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9046 Uppsatser om Real time PCR SYBR-green - Sida 10 av 604

Pilotstudie för att utvärdera effekten av L-Mesitran : honungsbaserad sårsalva : vid behandling av yt-pyodermi hos hund.

Green manuring is a way of creating an organic fertilizer by growing a crop that is mowed down. Besides the fertilizing effect this method has been shown to have a positive influ-ence on soil organic matter as well as weed flora, and green manure is an important me-thod of weed control in organic farming in parts of Sweden. However, the release of nu-trients from the green manure varies and can therefore be difficult to control. Risks for losses of nutrients are also present. Although the area of organic farmland is increasing in Sweden, the use of green manure is decreasing.This report contains a literature study which compiles research on the functions of green manure in a cropping system and also how the crop can be used if harvested, for example to produce biogas.

Östra Kvillebäcken En gröna stadsdelen? En fallstudie över Östra Kvillebäcken i Göteborg

The study is a case study about the green urban district, East Kvillebäcken, located in Gothenburg, Hisingen. East Kvillebäcken is the first urban district in Sweden with ambition to build sustainable according to the certification system Miljöbyggnad, which make it a pioneer project. The purpose is to investigate the upcoming and the meaning of the green urban district in East Kvillebäcken and show how public employees think the urban district can affect social, ecologic and economic aspects in the area. Dialogue interviews were done with four public employees, one estate agent and two residents. Local plans and other authority documents were used to get a deeper understanding for the case study.

Utveckling av programvara till ett inbyggt styrsystem

This thesis considers the development of the software for an, newly developed, embedded real-time control system, based on a Periferal Interface Controller (PIC). The control system is developed to be used for several different applications. In this thesis work, software for two different applications are developed. The first task is a time controlled system, while the other task is implementation of aPI-/PID-controller. A user interface is also developed and implemented.The time controlled system is an automatic horse feeder, which is used for feeding horses at certain predefined points in time.

Förslag till bebyggelse och park i kvarteret Seminariet i Uppsala

In the urban planning debate of today, two strategies for city development stand out as each other's opposites, urban sprawl and smart growth. This thesis concerns a development project of the latter kind and aims to give an alternative proposal to new buildings and a park in a Swedish city block. The term sprawl is used for new developments on virgin soil on the outskirts of cities leading to a city spreading, while smart growth stand for increased city density through development on re-used land within the city boundaries. The latter are often seen as more sustainable in many levels and are also shown to have positive effects on a number of city functions. At the same time smart growth has negative consequences on other aspects of the city such as public open spaces.

TaqMan® Sample-to-SNP Kit? : evaluation of kit for low-cost and fast preparing of DNA-samples before genotype analysis

 Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .

Hur påverkar förtätningen stadens grönstruktur? :

Urban density is not new, but rather one side of the continous processes of change underway in the city. During the urbanizing boom of the industrialization, when the cities grew bigger, the first spaces to be built on were vacant and planted areas in the interior of the district. The gardens that once were there were replaced by yardhouses. The idea of urban density is that a dense city means less distance between residential areas and workplaces, resulting in fewer shipments and travelers, which ultimately leads to lower energy consumption and lower emissions. Urban density may lead to shorter distances, but not necessarily. In the wake of urban density the greenstructures of the city is shattered. The possibilities for green areas to contribute to the health of the city and its inhabitants is dramatically reduced.

Gröngödslingens roll i odlingssystemet : en jämförelse mellan Mälarregionen och Östergötland

Green manuring is a way of creating an organic fertilizer by growing a crop that is mowed down. Besides the fertilizing effect this method has been shown to have a positive influ-ence on soil organic matter as well as weed flora, and green manure is an important me-thod of weed control in organic farming in parts of Sweden. However, the release of nu-trients from the green manure varies and can therefore be difficult to control. Risks for losses of nutrients are also present. Although the area of organic farmland is increasing in Sweden, the use of green manure is decreasing.This report contains a literature study which compiles research on the functions of green manure in a cropping system and also how the crop can be used if harvested, for example to produce biogas.

detection and quantification of almond (Prunus dulcis) in food with ELISA

Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.

Den uppkopplade enkätundersökningen : En studie av informationsvisualiseringen i Mentimeter

A new phenomenon on the Internet is the online audience response. Mentimeter is a web-based tool where you can create online polls. The users can then cast a vote and the result is shown in real time. We want to test the information visualization in Mentimeter and we base our hypothesis, that a better graph design is possible, on the work of Edward Tufte and Stephen Few. Edward Tufte is an emeritus at Yale University, where he held courses in statistical evidence and information design and has created theories about how visual information should be designed.

