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184 Uppsatser om Reporter gene assay - Sida 11 av 13

Begomovirus susceptibility in wild Nicaraguan tomato populations

Tomato production is a common and important agricultural activity in Nicaragua. During the last decades tomato producers have suffered great yield losses due to begomoviruses (family Geminiviridae). The genome of begomoviruses generally consists of two circular ssDNA components, DNA-A and DNA-B. Tomato yellow leaf curl virus (TYLCV) is an example of a begomovirus with only one component. An approach to manage the virus epidemics has been to breed a cultivar with begomovirus resistance genes, which have been transferred from wild tomato.

Genome editing with the CRISPR Cas9 system

Rapporten ingår i ett FoU- projekt för Högskolan Dalarna, där målet är att ta fram en konstruktion utan ångspärr som ska klara dagens mått på lufttäthet och fuktkrav.Syftet med denna rapport är att utreda hur fukt påverkar en byggnad medmassivträstomme och olika isoleringsmaterial utan ångspärr. Mineralull och träfiberisolering jämförs mot varandra för att se hur dessa påverkarfuktbelastningen i en väggkonstruktion. Testobjektet är lokaliserat i Dalarna, inget fukttillskott har funnits inomhus i byggnaden.För att genomföra detta arbete har tre stycken olika metoder används. Ensimulering, verkliga uppmätta värden och en provtagning. Fuktsimuleringen genomfördes med hjälp av programmet WUFI, uppmätta värden i form av relativ fuktighet och temperatur har samlats in kontinuerligt under två års tid från väggkonstruktionen via mätsensorer.

Varför uppförs så få bostadsrätter med passivhusstandard i Sverige? : En fallstudie mellan ett passivhus- och en konventionell fastighet

Abstract Background: In 1997 the Kyoto Protocol was signed, it?s an international agreement which is aimed to reduce greenhouse gas emissions. Sweden's goal is to reduce energy using in buildings with 20 percent by 2020 and 50 percent by 2050. This is a comparison with levels from 1995 (Glad, 2006). For Sweden to be able to reach its goals, they need to construct more low-energy buildings (Åhman, Wahlström & Lundblad, 2010).

Vad är nyttan med en ökad specialisering?

Syftet med detta examensarbete är att undersöka vilken nytta den nya gymnasieutredningens förslag om ökad specialisering och förbättrad anställningsbarhet kan ha för elever inom Hantverksprogrammet ?Textil & Mode . Gällande frågan om bedömningen av en sådan nytta, har fokus i undersökningen i första hand inriktats på vilka krav och önskemål om kompetens som finns inom hantverks- och konfektionsbranschen idag men även i ett framtidsperspektiv. Undersökningen bygger på en kvalitativ forskningsansats med utgångspunkt från ett antal halvstrukturerade intervjuer med olika verksamheter inom hantverk och konfektion. De gene-rella resultaten visar på att kraven för anställningsbarhet inom den mer kvalificerade kärn-verksamheten, ser ut att ligga bortom det grundläggande kunskapsuppdrag som yrkesutbild-ningen inom gymnasiet kan avses ha.

Amyloid beta inducerad klyvning av NG2 medierad via LRP-1 receptorn

Bakgrund: Deposition av fibrillär amyloid beta 1-42 (A?) i hjärnan är ett välkänt kännetecken för den neurodegenerativa sjukdomen Alzheimer?s (AD). Dessa ansamlingar påverkar pericyter, en celltyp involverad i blodkärlsfunktion och upprätthållande av blodhjärnbarriären (BBB). Pericyter uttrycker både receptorn low density lipoprotein receptor related protein 1 (LRP-1) till vilken A?1-42 binder, och proteoglykanet NG2.

Med kriget som arbetsplats : Pressfotografers upplevelser av arbetsvillkoren i krig och konflikter

Syftet med denna studie var att undersöka svenska pressfotografers upplevelser av arbetsvillkoren i krig och konflikter, för att därigenom nå en ökad förståelse för den problematik som medier ställs inför i bevakningen i krig. Studien är baserad på kvalitativa intervjuer med tre svenska pressfotografer, samtliga med flerårig erfarenhet av arbete i oroshärdar. Resultatet visade att arbetssituationen omgärdas av en rad komplexa och samverkande villkor på olika nivåer. Förkunskaper och erfarenhet framhölls som viktiga faktorer i det dagliga arbetet, liksom betydelsen av goda relationer till kollegor och lokala kontakter. Arbetet sker vanligtvis i team tillsammans med en reporter, vilket av samtliga fotografer beskrevs som positivt, inte minst säkerhetsmässigt.

Evaluation of transgenic lines of field pepperweed (Lepidium campestre L.)

The worldwide industrial demand of energy, often in form of fossil fuel, is continuously growing and at the same time these resources are coming to an end (Hamamre, 2013). The interest and focus in renewable sources are then more important than ever and plants are suggested to be a good alternative for production of raw material for industrial purposes (Nilsson et al., 1998). But the world is also facing an increasing world population which also increase the nutritional needs, plants therefore need to be suitable for both industrial and nutritional purposes. To achieve this, common plants need to be improved but it also need new potential crops to be developed and introduced (Carlsson et al., 2009). Modern technology like gene engineering is an important tool to achieve these challenges (Carlsson et al., 2011). This work focused on analyzes and evaluation of different transgenic lines of field pepperweed (Lepidium campestre L.).

