Sökresultat:
9069 Uppsatser om Real time measurement - Sida 10 av 605
TaqMan® Sample-to-SNP Kit? : evaluation of kit for low-cost and fast preparing of DNA-samples before genotype analysis
Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .
detection and quantification of almond (Prunus dulcis) in food with ELISA
Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.
Konstruktion av småsäckstömmare
Scania CV is one of the world's largest truck manufacturer. It is not only the directproduction of vehicles and methods that are important for Scania but also the servicemarket handling spare parts, repairs and workshops. The department Vehicle ServiceInformation construct standard times through work measurement for particularworks in the workshops. The software application STAM is used in the servicemarket to create and store standard times which then are exported to theworkshops' own systems.The purpose of this master thesis is to analyse and evaluate the internal workmeasurement system that includes standard times and the software STAM. Also,based on external analysis and benchmarking of other systems suggest appropriatefuture development within the area for Scania Vehicle Service Information.In order to perform the investigation, interviews and studies have been doneinternally at the departments that are involved in work measurement as well asexternally at Scania workshops.
Den uppkopplade enkätundersökningen : En studie av informationsvisualiseringen i Mentimeter
A new phenomenon on the Internet is the online audience response. Mentimeter is a web-based tool where you can create online polls. The users can then cast a vote and the result is shown in real time. We want to test the information visualization in Mentimeter and we base our hypothesis, that a better graph design is possible, on the work of Edward Tufte and Stephen Few. Edward Tufte is an emeritus at Yale University, where he held courses in statistical evidence and information design and has created theories about how visual information should be designed.
En studie av skillnader mellan monotisk och dikotisk 40 Hz Auditory Steady-State Response
ObjectiveThe objective of this study was to investigate differences between monotich and dichotic 40 Hz Auditory Steady-State Response (ASSR) with Interacoustics Eclipse.DesignThe aspects studied are test duration, relation be¬tween ASSR and behavioural thresholds (BT), reproducibility and correlation between rejects during Brainstem Response Audiometry (BRA) and differences between ASSR and BT. Monotic and dichotic ASSR-thresholds for eight normal hearing subjects (.
Automatisk rengöring av produktionsbana
Scania CV is one of the world's largest truck manufacturer. It is not only the directproduction of vehicles and methods that are important for Scania but also the servicemarket handling spare parts, repairs and workshops. The department Vehicle ServiceInformation construct standard times through work measurement for particularworks in the workshops. The software application STAM is used in the servicemarket to create and store standard times which then are exported to theworkshops' own systems.The purpose of this master thesis is to analyse and evaluate the internal workmeasurement system that includes standard times and the software STAM. Also,based on external analysis and benchmarking of other systems suggest appropriatefuture development within the area for Scania Vehicle Service Information.In order to perform the investigation, interviews and studies have been doneinternally at the departments that are involved in work measurement as well asexternally at Scania workshops.
Konstruktion och beräkningar av skogsmaskinshytt
Scania CV is one of the world's largest truck manufacturer. It is not only the directproduction of vehicles and methods that are important for Scania but also the servicemarket handling spare parts, repairs and workshops. The department Vehicle ServiceInformation construct standard times through work measurement for particularworks in the workshops. The software application STAM is used in the servicemarket to create and store standard times which then are exported to theworkshops' own systems.The purpose of this master thesis is to analyse and evaluate the internal workmeasurement system that includes standard times and the software STAM. Also,based on external analysis and benchmarking of other systems suggest appropriatefuture development within the area for Scania Vehicle Service Information.In order to perform the investigation, interviews and studies have been doneinternally at the departments that are involved in work measurement as well asexternally at Scania workshops.
Utveckling av ett provtagningssystem för alkohol och aldehyder i fordonsemissioner.
A system for sampling of alcohol and aldehyde from diluted exhaust gases from vehicles powered by ethanol has been developed and tested. The major part of the work has been research of earlier work in the field and the construction of the sampling system.The part of the system constructed for the measurement of alcohol has been shown to work, however, more test should be performed. The part of the system constructed for the measurement of aldehyde was tested parallel to a commercially available system, based on the same principle of measurement, and has been shown to work well.Determination of the concentration of gaseous ethanol using a FID has shown the since earlier known fact that a FID is not able to measure a correct concentration of ethanol. Furthermore it has been shown that high concentrations of gaseous ethanol can not be kept in a bag more than a few minutes without a substantial decrease of the measured concentration..
