Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

Perfluorinated substances (PF) are environmental pollutants that are used to coat fibres of so called ?all weather clothes? to make them water resistant. PF are effectively spread through water. Waste water treatment plants (WWTPs) are a pollutant source of these substances. PF are poisonous to different kinds of organisms, and supposedly also to human beings, if concentrations are sufficiently high.Perfluorinated substances have been investigated at three waste water treatment plants in Stockholm during the 2000?s.

Society has depended on fossil-based fuel for a long time, which has led to a fundamental increase of greenhouse gases in the Earth?s atmosphere. To tackle this problem the international community has agreed that action is necessary. Focus is now directed towards local extraction of energy from renewable sources such as biogas.Utö is participating in the Green Island project, which aims to investigate the possibility of making the island climate neutral. This report has been implemented on behalf of Skärgårdsstiftelsen, the main partner responsible for the Green Island project in Sweden.

Sewage sludge contains plant nutrients and has earlier been utilised as fertilizer to cropland. Unfortunately not only plant nutrients but also heavy metals, pathogens and organic contaminants tend to accumulate in sludge. The suitability to use sewage sludge in agriculture has therefore been subject to several discussions over the years. The Swedish food industry does no longer accept sludge as fertiliser for Swedish producers. In 1999 the Swedish parliament accepted several objectives for environmental protection. In order to prevent environmental pollution and further exploitation of the earth crust, one of these objectives confirm that phosphorous from sewage shall be recirculated to arable land.

Chemical substances are spread diffusely and uncontrollably as a result of the increased consumption of goods. REVAQ is a certification system that works to reduce the flow of hazardous substances to Swedish treatment plants and to create a sustainable nutrient recycling. According to REVAQ, the yearly accumulating rate of metals should not exceed 0.2% in soil per year. Silver and bismuth are two metals that do not fulfill this requirement. The silver concentration in sludge decreased during the last decade, however the last few years, it has leveled off.

Käppala wastewater treatment plant situated on the island of Lidingö northeast of Stockholm is running a project during 2004 and 2005 with the purpose to map out the capacity of anaerobic digestion in the digesters that treat primary and excess sludge. The purpose of this thesis work, which is part of that project, was to characterize the present anaerobic digestion process and to investigate its capacity to treat other organic wastes such as restaurant waste and waste from water works. To decide the potential of both methane and biogas production from different substrates batch laboratory tests were carried out. To imitate the anaerobic digestion process at Käppala continuous tests with small scale reactors were carried out. These reactors were later fed with restaurant waste.The batch laboratory tests showed that primary sludge had a potential biogas and methane production of 0,62 and 0,35 Ndm3/g VS respectively after 40 days of digestion.

Because of the increasing trends in energy consumption and increased environmental awareness, greater focus has been placed on improvement and development of renewable energy sources. An already proven and accepted method is biogas production from anaerobic digestion at municipal wastewater treatment plants.In the waste water treatment process solid material and dissolved pollutants are separated from the water, forming a sludge. The sludge is separated from the process and stabilized during anaerobic digestion or aerobic aeration. Most often, mesophilic anaerobic digestion is used. Because of degradation by microorganisms, biogas with a high content of methane is formed during the digestion.

The aim of this study was to in laboratory experiments investigate how addition of urea, ECOX and structural materials can optimize sanitization during composting of sewage sludge. Today we strive for a sustainable society and the importance of closing the nutrient loop increases. It has therefore become even more important to safely reuse plant nutrients from human excreta to agricultural land. Partly because it increases the sustainability of society as the world's supply of many nutrients are finite, partly because it promotes human health, both by reducing disease transmission and by increasing Agricultural production. An untapped resource such as sewage sludge that is rich in nutrients and humus-forming materials could replace parts of the commercial fertilizers used in Sweden today. owever, sewage sludge contains undesirable substances such as heavy metals and drug residues, and pathogenic microorganisms.

Chemical substances are spread diffusely and uncontrollably as a result of the increased consumption of goods. REVAQ is a certification system that works to reduce the flow of hazardous substances to Swedish treatment plants and to create a sustainable nutrient recycling. According to REVAQ, the yearly accumulating rate of metals should not exceed 0.2% in soil per year. Silver and bismuth are two metals that do not fulfill this requirement. The silver concentration in sludge decreased during the last decade, however the last few years, it has leveled off.