Cross-sectional study of the prevalence of Babesia bigemina in Uganda : wildlife-livestock interface at and around LMNP

Ticks and the diseases they transmit are of major importance throughout the world. In Uganda, cattle are the most important livestock from an economic point of view. Livestock keepers fear bi-directional transmission of tick-borne pathogens between their livestock and wild animals. This cross-sectional study was conducted to establish and compare the sero-prevalence of the tick-borne pathogen Babesia bigemina among randomly selected Ankole Long-horned cattle and European crossbred cattle on 30 farms in Kiruhura district, in two sub-counties near Lake Mburo National Park in South-western Uganda. Half of the farms were situated in close proximity to the park and thereby housed cattle with more frequent wildlife-livestock interface (Sanga), whereas the other half had less frequent contact (Kikatsi).

Precisionsnutrition vs. Standardiserade Kostr?d Finns det en effekt med precisionsnutrition j?mf?rt med standardiserade kostr?d f?r viktnedg?ng? - En Systematisk ?versiktsartikel

Syfte: Syftet med denna systematiska ?versiktsartikel ?r att unders?ka det vetenskapliga underlaget om precisionsnutrition har en st?rre effekt p? viktnedg?ng j?mf?rt med standardiserade kostr?d vid ?vervikt och obesitas hos vuxna personer. Metod: En litteraturs?kning utf?rdes och inkluderade randomiserade kontrollerade humanstudier som unders?kte viktf?r?ndringar med hj?lp av precisionsnutrition. Inklusionskriterierna omfattade vuxna ?ver 18 ?r med ett BMI ?ver 25. Datainsamlingen utf?rdes genom s?kningar p? PubMed och Scopus med specificerade s?kord och filter.

Etik i tanke och praktik : - en kvalitativ studie i hur studenttidningsredaktioner resonerar kring etik.

Title: Ethics in thought and practice ? a qualitative study in how editorial staff at student magazines reason about ethics. (Etik i tanke och praktik ? en kvalitativ studie i hur studenttidningsredaktioner resonerar kring etik)Number of pages: 30 (37 including enclosures)Author: Bianca GidebackTutor: Peder Hård af SegerstadCourse: Media and Communication studies CPeriod: Spring 2010University: Division of Media and Communication, Department of Information Science,Uppsala University.Purpose/Aim: The purpose of this study is to explore how students that work at student magazines reason about ethics of journalism and how they transform it into practice. By identifying what circumstances in the context of the student magazine that can affect the work this study aim to see if and how this can affect how they conform to ethics of journalism in practical situations.

Odour-mediated behaviour in codling moth, Cydia pomonella : do fermentation odours affect the attraction and oviposition behaviour in codling moth?

The codling moth, Cydia pomonella is a common pest in apple orchards and can be found worldwide. Feeding by codling moth larvae can cause a substantial decrease in apple yield. Control methods comprise for example the use of pesticides, viruses and pheromones. As a sustainable control method, pheromones have been successfully applied to disrupt moth mating behaviour. Other behaviours like foraging, search for mating sites or host finding could be potential targets for additional odour-mediated behavioural manipulation but need further investigations of the underlying odour stimuli and behavioural plasticity. Many insects are attracted to the smell of fermenting fruit and associated microorganisms.

Vilken information får hundägare i samband med kastrering av sin hanhund?

BACKGROUND Until 1988, it was forbidden to neuter male dogs in Sweden without a veterinary medical cause, so there is clearly no long tradition of this procedure in healthy dogs. According to the website of The Swedish Kennel Club (SKK), February 11, 2011, there has been an increase of castrated male dogs in Sweden. They see this increase as worrying, especially in cases of the less common breeds, where it is most important to maintain the gene pool. The SKK expresses concern that castration is used as a tool to influence the personality of dogs, instead of training and education of the dog. They write that castration should be preceded by a discussion initiated by the veterinarian, where the pros and cons of castration are weighed against each other.

Bärarskap av meticillinresistent Staphylococcus pseudintermedius (MRSP) hos svenska hundar :

During the last years, meticillin resistant Staphylococcus pseudintermedius (MRSP) have caused an increasing number of infections in Swedish dogs. MRSP is resistant against a majority of the antimicrobials available for dogs in Sweden, making it difficult to treat the infection. Our knowledge of the bacterium is limited, making it hard to handle infected dogs in a proper way. The aim of this study was to investigate for how long time dogs, who have an infection caused by MRSP, become carriers of the bacterium. Other factors like treatment with antimicrobials, the correlation between clinical symptoms and carriage, common diagnoses and institutionalization where also looked upon.

Genetiska studier på fågelinfluensa :

In the spring of 2006 we had our first outbreak of avian influenza of highly pathogenic H5N1 (HPAI) among wild birds in Sweden. This disease have impact on poultry industries world wide. Sporadically the virus infects other species than birds and we now know it can even cause fatal infection of humans. There are not yet any confirmed cases of transmission of the disease between humans though a suspected case in an Indonesian family is under investigation. If a mutation occurs that changes the pathogenicity and host affinity to humans we are at a risk of a new pandemia, perhaps like the Spanish flu in 1918-1920.

Echinococcus multilocularis in wild boar : aiming at an alternative surveillance method

This study was the first part of a larger project investigating a new surveillance method for Echinococcus multilocularis. In this study we examined livers from wild boar (Sus scrofa), looking for lesions caused by E. multilocularis. E. multilocularis is a small tapeworm which has an indirect life cycle.

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