Demonstrationsrigg för mätning av vätskors egenskaper
Scania CV is one of the world's largest truck manufacturer. It is not only the directproduction of vehicles and methods that are important for Scania but also the servicemarket handling spare parts, repairs and workshops. The department Vehicle ServiceInformation construct standard times through work measurement for particularworks in the workshops. The software application STAM is used in the servicemarket to create and store standard times which then are exported to theworkshops' own systems.The purpose of this master thesis is to analyse and evaluate the internal workmeasurement system that includes standard times and the software STAM. Also,based on external analysis and benchmarking of other systems suggest appropriatefuture development within the area for Scania Vehicle Service Information.In order to perform the investigation, interviews and studies have been doneinternally at the departments that are involved in work measurement as well asexternally at Scania workshops.
Musikterapi som redskap inom barnsjukvården : forskningsöversikt avseende smärta och rädsla
The aim is to describe the factors that influence nurses' assessment and measurement of pain in patients. Literature study based on 13 reviewed articles, searched in the Cinahl. Qualitative and quantitative articles were analyzed. Six themes were identified that can be seen as the factors that influence nurses' assessment and measurement. The themes were pain education, nurses´ experience in the profession, nurses´ level of knowledge about pain relief, documentation about pain, nurse´s perspective on the patient, and pain measurement.
Optimization of immunoassay parameters in multiplex in the high throughput protein detection technique Proximity Extension Assay
The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.
Det svenska konsumtionsbeteendet-En ekonometrisk analys av den permanenta inkomsthypotesen
Abstract Titel: Det svenska konsumtionsbeteendet ? En ekonometrisk analys av den permanenta inkomsthypotesenTopic/course: NEKK01, Bachelor thesisWriters: Ida Hedlund, Anna Persdotter Supervisor: Fredrik NG Andersson Keywords: Permanent income hypothesis, Milton Friedman, Consumption smoothing, Sweden Purpose: The purpose of this thesis is to examine whether the permanent income hypothesis can be applied on the consumption pattern of Sweden during the time period 1950-2006. Method: To do the observation an econometric regression analysis has been made. The variables tested are consumption, real GDP, stock- and real estate market prices. In the model three different time periods have been investigated to facilitate the origin of different patterns.
Prissättning av fastighetsförmedling: en fallstudie av tre fastighetsmäklare
The aim of this thesis is to get an idea of how service companies set their prices. The method used was a case study approach, which was conducted at three real estate companies. The study showed that when setting their prices, the main aspect is their own costs. However, we have found that several other aspects play a big role when setting prices on services in real estate business such as individual customer behaviour and market competition. We have also found that the real estate companies charge very similar prices.
Reala optioner : Konsten att tydliggöra värden i tidiga venture investeringar
Analysts at Venture Capital firms of today experience troubles when they try to explicate the intrinsic value, which they see in their investments. The traditional valuation models do not account the value of flexibility and analysts have to Through studies at a Venture Capital firm in Sweden, I have analyzed if Real Option analysis could help them to explicate the intrinsic value that they see.With the help from a decision tree, analysts can identify the hidden option values embedded in the investment. I find that real options can be a helpful tool for Venture Capital companiesto clarify the intrinsic values that they see in their investments. Real option analysis is however not a competitor to traditional value methods, it is rather a compliment..
När ett vallöfte blir verklighet : Analys av DN:s rapportering om fastighetsskatten innan och efter valet 2006
Did the mass media report in a different way about the real estate tax before the Swedish national election 2006 than they did after the election? If there were differences, what could be the cause?This study aims to answer these questions by using a quantitative analysis of articles about the real estate tax published in the Dagens Nyheter (DN), the biggest morning daily. The summer before the election, the ?Alliance?, (the non-socialist coalition, launched an election promise to abolish the real estate tax. Instead they planned to introduce a low community charge.The Alliance won the election and formed a government to implement their election promise. The analysis was made on all the published articles in DN that covered the real estate tax issue. In total there were 43 such articles. These where all published between the launch of the campaign promise until the electionday and a month before the proposal was launched 19/9 2007, until the proposal was implemented in 2008. The study results in three interesting conclusions.