Background: Mastitis in dairy cattle is a common ailment worldwide. A cause of mastitis can be bacteria such as Escherichia coli. Mastitis is not a deadly ailment and sometimes the dairy cows show no symptoms but if certain virulence genes are present in the bacteria that cause the mastitis, the bacteria can be transmitted to humans and cause severe diseases. The potential presence of enterohemorrhagic Escherichia coli (EHEC) in particular would be a major concern for human health.Aim: The aim for this study was to analyze the presence of virulence genes known to be present in E.coli strains isolated from dairy cows with mastitis in Sweden.Method: A Qiagen BIO ROBOT EZ1 was used to purify DNA from 90 bacterial cultures. A panel of virulence genes were amplified and biotinylated from the purified DNA by PCR and an E.coli based DNA microarray was used to detect presumed virulence genes in E.coli.Result: There were no samples that had all the genes traditionally used to classify E.coli as EHEC or potential EHEC.

Denna undersöknings syfte har varit att utreda när och hur subkulturen klubbkultur växte fram i Malmö, vilka som tog den hit, vad som då definierade kulturen och hur detta skiljer sig från idag.Ytterligare syfte har varit att undersöka hur klubbkulturen som sägs genomsyras av en universal jämlikhet, på olika sätt, såväl inkluderat som exkluderat människor från att delta i den. Genom kvalitativa intervjuer med sju aktörer aktiva inom klubbkulturen under det sena åttiotalet och tidiga nittiotalet, men också genom min egen bakgrund som besökare, arrangör och DJ, målar detta arbete med hjälp av muntlig historia, ett kulturanalytiskt perspektiv och med Sarah Thorntons teorier om klubbkultur och subkulturellt kapital upp historien av hur Malmös klubbkultur växt fram samt de viktiga faktorer som definierade den och på vilka sätt kulturen både exkluderade och inkluderade. Resultatet berättar en historia om en ny upprorisk motkultur som under det tidiga nittiotalet utvecklades från att ha varit en brett inkluderande, men samtidigt en underjordisk gör-det-själv-kultur, till att bland annat genom tyst exkludering i form av nischade smakgemenskaper och konsumtionssymboler, utvecklas till en uppdelad kultur som inte bara positionerade sig mot det så kallade mainstreamsamhället utan även mot andra klubbkulturer..

Today there are various methods to use when euthanizing horses. Earlier the use of free or captive bullet was the most common method, but in recent decades this has changed and chemical euthanasia is now considered the most common method. The chemical euthanasia has in Sweden been dominated by the use of barbiturates. Somulose® entered the market in the beginning of the 90?s as an alternative to previous chemical euthanasia.

This thesis has been performed at Clinical Chemistry at Sahlgrenska University Hospital in Gothenburg. The purpose of the project was to investigate new and alternative ways to determinate synthetic cannabinoids by gas chromatography-mass spectrometry. Currently, the possibilities to quantify synthetic cannabinoids are very limited. This can lead to an increased use of synthetic cannabinoids as the risk of detection is low, which may be known by drug users. The synthetic cannabinoids are sold mixed with different herbs and have varying names like Spice Gold, Spice Silver, K2, Smoke and Pot-pourri.The synthetic cannabinoids analyzed were JWH-018 and JWH-073, which are commonly found in seized Spice material.

I want to look at the possibilities to start selling hot water from a small district heating plant with bioenergy. My reason for doing this project is to look at a new business opportunity for our farm. Our company have less work during the winter time compared to the spring and this project will give us more work during the winter. I also want to take as much material as possible for heating from our farm in order to increase the value of the products from the farm. The project is in a village near us, where we should support a school, a churh, a home for the aged, a kindergarten and a parish hall with heat. In my work I have compared two different alternatives with each other. The first alternative is to put a central heater in one of the buildings, the home for the aged, in which we will burn oat.

Endocrine disruptive chemicals (EDCs) are found in many everyday products and can cause adverse effects on human health. Small children are particularly sensitive to EDCs and they are also exposed to them in a greater extent than adults. Despite this, EDCs are only banned in a few toys and childcare products. There are many sources of exposure and if no steps are taken to reduce the incidence of EDCs we may face an increasing number of people diagnosed with obesity, diabetes type 2 or various types of cancer.The Swedish Government has set a number of environmental objectives, one of which is calleda non-toxic environment. The Swedish Chemicals Agency was instructed to produce and conduct a national action plan to achieve this goal.