Handla hos oss - så klimatkompenserar vi! : Hur stora välkända företag påverkar sina konsumenters inställning mot deras varumärke med hjälp av grön marknadsföring och CSR.

Title: Shop at us ? and we?ll carbon offset! ? How large and well-known companies affect consumers feelings towards their brand by using green marketing and CSR.Authors: Emilie Jäfvert & Carolin RuthbergAdvisor: Christine TidåsenLevel: Bachelor thesis in Marketing, (15 Swedish credits), Spring 2011Keywords: Green marketing, CSR and branding.Question: How do large and well known companies affect their customer?s view towards their brand by using green marketing and CSR?Purpose: The purpose of this thesis is to investigate how high consumer?s value companies who works with green marketing and CSR, and also to find out how it affects the company brand.Method: We have chosen to do a qualitative research with a deductive approach. We have done five open interviews with companies and ten open interviews with consumers to conduct our research.Theoretical framework: Our theoretical framework includes four different areas; CSR- Corporate Social Responsibility, green marketing, brands and consumers. These sub-chapters include theories that will help the reader get a better understanding of the areas.Empirical studies: We have gathered empirical data by multiple open interviews. Two of the five interviews with the companies were face-to-face, two via email and one over the phone.

Optimization of immunoassay parameters in multiplex in the high throughput protein detection technique Proximity Extension Assay

The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

Klimatanpassning med grönstrukturplanering : Med exempel från Malmö stad

Climate change is expected to lead to increased rainfall and higher temperatures, among other things. This will have a major impact on our cities. Floods can lead to property damage as well as damages to human and animal health. Higher temperatures will increase the frequency of heat-related health risks and premature deaths. The consequences of climate change will be particularly large in our cities because of the city's morphology with a high amount of paved surfaces and buildings that affect the natural processes in areas such as drainage and absorption of solar radiation.

Det svenska konsumtionsbeteendet-En ekonometrisk analys av den permanenta inkomsthypotesen

Abstract Titel: Det svenska konsumtionsbeteendet ? En ekonometrisk analys av den permanenta inkomsthypotesenTopic/course: NEKK01, Bachelor thesisWriters: Ida Hedlund, Anna Persdotter Supervisor: Fredrik NG Andersson Keywords: Permanent income hypothesis, Milton Friedman, Consumption smoothing, Sweden Purpose: The purpose of this thesis is to examine whether the permanent income hypothesis can be applied on the consumption pattern of Sweden during the time period 1950-2006. Method: To do the observation an econometric regression analysis has been made. The variables tested are consumption, real GDP, stock- and real estate market prices. In the model three different time periods have been investigated to facilitate the origin of different patterns.

Gröna broar : ekologiska, sociala och estetiska tillgångar

The visions of the United Nations concerning sustainable development and conservation of biodiversity can only be realized if the human population succeeds in creating coexistence between human and wildlife. Landscape architects are able to influence on how landscapes are to be maintained and developed. The negative impact of infrastructure on wildlife has become a critical problem in Sweden and other parts of Europe. Wildlife crossings has been developed as a solution for the problems of road barriers in the landscape. The crossings are made to give animals a better opportunity to cross roads.

En hållbar framtid med Gröna Byggnader.

På Sveriges fastighetsmarknad har man de senaste åren kunnat urskilja ett allt högre fokus påmiljön från dess aktörer. Miljödebatten i Sverige har snabbt fått fäste och allt fler företag förstårvärdet av att fokusera på sådana frågor.Att utvecklingen fortsätter att gå framåt är viktigt då fastigheter och dess tillhörande byggnaderstår för nästan en tredjedel av världens utsläpp av växthusgaser och bara byggandet och driftenstår för cirka fyrtio procent av den globala förbrukningen av råvaror och energi. Prioritering avmiljöfrågor blir således viktiga vid fastighetsförvaltande och nyproduktion.Tidigare forskning visar att det finns en mängd incitament för att bygga och förvalta grönt. Ävenhyresgästerna har incitament för att hyra gröna lokaler. Vidare visar internationella studier att detinte alltid krävs stora åtgärder för att uppnå bra resultat vad gäller fastigheters energiförbrukningoch hållbarhet över tid.

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