Flowcytometric technology has been widely used for measurement of the absolute numbers of T-lymphocytes subsets in Human Immunodeficiency virus (HIV), defining the disease state, monitoring antiviral treatment, and identifying any risk for opportunistic infections. A manual preparing of the samples is required. More recently an automated and enclosed blood cell counting, Celldyn Sapphire has been introduced. In this study the performance of the Flow cytometer EPICS XL as a reference method for analysis of CD3+, CD4+ and CD8 T-lymphocytes was evaluated with blood from 40 individual?s samples.

This student report is about canine infectious skin diseases. It is primarily directed at veterinary assistans. The report includes a description of the skin?s structure and immunologic defences. The four types of allergic reactions are described. Different diagnostic techniques are also accounted for. The skin diseases included in the report are: fleas, lice, Sarcoptes scabiei, ringworm, Cheyletiella and leishmaniosis.

The main interest of this study was to investigate whether there is hyperglycemia or hypoglycemia in cows suffering from abomasal displacement and to evaluate the effect of transport on blood glucose. Thirty-six cows with left- or right-sided abomasal displacement were included in the study together with 17 cows with other diseases for comparison. Fifteen cows with abomasal displacement and 7 cows with other diseases were sampled before transport. One third of the cows with abomasal displacement had hyperglycemia when the transport effects were corrected for. Separating this group in left- and right-sided abomasal displacement we found significantly higher blood glucose and insulin levels among cows with right-sided abomasal displacement. An explanation for this difference may be a strong stress response secondary to the right-sided dislocation.

The purpose of the present study was to study if the systemic blood pressure in cats significant increases due to stress associated with a visit to a veterinarian clinic. We also wanted to investigate the clinical utility of two different methods for indirect blood pressure measurements in cats by assessing how easy they were to use and how reliable measurements they provided. The methods that were used were oscillometric (Memo Diagnostic) and ultrasound-doppler technique (Minidop). The blood pressure was measured in both clinical environment and home environment. Fifty cats were used in the study, from which measurements were impossible to obtain in one cat.

The aim of the work was to investigate the possibility to combine information from PPG and bio-acoustic technology to extract information that is related to the blood pressure. The measurements have been carried out with several different configurations. First the relation between arterial sounds and the PPG-signal was studied. After those measurements with both PPG and the bio- acoustic technique was concluded on people in different positions and after riding a bike. The goal was to vary the blood pressure.

SAMMAMFATTNINGBakgrund: Reaktiv hyperemi definieras som ett övergående ökat blodflöde över det normala efter en tids ischemi. Det kan registreras med laserdopplerteknik. Att registrera reaktiv hyperemi är ett sätt att värdera mikrocirkulationen. Det finns många flödesvariabler att studera varav tid till maxflöde efter ocklusion är en. Det har gjorts studier som undersöker om det finns ett samband mellan reaktiv hyperemi och fysisk aktivitet/syreupptagningsförmåga.

Two post milkning teat dips (PMTD´s) were compared in an automatic milking system at Kungsängen Research Farm, owned by SLU (Swedish University of Agriculture) in Uppsala. In the experiment an evaluation system was used where teat condition regarding skin and teat ends was graded according to a scale where 1 was the best score and 5 the worst. The evaluation period was almost six months in total devided into four minor experimental periods. The technician scoring the teats did not know which PMTD that was currently used. Statistical analysis showed a significant difference between the two PMTD´s regarding teat skin condition, but not regarding teat ends.

Native spider silk is an attractive lightweight natural polymeric material with a unique combination of strength, extendibility and flexibility. Synthetic, recombinant spider silk is an attractive biomaterial with several possible fields of application. When a foreign material is introduced to blood, this can for example cause unwanted coagulation and complement activation. The aim of this study was to investigate if coatings of heparin or albumin could improve the blood compatibility of films of recombinant spider silk and to develop an initial method to do so. Hypothetically, objects like pacemakers, stents and dental implants could be covered with spider silk films of high blood compatibility to reduce potentially harmful foreign body reactions caused by the immune defense. Increased blood compatibility would likely lead to improvements for both the individual patients and the society thanks to reduced load on healthcare institutions. Our objective was to assess the blood compatibility of both functionalized and non-functionalized recombinant spider silk by evaluating coagulation and activation of the complement system of the immune defense. Coagulation was evaluated visually on glass slides and complement activation was analyzed by measuring the concentration of the complement system mediator C3a in blood samples that had been exposed to the different materials. This study introduces a non-toxic and easy performed method of improving the blood compatibility of recombinant spider silk films by adding a heparin coating. Heparin coated surfaces had statistically significantly higher blood compatibility than uncoated surfaces. The effects of albumin coating of films were less clear compared to the results of heparin coated films. Complement activation could not be evaluated because of high activation in all samples prior contact with the materials that were included in the study..

