Scrapie is an infectious neurologic disease in sheep caused by prions, corresponding to transmissible spongiform encephalopathies (TSEs) in other species. The prion is presumably constituted of PrPSc, the misfolded form of the normal endogenous prion protein, PrPC, which is found in practically all cells in the body. PrPSc can induce a conformational change in PrPC and misfold it as an imprint of itself; this is how prions amplify and spread. The process of conformational change is poorly understood and there might exist intermediate forms between PrPC and PrPSc. Bioassay using mice has traditionally been the golden standard in prion studies but is limited by cost and time.

Under en tidsperiod på tretton veckor mättes temperaturen på tvåhundratjugo suggor och etthundraåttiotre smågrisar i grisningsboxar vid SLUs forskningsanläggning. Vid varje mätningstillfälle mättes rektaltemperatur samt hudtemperatur på tryne, öra, bog, länd och vulva. Varje mätområde på huden mättes tre gånger vid ett mättillfälle, två olika IR-termometrar användes. Detta innebar totalt ettusen etthundra enskilda mätningar på suggor och niohundrafemton mätningar på smågrisar. som samlades in och analyserades avseende korrelation mellan de olika mätområdena på huden och rektaltemperaturen.

The purpose of this study is to examine what professionals tell about stress, rewards, impact and treatment in the professional treatment with young sexual offenders. Eleven qualitative interviews of half-structured design brought up results about the professional role in the treatment process. During the interviews the professionals described which methods of treatment they work with. We examined the methods through literature research, which was added to the study whit the purpose of strengthening the interview data. The professionals consider their work important and meaningful and they feel safe in their working role.

Dödfödda grisar och smågrisdödlighet under diperioden leder till både produktionsförlust och innebär dessutom försämrad djurvälfärd. Förluster orsakade av dödfödslar, smågrisar som kläms ihjäl av suggan och som svälter ihjäl kan förebyggas genom god skötsel, bra djurstallar ochavelsurval. För att lyckas med detta krävs att riskfaktorer för smågrisdödlighet kan identifieras.Syftet med den här studien är att undersöka variationen i förekomsten av dödfödda smågrisar och dödligheten under digivningsperioden beroende på kullnummer, kullstorlek, säsong och besättning. Ett andra syfte är att fastställa om det finns någon upprepbarhet för suggor vad gällerförekomst av dödfödda smågrisar. Data erhölls från fyra kommersiella grisbesättningar, belägna i centrala Sverige, som använder sig av besättningsövervakningsprogrammet ?PigWin Sugg?.

Infectious Bronchitis (IB) is a highly contagious viral respiratory and productiondepressant disease distributed world wide. The disease is caused by the InfectiousBronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within thegenus Gammacoronavirus. Due to a combination of high mutation rate and atendency of recombination a constant emergence of new genotypes and serotypesis seen. In Brazil, a country with an extensive poultry production and majorproblems with disease control, the diversity of circulating strains are wide andoutbreaks of clinical disease despite intensive use of vaccines are common. Theaim of this study was to investigate the presence of IBV and to identify genotypesof isolated IBVs in a limited number of layer flocks in Bastos, São Paulo, as partof the screening for circulating genotypes in the country.

Detection of leukocytes in semen from boars is part of the routine control of semen quality. Leukocytes in semen can cause damage to spermatozoa that leads to impaired fertilization. In the artificial insemination (AI) industry there are standard routines for the quality control of the boars and of the neat semen before use. Since 2001, Quality Genetics has not done the leukocyte examination on semen samples from boars which are used for the production of pigs for slaughter. The purpose of this study was to investigate the occurrence of leukocytes in semen from AI-boars. The questions addressed were whether there are boars that have leukocytes in their semen and how frequently it occurs and, moreover, if there are differences between breeds, or even between individuals whose semen was checked before use in AI-dose production compared to those which were not. The study included a total of 135 AI-boars, of which 67 were Hampshire, 35 Yorkshire and 33 Landrace, all belonging to Quality Genetics AI-station in Hållsta.

The search for antibiotic compounds from the natural environment has been going on for seven decades, ever since penicillin entered the market and antibiotic treatments became routine. The evolutionary pressure put on the pathogenic microorganisms induced a rapid spreading of naturally occurring resistance genes, leaving only the option of finding new antibiotics to treat the resistant pathogens. Microorganisms have been extensively mined for their biosynthetic abilities to produce biologically active compounds. To date, more than 23 000 microbial natural products have been discovered. The Actinomycetales are ubiquitous bacteria that have been used for antibiotic discovery for more than half a century, and over 10 000 natural products have been identified from the order.