Bringing back the nutrients from the city to the country is becoming increasingly important, as the world?s stores of nutrients get more exhausted. Sewage sludge is an unapplied resource, rich in plant nutrients, which could replace parts of the commercial fertilizers used in Sweden today. Sewage sludge may contain large amounts of pathogens, such as Campylobacter and Salmonella, why use can cause severe spreading of infections, both to animals and humans. These pathogens have the ability to survive for a long time in soil and water and therefore some sort of treatment of the sludge is recommended before use.

The purpose of this study has been to make an inventory of combustible waste in the region around Norrköping and to investigate the composition of domestic waste, industrial waste and two different types of sludge. The work was performed at the request of Norrköping Miljö & Energi, due to the planning of an incineration plant. The inventory of the waste was performed through inspection of environmental impact reports, from waste disposal plants in a number of local government areas in the region. The quantities were also compared to another inventory, carried out in a different way. The characterisation of the wastes was compared to the fuel specification determined by Norrköping Miljö & Energi.

In the next few years the pollutants loading at the Slottshagen wastewater treatment plant in Norrköping is going to increase due to the reason that one of the waste water treatment plant in the district will be shut down.A survey of how these different pollutants are divided in the treatment process is therefore necessary for mapping out both the loading and treatment efficiency.This can be achieved by studying mass balances.Mass balances were carried out for the water treatment process for the mechanical/chemical,biological and the chemical stage.The mass balance studies show that the treatment efficiency was very good. However in order for the mass balances to be quite consistent and to ensure complete coverage of results and conclusions i.e. more reliable results, sludge samples should be collected and taken at different times per day and the influent rate should be investigated.In this work mass balance has been set up in order to illustrate and identify the factors that should be considered and taken care of for further estimation using mass balances in the future. Furthermore this survey results in a calculating model for the mass balances at thisplant.This calculating model is used as a tool to facilitate future calculations for mass balances for the water treatment process at Slottshagens wastewater treatment plant.Moreover it provides a basis for carrying out further estimation of the treatment process in the near future.In this project the importance of some operating parameters was also taken in consideration.This calculating model can be modified so that calculations of such operating parameters i.e. sludge loading and sludge age can be easily performed.Such information can be used further to evaluate different operation alternative in order to ensure an optimal use of the treatment process with in the plant..

Hallsta paper mill uses large amounts of water during the pulp and paper making processes. The wastewater is treated in two separate activated sludge processes referred to as BIO 1 and BIO 2. The main aim is to reduce the organic substance content, measured as COD (Chemical Oxygen Demand) before the wastewater is released into the environment. Since the effluent produced is very rich in organic substances, the addition of nitrogen (N) and phosphorus (P) are necessary to give satisfactory treatment results. Problems with sludge bulking sometimes occur which lead to increased levels of suspended matter and nutrients in the effluent.

Chemical stabilization of mine waste from Ljusnarsbergsfältet with sewage sludge and calcium carbonate residuesMine waste from Ljusnarsbergsfältet in Kopparberg, Sweden, is considered to constitute a great risk for human health and the surrounding environment. Some of the waste rock consists of sulphide minerals. When sulphide minerals come into contact with dissolvedoxygen and precipitation, oxidation may occur resulting in acid mine drainage (AMD) and the release of heavy metals. The purpose of this study has been to characterise the waste material and try to chemically stabilize the waste rock with a mixture of sewage sludge and calcium carbonate. The drawback of using organic matter is the risk that dissolved organic matter can act as complexing agents for heavy metals and in this way increase their mobility.

AbstractThe dependency of fossil fuels in the transport sector causes large emissions of carbondioxide. This problem can we reduce by using vehicle gas from digested solid manure and leftover of pasture. I have studied the potential for this in the county of Västernorrland. Thepurpose is to investigate how much vehicle gas that can be extracted.One central, large scale digestion and upgrade plant should be placed in Härnösand. Theamount of pasture and manure that is economical and practical available is enough to produce2,7-3,5 millions Nm3 vehicle gas.