Platelet aggregation is a major and common problem in blood samples from cats. This greatly affects the accuracy of counting of the platelets. Because of the difficulties in counting feline platelets, results for patients and reliable reference values for cat platelets are currently so inaccurate as to be almost useless in diagnosis of thrombocytopenia. In this study we used an effective method to prevent platelet aggregation. Blood was collected in CTAD test tubes and prostaglandin E1 was added to the blood sample.

 This report deals with thecollaborative project between FagrellProdukt Utveckling AB and studentsfrom the Faculty of Science andTechnology at Uppsala University. Theoverall objective of the project wasin addition to developing students'abilities, to help Fagrell withverification and development ofFagrells prototype, Fundamental flowreactor, for flow chemistry. Theuniqueness of Fagrells flow reactor isthat it should be simple andaffordable compared to competing flowreactors in the market.The verification was done bylaboratory and simulation sessions.The verification shows that Fagrellsidea on how to construct a flowreactor is very good.The development included bothtechnology and business. The result ofdevelopments can be helpful for thecommercialization of Fagrell flowreactor..

Background: Earlier research describes patients with blood-bourne pathogens experiences from the healthcare system. Aim: The aim of this study was to describe how registered nurses experience taking care of patients with blood-bourne pathogens. Method: This study had a descriptive design with a qualitative method. Eight interviews where performed and analyzed with manifest content analyze. Three categories ?Comfort in their profession?, ?Support from the organization?and ?Fear of being contaminated?, and five subcategories ?Support from hygiene guidelines?, ? Thoughtfulness?, ?The own responsibility?, ?Feelings of fear and concern?and ?To meet preconceptions?, emerged.

Myxomatous mitral valve disease is a common disease in older dogs of small- to medium-size breeds. The mitral valve, and less commonly the tricuspid valve may be affected which causes them to leak blood from the ventricle to the atrium of the heart during systole. With increasing severity of mitral valve leakage the forward stroke volume ejected into the aorta decreases, which may lead to an increase in blood pressure in the pulmonary vein resulting in pulmonary edema. A decrease in cardiac output may lead to a decrease in blood pressure, but the body may respond in order to normalize the blood pressure. Franzén showed in 2007 that the systolic blood pressure was increased in dogs with severe mitral insufficiency.

Aim: To examine the reaction of extreme preterm infants with regarding to body temperature and transepidermal water loss during skin-to-skin care according to Kangaroo Mother Care (KMC) during the infant?s first week of life. Method: This was a descriptive quantitatively designed study, which was a pilot study within the framework of a larger project. Nine children, with a median gestational age of 24.91 weeks, were examined by measuring body temperature (axilla and skin temperature) as well as transepidermal water loss before, during and after KMC. Results: The study showed that skin temperature tended to rise during KMC, especially for those children who were nursed with KMC for more than 60 minutes.

Detta  examensarbete  undersöker  skillnaden  i  tre  olika  nybörjargruppers  upplevda flow  då  de  spelat  Magicka  (2011)  med  motståndarantal  som  den  enda  skiljande variabeln.   Undersökningen   baseras   bland   annat   på   Csikszentmihalyis   (1990) flowteori och Caillois (2001) teori om att tävlande slutar vara roligt då det  urartar i kaos.  Syftet  med  examensarbetet  har  varit  att  hitta  när  mest  flow  uppstår  då nybörjare på Magicka (2011) spelar mot  en viss motståndartyp, samt om  detta flow bryts  när  för  många  motståndare  introduceras.  Undersökningen  har  visat  att  nio motståndare har resulterat i betydligt mer flow än tre, medan skillnaden mellan nio motståndare  och  18  har  varit  obetydlig.  Examensarbetet  har  dock  inte  lyckats kvantifiera  var  flow  övergår  till  kaos  med  den  testgrupp  och  motståndartyp  som använts.  Framtida  forskning  skulle  exempelvis  kunna  undersöka  på  vilket  sätt  ett varierande  motståndarantal  skulle  påverka  flow  för  en  mer  erfaren  testgrupp  som spelar mot andra motståndartyper..

The horse is a homeothermic animal and to keep a constant body temperature in winter season is important. Acclimatisation is to provide means for a good thermal insulation consisting of skin, hair, and an outer air layer. The skin covers the horses body and it is composed of three main layers where the top layer of the epidermis consists of dead keratinized cells that are constantly being sheed. The dermis is composed of collagen connective tissue that give the skin its strength. The bottom layer with subcutaneous fat provides a thermal insulation and can be used as an energy reserve in cold weather.