Probiotics is a special group of health products on the market. The most common type of  these is live cultures of lactic acid fermenting bacteria from raw milk . These include, among others, yogurt and various trademarks such as Hälsofil ®, Acidophilus ®, Dofilus ® and Kefir ®. Such products are often used by patients who are treated with antibiotics to combat the intestinal flora eliminated, thus causing digestive problems. The present work deals with yet another type of health product, viz.

Japanese encephalitis virus (JEV) is one of the leading causes of acute encephalitis in humans. The virus is spread by mosquitoes, mainly belonging to the Culex species. The main reservoirs are considered to be birds and pigs, with pigs constituting the most important reservoir in regard to human infection. During the last few decades the production of pork has increased significantly in Vietnam. With 90% of pigs being kept in households owning 10 pigs or less, pigs are widely spread across most parts of the country.

Today the individual ventilated cage system (IVC-system) is a commonly used housing system for mice in research facilities. IVC systems have HEPA- filters and are designed to control both supply and exhausted air at cage level, as well as temperature and relative humidity in the cages. This creates an optimal environment for the mouse and at the same time a protection against the risk of cross-infection between cages and the outside environment. The aim of this study was to evaluate the health and welfare of breeding females and pups of two different strains of laboratory mice (NMRI nude mice and C57BL/6N) in three different kinds of IVC-systems; Arrowmight, Allentown and Tecniplast. The NMRI nude (Nu) females gave birth to 18 litters in Allentown, 16 litters in Arrowmight and 15 litters in Tecniplast.

Hard-to-heal ulcers are resource intensive due to the fact that they are difficult to treat and especially vulnerable to bacterial invasion. The bacterial culture contaminating these wounds often consist of several different bacterial organisms that originate from endogenous sources. Necrotic material in ischemic ulcers provide nutrition which support bacterial reproduction, increasing the risk of infection. Determining causative pathogen in infected ulcers proves to be difficult when culturing swab samples, however Staphylococcus aureus and hemolytic streptococci generally act as primary pathogens.    The aim of the study was to investigate if the detection rate increased for S. aureus and hemolytic streptococci when culturing swab samples from ulcers on Columbia CNA; a media selective for gram-positive bacteria.

The preschool constitutes a big part of many children's everyday existence. Its contents are therefore of great importance and the environment deserves a well thoughtout design. In this study, surveys in field and literature have resulted in conclusions around how plants can be used in children's play environments, in order to contribute to the experiencerelated values. The aim is to present conclusions to lean on when drawing up designproposals for new playgrounds, especially where the natural conditions are not so favorable. The first question that is responded to is how plants can be used in order to enrich the children's play environments.

Pyometra is a common and life threatening disease of intact bitches. The disease is caused by a hormonal influence on the uterus in combination with a bacterial infection. The most common treatment is ovariohysterectomy, but several medical options are available to maintain fertility or avoid surgery and anaesthesia. Drugs that can be used for medical treatment are for example progesterone-receptor antagonists, prostaglandins and dopamine agonist. The present study focused on treatment with the progesterone-receptor antagonistaglepristone in combination with antimicrobial therapy.

It is important to investigate the potential of biological control measures in agricul-ture, especially where economic issues restrict the use of expensive inputs or when there are environmental concerns about toxicity of agrochemicals. The bacterium Bacillus subtilis has proved promising as a biocontrol agent (BCA) in suppressing various plant diseases and it has also been shown to promote plant growth in certain cases. During this study, the effect of B. subtilis on Fusarium wilt (caused by the fungus Fusarium oxysporum) on tomatoes, as well as its effect on the earthworm species Pontoscolex corethrurus was investigated. Furthermore, the combined ef-fect of the BCA with P.

The purpose with this thesis is to get a compilation of all the critical bolted joints of all enginesproduced at LV Motors. ABB LV Motors produces induction engines with seven shaft heights. Eachengine size exists several variants of components ex. standard shield and flange shield. Depending onwhich components the engine is equipped with arises various types of strains in the engine's parts,certain assembly ways are more favourable than other.The most critical parts in the engine from a mechanical point of view are the screw units betweenshield/stator, feet/stator and bearing cover/shield.