This EEF-project is a part of a larger project at SLU, Division of Reproduction, Dept of Clinical Science, with the intention of developing and evaluating new methods for the assessment of stallion semen quality. Over the last 10 years there has been an increase in the use of artificial insemination with cooled semen in Swedish horse breeding. At the same time, foaling rates have decreased. This has lead to a need for new and better methods of assessing the quality of stallion semen. This EEF-project was based on an objective method to calculate motility, with QualispermTM, in fresh and cooled stallion semen, and after selection. Today sperm motility evaluation is mainly done subjectively by visual assessment with a light microscope, and the correlation between these motility results and fertility have varied.

Today, equine breeding is based on performance and conformation, resulting in a vast variation in fertility among different horses, with noticeable economic losses as a consequence. The horse breeding industry is therefore in need of methods that diagnose the spermatozoa concerning their quality and provide the best sperm for AI. The commonly used selection methods for sperm today are: centrifugation, where extender is added, the sample is centrifuged and then resuspended; swim-up self-migration; adherence separation; and density gradient centrifugation. However, none of these methods are in used routinely in practice before inseminating mares with fresh or cooled semen. The aim of this study was to evaluate a new selection method, Single Layer Centrifugation (SLC), and its effect on sperm viability. The viability was analyzed with regard to membrane integrity and membrane stability of ejaculated spermatozoa.

The aim of the thesis is twofold: the first aim is to identify a number of reproductive logics that are used to construct Swedish regulation of embryo donation for reproductive use as either ethical or unethical in the governmental ? and medical discussions ? and to examine how these logics are interconnected with notions of gender and parenthood. The second aim is to identify a number of tensions that arise in the application of the governmental ethical logic on the embryo and embryo donation and discuss how these tensions can be solved with a feminist material approach. I argue that the terminology used to distinguish between genetic, biological, social and legal parenthood in my material is insufficient for understanding the value of the embryo outside its pre-human status. I therefore argue for an embryonic feminist bioethics that is attentive to the embryos post-human status, and for a reformulation of the parenthood terminology in line with what I call ?reproductive ties?..

In the early 1860´s, the author and opinion former Fredrika Bremer made a large donation of books to Högre lärarinneseminariet, which is the first governmental institute for higher education of women in Sweden. The aim of this thesis is to describe the collection of donated books as well as to extract information regarding Bremer's international network of contacts, her views on women's education and her relation to Högre lärarinneseminariet. Furthermore, the aim is to highlight the unique relationship between Fredrika Bremer's private collection of the books and the library of Högre lärarinneseminariet from a gender perspective. A handwritten list of the 321 titles constitutin the donation has been preserved in the seminar archive and three quarters of the books have been re-discovered in a bookcase at Årsta castle. The donation is characterized by its double origin as stamps and personal dedications remain as permanent traces in the books.

En operationssjuksköterska kan under sin yrkesverksamma tid förväntas att ansvara vid en operation där patienten konstaterats hjärndöd och det beslutats att organdonation ska genomföras. Syftet med denna studie var att beskriva operationssjuksköterskors erfarenheter vid organdonation när donatorn är hjärndöd. Studien har en kvalitativ ansats, där forskarna strävar efter en helhetsförståelse av det studerade intresseområdet. Datainsamling utfördes via ostrukturerade intervjuer med öppna frågor. Sju intervjuer genomfördes med operationssjuksköterskor som medverkat vid organdonation när donatorn var hjärndöd.

EVA, Equine Viral Arteritis, was diagnosed for the first time in 1953 in USA. The disease gained international interest in 1984, when there were several outbreaks of abortion at Thoroughbred studs in USA. EAV, Equine Arteritis Virus, is an RNA virus in the Arteriviridae family. There is only one serotype of EAV but several strains and the strains have different virulence. EAV is transmitted in two different ways: by aerosols, or by venereal transmission.

In this trial we used QualispermTM as an objective method of measuring stallion semen. Samples of semen were analyzed before and after centrifugation. Motility and concentration were statistically analyzed. During the trial all the objective mesaurments were compared to a subjective method. The advantages of QualispermTM compared to older CASA- systems is it´s ability of measuring a greater amount of spermatozoa while it also grades them into different subpopulations. These subpopulations have different qualities that decides their capacity of fertilization. The results showed that this objective method might be used practically. Although, there are still some disadvantages, such as high costs and the necessarity of accurat training of the handler. If it´s possible to prove further relevant results that also are statistically correct, this could prove the usefulness of QualispermTM as an objective measurment method in the daily work at gestuts and stallion stations, thus giving the staff a reliable instrument in assisting the work for an improved horse breeding in Sweden, improving both in quality and economically..

At SLU, Divison of Reproduction, Dept of Clinical Science, there is an ongoing project on the development and evaluation of new methods for the assessment of stallion semen quality. This EEF-study, which is a part of that larger project, has focused on the effects on motility and fertility of stallion semen of two different extenders and selection. Both an objective method and a subjective method for analysis have been used and compared in the study. The increase in the use of artificial insemination with cooled semen in Swedish horse breeding over the last decade is thought to play a part in declining foaling rates (Kareskoski et al, 2006, Kuisma et al, 2006). This calls for better evaluation methods of semen quality since cooling and transportation put higher demands on semen quality in order to maintain fertility (Aurich, 2005).

