BACKGROUND: The isolation facility is an important ward in veterinary hospitals. This is where infectious patients are treated in order to reduce the risk for cross-contamination between the infectious patients and other patients and humans in the hospital. But it can only function in a safe manner as long as adequate precautions for infection control are adhered to. This has shown to be a problem in many isolation facilities in veterinary hospitals in Sweden. AIM: The aim of this paper was to examine which factors that can affect the quality of the infection control in the isolation facility. The aim is also to stress the importance of adequate infection control practices. METHOD: Two interviews were conducted with staff from two veterinary hospitals.

AbstraktBakgrund: Länge har man varit medveten om vikten av god hygien och dess inverkan påsmittspridning. Det mest effektiva sättet att minska postoperativa sårinfektioner (SSI) är att arbetaförebyggande och att ha en god hygien. Frekvensen av SSI ökar, och som en del av den förebyggandevården ingår den preoperativa förberedelsen. Att inte ha kunskap och stöd till att utföra en sådanåtgärd korrekt är en stor brist och kan leda till att patienter drabbas av SSI och därmed ett ökatpostoperativt lidande.Syfte: Studiens syfte var att beskriva vårdpersonalens syn på betydelsen av hygienisk preoperativförberedelse hos ortopedpatienter.Metod: Studien genomfördes på ett länssjukhus i mellersta norrland under våren 2008, där allaortopedavdelningar på sjukhuset deltog. En kvalitativ intervjumetod användes med manifestinnehållsanalys av materialet.Resultat: All personal som ingått i studien anser att den hygieniska preoperativa förberedelsen är avstor vikt.

During the last years, meticillin resistant Staphylococcus pseudintermedius (MRSP) have caused an increasing number of infections in Swedish dogs. MRSP is resistant against a majority of the antimicrobials available for dogs in Sweden, making it difficult to treat the infection. Our knowledge of the bacterium is limited, making it hard to handle infected dogs in a proper way. The aim of this study was to investigate for how long time dogs, who have an infection caused by MRSP, become carriers of the bacterium. Other factors like treatment with antimicrobials, the correlation between clinical symptoms and carriage, common diagnoses and institutionalization where also looked upon.

Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence assay, mouse inoculation and RT-PCR. The sources of infection were determined by sequencing 234 nucleotides of the 5? end of the N-gene and align these with homologous sequences retrieved from GenBank.

Alla patienter som genomgår någon typ av operation där man penetrerar huden har en ökad risk att drabbas av en postoperativ sårinfektion. Syftet var att utforska de preoperativa åtgärderna huddesinfektion och hårborttagning relaterat till uppkomsten av postoperativa sårinfektioner. Metoden var en systematisk litteratur studie där nio artiklar användes som underlag. Resultatet visade att klorhexidin reducerar bakterierna på huden, men ingen signifikant minskning av antalet postoperativa sårinfektioner kunde påvisas. Av hårborttagningsmetoderna rakhyvel, rakapparat och hårborttagningskräm ger rakhyvel upphov till fler små sår..

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Syftet med denna litteraturstudie var att beskriva varför följsamheten gällande handhygien ofta är låg bland vårdpersonal, samt hur man kan öka följsamheten för handhygien bland vårdpersonal. Metoden som använts var en litteraturstudie. Datainsamling skedde via databaserna Medline (PubMed), Cinahl (EBSCO host) och Academic Search Elite. Sökorden som använts: prevention, hand disinfection, hand washing, infection control, nosocomial infection och education. Totalt användes 15 vetenskapliga artiklar.

This report includes common helminths, symtoms, routes of infection, diagnostic laboratory techniques and prophylactic methods to prevent infection. We have pointed out where there is a risk of zoonotic transmission and how to avoid contamination and infection. We have restricted the number of species to the most common helminths. Some of these helminths do not naturally occur in Sweden (yet) but are nevertheless of current interest when we travel across borders with our horses, dogs and cats. The diagnostic part of our report tells about different techniques to find an occurance of helminths in samples of faeces, blood, urine or saliva from a horse, dog or cat. We also write about some recommendations on treating parasitic infections and the development of anthelmintic resistant helminths that originates from incorrect use of anthelmintic drugs .

Lactobacillus reuteri has been used as probiotics in piglet and chicken feedings as well as in different diary products for humans. In this EEF-work I have shown that L. reuteri can be an alternative for treatment of vaginitis in laboratory rats used for breeding. In a breeding stock with 3000 animals some animals had a vaginal infection with ??haemolytic streptococci group G.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

ABSTRACT The aim of this thesis is to illustrate how surgical operation theatre nurses perceive debriefing within emergency health care. The study has a qualitative design with semi structured interviews that have been performed with seven operating room nurses working in emergency health care. The strategic selection consists of professional operation theatre nurses working in emergency care at Uppsala University Hospital. The main goal of this thesis is to increase the understanding of debriefing and to highlight the need of debriefing after difficult or unexpected events in the emergency health care profession.The interviewed nurses perceive debriefing as an important way to develop the day to day work, but also as a way to unburden the staff at the time of bigger traumas. Even though the effect of debriefing is not univocally positive in the literature, the staff perceives that it reduces different kinds of stress.

The aim was to describe the experiences of couples where the male partner had undergone radical prostatectomy due to prostate cancer. Furthermore, the study explored how the operation had affected their sexual life, whether the couples had received enough post surgical support and information concerning these issues, and to describe the kind of support and information these couples desired. Twenty men and ten partners participated. All the men experienced a varying degree of post surgical erectile dysfunction and only five men had received support concerning their sexual life. Eleven men desired professional visiting support activities.

Background: Surgical complications has been the main cause of medical injuries and deaths worldwide. In 2008 WHO developed the checklist for safe surgery to reduce the number of surgical complications. The objective of the checklist is to strengthen already established safety routines and contribute to better cooperation and communication. Research shows that compliance to the checklist is deficient, which can affect patient safety.Aim: The aim was to study the extent to which theatre nurses participate in the use of the checklist.Method: Observational study was conducted with a descriptive design and quantitative approach. 24 observations were carried out with aid of an observation protocol of two surgical units at a hospital in central Sweden.Result: The results showed that Timeout initiated widely but compliance to all items was considerably lower.

Bovine Respiratory Syncytial Virus (BRSV) is a common cause of respiratory disease among young cattle. The virus causes severe losses; the herd mortality rate can sometimes be as high as 20 %. In this study the avidity (i.e the antigen binding force) of BRSV specific antibodies was measured to see if there was a difference between antibodies produced during an acute phase of infection and antibodies produced by earlier infected animals. A commercially available ELISA-testkit against BRSV-specific antibodies was used and an incubation step with 6M urea was added. The effect of the urea is that it breaks the weak bonds between antibodies and antigen while the stronger bonds remain intact. Four different groups of animals were included in this study; seven calves that were naturally infected, three acutely infected calves with known time of infection, five cows that were seropositive during several years and four experimentally infected calves that had been a part of a vaccine trial. The results of this study showed that antibodies produced during the acute phase of an infection had a low avidity and that the avidity increased with time after infection.