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Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden
The
focus
of
this
project
was
to
characterize
the
occurrence
of
ESBL-?producing
bacteria
in
two
irrigation
water
ponds
in
Southern
Sweden.
Samples
were
taken
from
two
ponds
nearby
each
other,
from
which
analyses
were
made
based
on
levels
of
community
and
individual
isolates.
Community
samples
were
used
for
attempts
for
characterization
of
resistance
patterns
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade.
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade..
Mikroproducenternas påverkan på Elinorr
The Swedish energy system will be influenced by many future changes, such as energy and climate policy objectives. The installation of more renewable electricity could increase by using solar energy. Over the recent years, grid-connected photovoltaic (PV) systems have increased dramatically in Sweden, partly due to various support systems and compensation models. These support systems have been designed to make it easy for individuals and businesses to invest in self-generated electricity and thus contribute to the transformation of the energy system.The introduction of a higher proportion of solar energy may in turn affect the Swedish electricity system. This could mean that the electricity companies will be facing a number of changes in the near future.
Barnens budget : En studie av Växjö kommuns projekt i demokratisk skolning
Young people?s political influence has been surveyed, studied and discussed several times in Sweden during the last decade. There is a fear among the politicians that the young people are not interested in politics, especially not in party politics. On the other hand there is, among the youngsters a feeling of invisibility, not being seen or heard or for that sake respected. There are a lot of formal possibilities for young people?s political influence, but there are not as many real possibilities or situations to actually practice these possibilities.
Design och utveckling av monteringsverktyg
With this essay we want to describe the transformation process that the real estate owner has to go through to sell the current rented flats to tenant. We also want to provide an understanding how the valuation is performed in the transformation process and highlight the issues that affects the price in the valuations. In the essay we would like to see if the valuation and the transformation process differ in a public real estate company compared to a private real estate company. Based on this we have asked us the following question to answer: -Is there any differences in how real estate?s are valued in the transformation process between public real estate companies and private real estate companies? We have used a qualitative approach to keep our main question.
Hainan. En kinesisk destination för nordiska turister?
The purpose of this paper is to describe and analyse the Chinese destination of Hainan, with background in a globalizing tourism destination development, for tourism from Finland and Sweden. The questions at issue are regarding what kind of an impact the status as a special economic zone have had upon Hainan, what kind of conditions there are for tourism on the island, in what form participants of the tourism industry want the development to proceed, what the prevailing trend is for distant destinations for Nordic markets and also regarding what kind of potential there is for organized tourism to Hainan from Finland and Sweden.As a foundation for the paper, I?ve composed a theoretical reference for knowledge of China, Hainan and the study of destinations. The sources of secondary kind are literature related to the subject. The primary sources are mainly interviews, field work done at Hainan has given a concrete and personal knowledge of the place.
Attitudes towards hedging by diversified and non-diversified farmers : a comparative qualitative study
Deregulation on the market for agricultural products leads to a more globalised market with increasing price fluctuations. This, in turn, places the farmer in positions influenced by new risks but also improved opportunities. The farmers are faced by uncertainty in terms of financial outcome. To be able to utilise these new market conditions it becomes increasingly important for farmers to continuously follow the price trend, and to develop strategies how to manage the risks exposed by a volatile market.
Farmers with different conditions perceive risk in different ways. Hence, their risk management behaviour will vary.
Vårdberoende, behov av omvårdnad och insatser för äldre med regelbunden vård och omsorg ? del av SNAC-Blekinge, Vårdsystemdelen
Background: The relationship between advanced age, presence of illness and impaired functioning is well known. A large proportion of the elderly population has an extensive need of care and service and therefore need help from municipal care. Aging is a transition in life and also affects the person's identity and self image, making the person particularly vulnerable and challenging everyday safety. It is therefore of importance to gain knowledge about which factors at individual and organizational level that support a person-centered nursing care for the elderly so that caring responsibilities and staffing of nursing personnel may be scheduled based on need. Aim: This study aimed at describing dependency and care needs of elderly persons living in ordinary housing, sheltered housing and nursing homes.
S:t Göransområdet : gestaltningsprogram för ett stadsutvecklingsområde på nordvästra Kungsholmen i Stockholm
North Western Kungsholmen in Stockholm is confronting big changes and Stockholmsstad is planning that the area will be an extended part of the existing structure in centralStockholm. Dwellings and offi ces are built and the population increase heavily. Th isresult in a need to look at how public green areas can develop and be designed. Th epurpose is to design a proposal for a master plan of S:t Göranområdets public greenareas. Exploateringskontoret, Stockholm stad, who has initialized the task, claim thatthere is a need of clarifying and shield the qualities in the green areas of today and howto create new green structure.The problem formulations which are current in this master thesis are:? Is there a need of a distinct identity for the green areas of S:t Göransområdet? If so,how is this created?? How can the green parks and nature areas inside S:t Göransområdet be improved anddevelop?? How can the connections between the green areas inside S:t Göransområdet beclarifi ed and how can they connect to the surroundings?To get inspiration and information for the design this thesis include a study of theincluding parts in the design process.