AbstractThe purpose of this bachelor thesis is to describe the use of artificial insemination (AI) in sheep and discuss the possibilities for AI in Sweden. Male and female fertility, heat detection, semen handling and insemination techniques in sheep are described. Advantages and disadvantages of AI are discussed. In order to achieve genetic progress it is important with well planned and implemented breeding programs. The Swedish Sheep Recording Scheme and computer software from Elitlamm are the basis for sheep breeding in Sweden.

Uganda is one country with fastest growing populations in the world and with more than 25% of the population living in poverty. There is a rapid rural urban migration with increasing demands for food for low income earners especially in these areas. One way to mitigate food insecurity is to increase milk and meat production efficiency, and thus reproductive performance of cows is crucial for good production. A well-documented strategy for improving dairy cow productivity through faster genetic improvement is to breed dairy cows using proven semen via artificial insemination (AI). AI has been used in Uganda for over 60 years but only less than 10 %, a small population of the country?s herd has been bred that way. The aims were to study knowledge, attitude and practices factors influencing cow fertility results on dairy farms around Kampala using AI and to propose ways of promoting increased use of AI in Uganda.

The use of artificial insemination (A.I.) as an alternative to normal mating in dog breeding in Sweden are controlled by regulations from the Swedish Board of Agriculture and by ethical rules assigned by the Swedish Kennel Club. Only veterinarians, who have a specific certificate and are specially trained and educated, are allowed to perform inseminations in dogs. Each artificial insemination must be documented and reported to the Swedish Kennel Club (SKK) which, on the behalf of the Swedish Board of Agriculture, yearly presents statistics concerning the use of A.I. in Sweden. The objective of this study was to illustrate the use of artificial insemination in dog breeding in Sweden for the last decade, using the yearly reports from the Kennel Club during the period of 1995 to 2004.

IntroduktionGenom klinisk erfarenhet är det känt att bemötandet av närstående är en central fråga för hela donationsprocessen, men närstående till organdonatorer framstår samtidigt som en eftersatt grupp när det gäller uppföljning. Att ta fram vetenskaplig kunskap om närståendes erfarenheter av donationsprocessen är nödvändigt för förståelsen och bemötandet av dessa personer.SyfteSyftet med denna studie var att kartlägga närståendes erfarenheter av donationsprocessen såväl i det akuta skedet som efter att själva donationen har genomförts. MetodStudien har genomförts i form av en enkät. Enkäten skickades ut till 74 närstående som hade haft en anhörig som avlidit och blivit en organ- och vävnadsdonator. Enkäten besvarades av 54 % (n=40).

Following the wake of the AIDS-catastrophe in the beginning of the eighties, homosexual men or 'men who have sex with men' (MSM) have been barred from donating blood throughout the planet. In this thesis I look into the different discourses at play in the context of creating the legal framework for blood donation in Sweden. Genealogically tracing the emergence of the category of MSM and mapping how the category is brought to use in contemporary negotiations on blood safety, I scrutinize how scientific truth is established within blood transfusion practice. I argue that the rationality of risk group exclusion is contingent on economical grounds, and that the foucauldian concept of biopolitics could productively be used to understand this rationality. In particular, the concept of the 'biopolitics of blood' can be employed in order to understand the reluctance from the involved actors to acknowledge the heteronormative consequences of the legal framework of contemporary blood donation..

Bakgrund Idag väntar många Svenska medborgare på ett eller flera nya organ. Organ doneras av personer som har avlidit i total hjärninfarkt. Intensivvårdssjuksköterskorna vårdar både organdonator och närstående till organdonator. Mötet mellan närstående och intensivvårdssjuksköterskan är ett mycket speciellt möte då de närstående är i en sorg- och krissituation. Syftet är att beskriva intensivvårdssjuksköterskors upplevelser av mötet med närstående till patienter som avlidit akut och som blivit organdonatorer.

Bakgrund: Bröstmjölk har positiv inverkan på nyfödda barns hälsa, särskilt för förtidigt föddaoch sjuka barn. Donation av bröstmjölk ger fler nyfödda barn som behöver vård på sjukhusmöjligheten att få bröstmjölk som näring under deras första tid i livet.Syfte: Att undersöka kvinnors upplevelser av att donera bröstmjölk samt att undersöka hurkvinnor som donerat bröstmjölk tror att antalet donatorer kan ökas.Metod: En kvalitativ enkätstudie. 23 enkäter skickades ut till kvinnor som donerat bröstmjölktill Akademiska Barnsjukhuset i Uppsala under perioden 130831 ? 140901.Resultat: Kvinnorna hade övervägande positiva upplevelser av att donera bröstmjölk. Denfrämsta anledningen till valet av donation var viljan att hjälpa behövande barn.