Det kommer ständigt nya studier med olika hälsoråd angående kosten och fysiskaktivitet om hur vi ska undvika fetma, högt blodtryck samt hjärt- och kärlsjukdomar.Intresset av proteinets effekt på uppbyggnad av muskelmassa vid fysisk aktivitet harökat. Detta har medfört ett stort urval av olika drycker med proteintillskott påmarknaden, främst ur animaliskt ursprung.Syftet med detta examensarbete var att ta fram en vegetabilisk proteinberikadhavredryck som förväntas hjälpa kroppen vid återhämtning. Arbetet innebar att utvecklaett recept baserat på de Nordiska Näringsrekommendationer 2004 (NNR) med fokus påprotein, kolhydrater och fett. Delmålet var även att utesluta allergener. Dryckenbaserades på ett medelvärde från de fysiskt aktiva och äldre människors näringsbehov,detta för att bredda den tilltänkta dryckens målgrupp.Fyra smaksatta drycker framställdes med olika energiinnehåll utifrån två olikahavredrycker, en naturell och en kalciumberikad.

Horses are grazers with a digestive system that is adapted for a high fiber diet. Forage is a fiber rich feed that includes grass and legumes. A deficiency of forage and an excess of grains may cause gut disturbances and stereotypic behavior. The aim of this literature study was to summarize information on the horse?s capacity to consume forage-only diets with focus on the horse?s need of energy and protein.

Sahlgrenska Academy at University of GothenburgDepartment of Internal Medicine and Clinical Nutrition3AbstractTitle: How does isoflavones in combination with soy protein impact cholesterol levels in hypercholesterolemic postmenopausal women relative to milk protein?Author: Emma Edberg och Emma NilssonSupervisor: Lena HulthénExaminer: Anna WinkvistProgramme: Dietician stydy programme,180/240 hpType of paper: Examination paper, 15 hpDate: 2014-05-26Background: Hypercholesterolemia is a major risk factor for cardiovascular disease and the number one cause of death among women in Western countries. Declining levels of estrogen at menopause could be a contribution factor. Isoflavones is a vegetable substance, with structural and functional similarities to the human estrogen. This is a potential reason for possible cholesterol-lowering effects.

The coproduct, dried distillers grains + solubles (DDGS) from ethanol production based on wheat contains much rumen degradable protein and fiber with low digestibility. To compliment this quality in an effective way a grass silage low in protein and much digestible fiber is needed. The aim of the study was to investigate the effects of adjusting the crude-protein concentration and fiber quality of the silage when feeding DDGS and of varying the inclusion level of DDGS, when feeding the adjusted grass silage, on intake, milk yield and composition, faecal traits and feed efficiency. Four different total mixed rations were compared. The normal grass-clover silage had a crude protein content of 17,8 % and an NDF content of 46,2 % of dry matter. The adapted grass silage had a crude protein content of 14,6 % and an NDF content of 52,9 % of dry matter. The diets were formulated to have similar nutritional content except for A + 4 which had a higher content of rumen degradable crude protein and a lower concentration of NDF.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

Sulforafan är en isothiocyanat-förening som finns i stora mängder i olika kålväxter. Sulforafan har setts ha en kemopreventiv inverkan bland annat genom att inducera fas II-enzymer styrda av transkriptionsfaktorn nuclear factor E2-related factor-2 (Nrf2). Nrf2 är mycket viktig i cellers skydd mor oxidativ- och elektrofil stress och inducerar uttrycket av många olika gener, bland annat olika tranportproteiner. Breast cancer resistance protein (BCRP) är en av transportörerna som tros induceras av Nrf2. BCRP finns i stora mängder i lakterande juvervävnad men också i många andra vävnader där den transporterar läkemedel ut ur cellerna.