Vemmenhögsprojektet : ett framgångsrikt sätt att minska läckage av bekämpningsmedel från odlad mark
Det är mycket viktigt att förhindra att bekämpningsmedelsubstanser läcker ut i miljön
och orsakar skada. Lagstiftningen kräver därför att läckaget av bekämpningsmedel från odlad mark minskas. Olika myndigheter och organisationer har olika ansvarsområden
beträffande bekämpningsmedel. Bekämpningsmedelssubstanser kan, om de inte hanteras rätt, vara skadliga och därför finns det tydliga bestämmelser om hur dessa substanser ska
hanteras.
Många oberoende undersökningar visar tydligt på att det förekommer läckage av bekämpningsmedel i samband med att de används i behandlingar av odlad mark. Undersökningar har pågått under lång tid både i Sverige och i andra länder.
Ett flertal åtgärder och metoder mot läckage av bekämpningsmedel från odlad mark har framtagits och utvecklats som radikalt kan minska läckaget av bekämpningsmedel.
OMBILDNING FRÅN HYRESRÄTT TILL BOSTADSRÄTT : En jämförelse mellan ett allmännyttigt och ett privat fastighetsföretag
With this essay we want to describe the transformation process that the real estate owner has to go through to sell the current rented flats to tenant. We also want to provide an understanding how the valuation is performed in the transformation process and highlight the issues that affects the price in the valuations. In the essay we would like to see if the valuation and the transformation process differ in a public real estate company compared to a private real estate company. Based on this we have asked us the following question to answer: -Is there any differences in how real estate?s are valued in the transformation process between public real estate companies and private real estate companies? We have used a qualitative approach to keep our main question.
Rötning av industrihampa förfiberproduktion : en utvärdering av kvalité och miljöpåverkan
North Western Kungsholmen in Stockholm is confronting big changes and Stockholmsstad is planning that the area will be an extended part of the existing structure in centralStockholm. Dwellings and offi ces are built and the population increase heavily. Th isresult in a need to look at how public green areas can develop and be designed. Th epurpose is to design a proposal for a master plan of S:t Göranområdets public greenareas. Exploateringskontoret, Stockholm stad, who has initialized the task, claim thatthere is a need of clarifying and shield the qualities in the green areas of today and howto create new green structure.The problem formulations which are current in this master thesis are:? Is there a need of a distinct identity for the green areas of S:t Göransområdet? If so,how is this created?? How can the green parks and nature areas inside S:t Göransområdet be improved anddevelop?? How can the connections between the green areas inside S:t Göransområdet beclarifi ed and how can they connect to the surroundings?To get inspiration and information for the design this thesis include a study of theincluding parts in the design process.
Välfärdseffekter av ett frihandelsavtal : en ekonomisk analys av ett EPA-avtal mellan EU och ESA
The EU has had a special agreement with their former colonies in Africa, the Caribbean and the Pacific Islands, the ACP-countries, for almost 30 years. This has granted the ACPs with preferences on the Europen market, which have been excluded from other countries. The agreement is now beeing re-negotiated to make it more in terms with the rule of the WTO?s most favoured nation-principle. The new agreement will differ from the present one as it will be a mutual free trade agreement where the ACP countries will open their markets to the EU as much as the EU opens up it?s market to them.
Beräkning av optimal batchstorlek på gavelspikningslinjer hos Vida Packaging i Hestra :
This final thesis aim to create a support that can decrease the uncertainty when planning the cable drum flanges lines that produces cable drums in the interval 100 to 320cm in diameter at Vida Packaging in Hestra. The purpose attains through finding an optimal batch size that minimizes the total storage cost and order prime cost, without degenerate delivery capacity to the customer.
Through survey the sale and stock balance development during 2005 and 2006 for cable drums in Hestra and give alternative suggestions how to plan the production after installing a new cable drum flanges line. The study also explores different ways of working during product change in existing nailing line, to minimize the order prime cost.
Vida Packaging in Hestra is a part of the Vida group, which is Sweden?s largest privately owned sawmill group with seven sawmills placed all around southern Sweden.
Hur bokskogslagen blev till ? konflikter och lösningar 1964-1974
During the early Middle Ages beech forests (Fagus sylvatica L.) spread out over large parts of southern Sweden. They had a crucial role in human survival for hundreds of years. For various reasons, the beech forest area has decreased since the 1600s. Spruce planted on former beech forest land was the primary reason for the decline during the second half of the 1900s, which made many worried about the future of the beech forests and led to a beech forest act in 1974. This study describes the background to the beech forest act based on parliamentary official documents.
Vårdberoende, behov av omvårdnad och insatser för äldre med regelbunden vård och omsorg ? del av SNAC-Blekinge, Vårdsystemdelen
Background: The relationship between advanced age, presence of illness and
impaired functioning is well known. A large proportion of the elderly
population has an extensive need of care and service and therefore need help
from municipal care.
Aging is a transition in life and also affects the person's identity and self
image, making the person particularly vulnerable and challenging everyday
safety. It is therefore of importance to gain knowledge about which factors at
individual and organizational level that support a person-centered nursing care
for the elderly so that caring responsibilities and staffing of nursing
personnel may be scheduled based on need. Aim: This study aimed at describing
dependency and care needs of elderly persons living in ordinary housing,
sheltered housing and nursing homes. Furthermore the study aimed at describing
how the dependency was related to the staffing of non-licensed and licensed
nursing staff in nursing homes.
Method: A descriptive and analytical quantitative method was used in the study.