faktiskt används i praktiken.AIS, Automatic Identification System, är ett informationsdelningssystem som hjälper fartyg att navigera säkert genom att sända ut egen navigationsdata och samtidigt ta emot motsvarande information från andra fartyg. Systemet nyttjas även som informationssystem för landbaserade stationer såsom VTS-centraler och kustbevakning.Undersökningen har engagerat och visat att expertisen anser att AIS, i många avseenden, är ett bra och användbart hjälpmedel som bör komplettera övrig utrustning vid bedömning om kollisionsrisk. Vidare visar enkäten att ARPA-systemet, trots vissa inneboende brister, tillmäts större förtroende än AIS och AIS-vektorer används därför sparsamt. Enkäten visar även att en stor del av de aktiva inte är främmande för att göra avsteg från sjövägsreglerna emedan expertisen i huvudsak avråder från sådant..

The genus Borrelia is a widespread, pathogenic pest and the causative of among others borreliosis or Lyme disease. The Vector for the bacteria is the hard tick, Ixodes ricinus and the infection is transferred through a bite. Untreated, Borrelia may cause arthritis, heart damage or neuroborreliosis. Infection is made possible through different strategies for avoiding the body?s complement system.

Några av de ändringar som gjordes 2011 i läroplanen för gymnasieskolan var att vektorer lades in i det centrala innehållet för ämnesplanen Matematik 1c. Denna studie behandlar utformandet av uppgifter som testar elevers förståelse för vektorbegreppet där urvalet omfattar gymnasieelever som har läst eller läste kursen Matematik 1c inom perioden för uppsatsen. Metoden som användes var semistrukturerade intervjuer där uppgifterna agerade frågor och eleven fick svara på följdfrågor så att tankebanor, funderingar och hinder skulle kunna observeras.Svaren som eleverna har gett på de uppgifterna har skapat ett brett spektrum av olika svar, en svarsrymd. Denna svarsrymd har sedermera blivit svarsalternativen till det slutliga test som studien har arbetat fram. Testet har således beprövade uppgifter som är skapade med en rad olika områ-den som utgångspunkter.

This thesis compares four relevance feedback methods. The Rocchio and Ide dec-hi algorithms for the Vector space model and the binary independence model and Okapi BM25 within the probabilistic framework. This is done in a custom-made Information Retrieval system utilizing a collection containing 131 896 LA-Times articles which is part of the TREC ad-hoc collection. The methods are compared on two grounds, using only the relevance information from the 20 highest ranked documents from an initial search and also by using all available relevance information. Although a significant effect of choice of method could be found on the first ground, post-hoc analysis could not determine any statistically significant differences between the methods where Rocchio, Ide dec-hi and Okapi BM25 performed equivalent.

Modern agriculture equipment are more computer based today, and many equipments use a terminal in the tractor where the driver have the opportunity to make adjustments to the equipment. This is the reason why ISO developed a new standard called ISOBUS. It is a communication standard based on CAN specially adapted for griculture equipments. The purpose of the standard is that it should be ossible to equip a tractor with a standard terminal called Virtual Terminal that can be used to control the equipment. The use of the terminal should be independent of the manufacturer of the tractor as well as of the equipment.The purpose of this report is to ?nd a solution of how to use CC Systems on-board computer, CCP XS, as a Virtual Terminal.