Itraconazole is a third generation azol, a fungicide which acts by inhibiting ergerosterol synthesis in the fungal cell membrane, and thereby disturbing fungal regeneration. It has proven to be less toxic, to have a broader spectrum of activity and to be more potent than its predecessor ketoconazole. Itraconazole is only available in therapheutics approved for human use. Pharmacokinetic studies has been performed in humans, dogs, cats and laboratory animals. Although itraconazole has been used tentatively with good effect against fungal infections in horses, no pharmacokinetic studies have yet been done.

To provide our company a new business opportunity I have in this essay examined the economical possibilities to sell energy. I have chosen to do a case study on my own farm in this essay. The farm is focused to cropping and also have some properties for hire. The farm is situated in the village of Fjelie and that?s where the opportunity for selling heat are. Fjelie has about forty houses. The idea is to build a plant where straw is being used as fuel.

I have always lived around mines and around people work­ing with mines. They are a natural part of my life and, inev­itably, a great interest of mine. When time came to make a decision for a degree project, focusing on the mine context felt as natural as breathing.In LKABs underground mines, safety in fire accidents is a great priority. Of outmost importance for the escape is to have a personal gas filter for filtration of smoke and/or gas. My design project resulted in a concept which is aimed at shortening the user journey from the start of the accident until the user has applied the personal safety solution and is ready to escape.Throughout the project I?ve been working with a context which is heavily regulated, and all problems are serious, important ones.

Public concern about the release of toxic compounds from wood-based products drives legislation towards enforcing industries to find and use more sustainable solutions for wood treatment. Plant oils are good alternatives to treat wood, minimizing or even neutralizing the impact on the environment. Wood modification with epoxidised linseed oil (ELO) and acetic acid as catalyst has proven a suitable method to impregnate and protect wood. However, the mixture presents a serious inconvenience because of the quick polymerization of the ingredients that limits its practical application. Therefore, this study was designed to evaluate an alternative method using a two-step process which bypassed the problem above.

The environmental work in Sweden has developed a lot over the past fifty years. Today, most companies have some kind of environmental work. Environmental activities are regulated by the Environmental Code, which applies in parallel with other laws. This report is an environmental review for Bräcke Diakoni. The report aims to find out how much environmental impact Bräcke Diakoni has.

This thesis deals with the design of a combinated rescue-, personal-, and firefighting elevator for a nuclear waste storage facility to be constructed by the client SKB, Svensk Kärnbränslehantering AB. SKB:s mission is to dispose of all nuclear waste from all swedish nuclear power plants. The storage facility will be built in the rock 450 m under the surface and will consist of several kilometers of tunnel where capsules containing the nuclear waste will be placed. It will also hold a central area which contains spaces necessary for the operation and construction of the facility, such as elevator hall, storage hall etc. The facility will have two ways of access: a vehicle ramp and an elevator shaft.

Immune stimulating complex (iscom) matrix is a formulation that is used as anadjuvant within research. The capability of the iscom matrix to elicit an immuneresponse when not present together with an antigen has not been extensivelyinvestigated. One way to evaluate the status of the immune system is to measure theamount of cytokines that usually are produced by various cell types during an immuneresponse. The aim of the present study was to evaluate the early immune responses inporcine peripheral blood mononuclear cells (PBMC) after stimulation in vitro with theiscom matrix, AbISCO®-100. The immune response was evaluated by measuring themRNA expression for various cytokines, a chemokine receptor (CXCR4) andregulator of G-protein signaling (RGS16) in porcine PBMC after stimulation withAbISCO®-100 or other inducers like a lipopolysaccharide (LPS) and a CpGoligodeoxynucleotide (ODN 2216) used as controls.

Product development is a risky business; many products never yield profit, but it is also ne-cessary for a company?s survival in a competitive business where the costumers change their requests constantly. Consumer-led product development is developed from the wish to fill the consumer?s needs and expectations and thereby be more likely to succeed. Lindell?s Gårdsslakteri AB is a local slaughter and meat processor in Västergötland.

Bipolar disorder is a severe, affective illness which causes a person to alternate between episodes of pathologically elevated mood (mania) and depression ? in between these episodes the mood is normal. The length and intervals between the episodes are highly variable among the ill, as are the severity which is divided into two major subgroups: bipolar I and II. Bipolar I disorder is the most severe because of the patient?s inability to realize that he or she is currently in a manic phase, in the case of bipolar II disorder, the patient is aware that he or she has an abnormally raised mood, a state which is called hypomanic state instead of manic.