Flowcytometric technology has been widely used for measurement of the absolute numbers of T-lymphocytes subsets in Human Immunodeficiency virus (HIV), defining the disease state, monitoring antiviral treatment, and identifying any risk for opportunistic infections. A manual preparing of the samples is required. More recently an automated and enclosed blood cell counting, Celldyn Sapphire has been introduced. In this study the performance of the Flow cytometer EPICS XL as a reference method for analysis of CD3+, CD4+ and CD8 T-lymphocytes was evaluated with blood from 40 individual?s samples.

Brämhults Juice AB is one of Sweden?s leading producers of fresh fruit juices and drinks. The fruit drinks last 3 days once opened and when stored in a refrigerator kept at 7°C. Since factors that shorten the drinks? best before date are usually not noticed until the entire batch has expired, it would be advantageous to find a way to accelerate the drinks? natural deterioration, so that an unusal short best before date is noticed before more products are shipped off to the stores.

The calcium homeostasis in the body is normally strict regulated to be kept within very narrow limits. This regulation is due to the action of parathyroid hormone (PTH) which is synthesized in the chief cells of the parathyroid glands. In different diseases, the calcium homeostasis is disturbed and this could either be due to a dysfunction of the parathyroid gland itself, or it could have a completely different explanation. To investigate disturbed calciumhomeostasis, ionized calcium and PTH can be analyzed from a blood sample. There are a lot of different assays to measure PTH in humans.

The design of the building envelope affects the energy consumption of a building. As the energy requirements for new buildings are becoming more demanding, the performance of the building envelope must be improved. Energy efficiency and durability are important attributes to consider.This report includes investigations of the building envelope, moisture and passive solar energy. It is based on the analysis of two different buildings with different designs. These buildings stand as reference for two of the companies who are working on a new, climate smart, residential area in Uppsala, Sweden.Studies and analyses on energy consumption and moisture transportation were made.

Norconsult AB has developed a solarhousing concept, a house designed for the warm climate in the Middle East andwith large quantities of solar panels installed. The cooling system for the house was designed in an earlier report, the purpose of this thesis is to investigate the possibility of short and long term storage of thermal energy via an underground energy storage volume.Two different designs of the storage and three different filling materials have been integrated into a model to simulate different cases. The first design consisted of pipes installed in the ground, without insulation. The second design consisted of an insulated concrete box installed to prevent thermal energy from the surrounding soil to flow towards the lower temperature regions within the storage. The three different filling materials were dry saudi sand, water saturated saudi sand and the filling material used by the client for energy storage in Sweden.The results from the simulations show that neither of the designs, regardless of the filling material, managed to extract enough thermal energy from the house to the ground to uphold the demands of indoor climate.

The E-department at GETRAG All Wheel Drive in Köping have problems with the efficiency on parts of their production equipment. The equipment with the most significant efficiency problems are four automated multifunctional production cells that produces housing for rear drive units. The major part of the problem is the large amount of small stops that causes the low efficiency.The department already worked with logging of interruptions and efficiency calculations before this project started. However this work was not done to the extent considered necessary for a systematic follow-up of the interruptions.The aim of this paper is to point out a more effective and systematic way to work with logging of interruptions and the follow-up. In addition the equipment were studied with a method for efficiency calculation that ? if used properly- is a more powerful and a visually better method than the one used by the company today.As the initial current state analysis of the equipment was made a lack of documented knowledge about the equipment was discovered.

Hematology-instruments that analyze blood cells can at specific cases be followed by manual counting with a hemocytometer. The plastic hemocytometer which has a fixed coverslip and is assigned for disposable use, appears to reduce the problems arising from the use of the traditional glass hemocytometer, for instance coverslip-application difficulty and dust particles. Capillary whole blood sampling may be useful for patients at the emergency department and in addition, a smaller volume of blood is taken compared to venous blood sampling. This study will focus on the platelet- and leukocyte count (PLT and WBC) in venous and capillary blood, in order to compare the plastic- and glass hemocytometers with Sysmex XN-2000 (Sysmex Corporation, Kobe, Japan), and verify if capillary whole blood sampling can be used for the measurement of these hematological parameters. 30 subjects donated both capillary- and venous blood samples and these blood samples were analyzed with glass- and plastic hemocytometers with Sysmex XN-2000 as the reference method.