Arbetet beskriver en analys av innehållet av membranbundna och periplasmiska c-cytokromer hos perkloratodlade GR-1 och jämförelser med c-cytokrominnehållet hos Ideonella dechloratans och andra kända c-cytokromer, samt med genomet för Dechloromonas aromatica. Den jämförande studien av c-cytokromer gjordes med syftet att undersöka en hypotes om att bakterierna använder olika vägar för elektronöverföring till det periplasmiska enzymet (per)kloratreduktas. Cellmembran från GR-1 renframställdes genom ultracentrifugering och periplasma preparerades fram med hjälp av osmotisk chock. Fraktionerna analyserades sedan med SDS-PAGE och peptider med kovalent bundet hem (c-cytokromer) detekterades med hjälp av en specifik färgreaktion. Även Touchdown PCR med degenererade primrar genomfördes på isolerat DNA från GR-1 i ett försök att finna en gen kodande för ett NapC/NirT-liknande protein.

Bovin spongiform encephalopathy (BSE) är en sjukdom som drabbar nötkreatur, och varssmittämne är det ofullständigt utforskade prionproteinet. Detta protein finns i vår egen kropp,men då veckat till strukturen av en alfahelix istället för ett betaflak. När vi får i oss ettfelveckat protein verkar det som att en domino-effekt sker och alla normala proteiner avdenna sort veckar sig fel och ansamlas i centrala nervsystemet (CNS). Människor som ätersmittat kött kan drabbas av den humana formen, variant Creutzfeldt-Jakobs sjukdom (vCJD),som är en neurodegenerativ sjukdom med lång inkubationstid och som oavkortat leder tilldöden. I denna litteraturstudie görs ett försök att belysa dessa sjukdomar och problematikenrunt omkring, med betoning på BSE.

AbstractAn approaching overproduction of cereals and a future uncertain supply of non genetic modified vegetable protein feedstuffs have increased the interest in an expansion of the domestic production of protein feedstuffs in Norway. Besides rapeseed (Brassica campestris), peas (Pisum sativum) may be the most suitable crop for this purpose. Peas are characterized by having a relatively high content of crude protein, ranging between 20-26%, a high content of starch, 42-51%, and a low content of fat. In general, the energy value for peas is higher than for barley but lower than for rapeseed and soybean meal.Pea protein consists of albumins and globulins to 85-100%, which leads to the fact that a large part of the pea protein is soluble and degradable in the rumen. Starch in peas is on the other hand to a large extent resistant to rumen degradation compared to starch from other starch rich feedstuffs.

Working horses in developing countries are mostly used for transportation. They are often owned by people with limited economical resources and lack of knowledge about how to take care of the horses and due to this the horses often suffer from problems such as dehydration, poor body condition, lesions and parasitic infections. The aim of this master thesis was to investigate if free veterinary care improved the health and hydration status of working horses in Nicaragua and it was performed as a part of a project called ?Free veterinary assistance for working horses and training of their owners as veterinary promoters in the Pacific coast of Nicaragua?. Five parameters were used to investigate whether free care improved the health of working horses in six towns along the Pacific Coast of Nicaragua.

In the spring of 2006 we had our first outbreak of avian influenza of highly pathogenic H5N1 (HPAI) among wild birds in Sweden. This disease have impact on poultry industries world wide. Sporadically the virus infects other species than birds and we now know it can even cause fatal infection of humans. There are not yet any confirmed cases of transmission of the disease between humans though a suspected case in an Indonesian family is under investigation. If a mutation occurs that changes the pathogenicity and host affinity to humans we are at a risk of a new pandemia, perhaps like the Spanish flu in 1918-1920.

Protein i foder utnyttjas av idisslaren via upptag av mikrobprotein i tunntarmen eller som foderprotein som passerat vommen vidare till tunntarmen. Olika råproteinnivåer i foder till idisslare påverkar både miljön och djurens avkastning. Råprotein från vallväxter kan analytiskt delas upp i olika fraktioner utefter var i idisslaren det bryts ned och kan tillgodogöras. Ett sådant system är The Cornell Net Carbohydrate and Protein System (CNCPS) vilket delar in protein i fraktionerna A, B och C utefter deras nedbrytningshastighet i vommen. Många olika faktorer kan påverka halt och sammansättning av råprotein i vallfoder.

Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.