The Swedish government has introduced a goal to reduce the energy intensity by 20% to the year 2020. To reach this goal actions need to be taken throughout the energy sector which includes residential and commercial buildings. This thesis considers the possibility to reduce the energy usage in a building located close to Uppsala. The analyzed building is a combined office, cafeteria and entrance and was completed about half a year before this project started. Therefore the measures presented for a more energy efficient building are presented as measures for future constructions.Simulations have been made in VIP-Energy, a dynamic energy calculation program.

Orust Kretsloppsakademi is a nonprofit organisation, with a goal of annually generate the same amount of electrical energy that is consumed. Orust is an island on the west coast of Sweden, north of Gothenburg. The population of 15 000 reaches 40 000 during summer, due to the large amount of vacationer. The total electricity demand is 174 GWh a year with a maximum power need of 32 MW. This report presents an analysis for the plausibility of their plan and from a power system stability perspective, the effects on the electrical grid.The conclusion is that the restraints for development of power generation from wind, solar and wave generation, are the power systems capability to transfer power, primarily in the transformers of the grid.

The main interest of this study was to investigate whether there is hyperglycemia or hypoglycemia in cows suffering from abomasal displacement and to evaluate the effect of transport on blood glucose. Thirty-six cows with left- or right-sided abomasal displacement were included in the study together with 17 cows with other diseases for comparison. Fifteen cows with abomasal displacement and 7 cows with other diseases were sampled before transport. One third of the cows with abomasal displacement had hyperglycemia when the transport effects were corrected for. Separating this group in left- and right-sided abomasal displacement we found significantly higher blood glucose and insulin levels among cows with right-sided abomasal displacement. An explanation for this difference may be a strong stress response secondary to the right-sided dislocation.

This essay includes the planning and designing of a house as well as writing a rapport about howto increase fire safety in small buildings.More than 6000 homes burn in Sweden every year (Brandskyddsföreningen, 2012c). Thedevastation and the costs are usually very high. This report examines possible measures to reduceboth personal injuries and economic losses resulting from house fires in small buildings.Swedishbuilding regulationsplay a major role when it comes to constructional fire safety. However therequirements inSwedish building regulations are very low for small buildings and this is anargument for raising the requirements.The report shows and describes possible methods to build safer with regards to fire in terms ofmaterials and different building structures. Major focus has been on fire cells but other solutionsare also examined and described.

Environmental impact and management of energy is a highly topical issue in today's society. Climate impact must be reduced. The Government has set a target to reduce energy consumption in buildings by 20 % by 2020 and 50 % by 2050 compared to the energy use in 1995.With this in mind, we chose to examine how property owners can work on improving energy efficiency in existing apartment buildings. We also wanted to investigate profitability of different increasing energy efficiency measures and the calculation methods and the risk assessment performed by such investments. To tie in with government objectives, we also saw the possibilities of achieving these goals.20 companies were interviewed, of which 14 were property owners and 6 were consulting firms that works with energy efficiency.

The purpose of this essay was to review the literature and describe the patophysiology, diagnosis, microscopic and macroscopic appearance of aortic body tumors in dogs. The tumors originate from the chemoreceptor organs situated at the base of the heart. The chemoreceptor organs are neuroendocrine cells responsible for surveillance of changes in e.g. the blood pH. Aortic body tumors are rare and represent 7 % of the total cases of primary cardiac tumors in canines.

AbstractMotor sports have been around as long as motor vehicles have been. Sports are often seen as costly polluter but in the same time the motor sports have encourages a large part of development, both for vehicles and other industries. Inventions like rear mirror and ABS brakes come from motor sports. One way to once again drive development forward while making motor sports carbon-neutral is to run the race cars on hydrogen. In this thesis, the goal is to convert a spark ignition engine for hydrogen operation.

Malignant melanoma represents 4-7% of all canine neoplasms and they are the most common malignant tumors of the oral cavity and digits. These tumors are often aggressive and metastasize quickly. To date there is no effective treatment and survival times are short. Most dogs are euthanized due to complications caused by metastases. Melanin pigment is a hallmark of melanomas and is usually present in large amounts in these tumors.