The cattle production in the world has been questioned in Sweden due to its negative impacts on the environment. The use of imported soya bean meal as a protein feed for Swedish cattle results in the cutting of rainforest and an extensive use of pesticides in the countries were the soya beans are produced. As a consequence, the environmental impacts caused by cattle production increases. A production of protein feeds in Sweden would decrease the transportations and the cutting of rainforest. For organic beef producers the possibility to produce home-grown protein feeds is important since organic protein feeds can be difficult to obtain in another way.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

This project investigated how elevation and vegetation type influences variation in plant litter phenolic content and protein precipitation capacity among and within common plant species for two different vegetation types, heath and meadow, in a subarctic ecosystem in the Abisko region of northern Sweden. As nutrient availability generally decreases with increasing elevation as a result of decreasing temperature, I hypothesised that phenolic content would increase with elevation and be higher on the heath than the meadow. To test this, the total phenolic content and protein precipitation capacity was estimated in leaf litter from 13 species in both heath and meadow vegetation across an elevational gradient ranging from 500 to 1000 meters above sea level (m.a.s.l.) in the study region. The results showed that elevation and vegetation type both had a strong impact on both variables. Total phenolic concentrations decreased with elevation for the meadow, and were greater for the heath than the meadow.

An excessive input of nutrients to lakes and other water bodies has created a problem with eutrophication in Sweden. Untreated, or partially treated, domestic sewage is a major source for discharge of phosphorus (P), which is the nutrient most frequently responsible for eutrophication of most fresh waters and the Baltic Sea. The waste water can be cleaned by filter materials, which have a high P-retention ability and which after saturation may be used as fertilizers. Four potentially suitable filter materials were tested in batch- and column experiments in this study. In the batch experiments, the following materials were shaken with waste water in time series ranging from 5 seconds to 60 minutes: coarse (1-4 mm) and fine (0-2 mm) Polonite® (heated bedrock from Poland); Filtralite® (light expanded clay aggregates with limestone added before burning); water cooled blast furnace slag (BF-slag) and BF-slag mixed with 10% burned limestone.

The objective of the present study was to evaluate distillers? dried grains with solubles (DDGS) as a protein source to rainbow trout (Oncorhynchus mykiss), by studying digestibility and growth measurements. One control diet, totally lacking DDGS, and three test diets were made containing 10 % (low), 31% (medium) and 51 % (high) DDGS respectively on dry matter (DM) basis. The control diet and the test diets were made to be as nutritionally equal as possible. Wheat bran was used to balance the fiber content of the diets.

Mechanical scaling is one of the most complex operations of all in the mining process. The operators are using both hearing, sight and feel to read the rock and to know where to scale and where to leave. They say they go on gut feeling in great extent, something that comes with experience. The scaling machine is placed into the edge of unsecured and newly blasted rock to hammer off the blocks that has become loose after the blast, but not enough to fall down. It goes without sayingthat it is a risky job.

Användningen av biomarkörer inom medicin är ett viktigt verktyg för att ställa diagnos och fatta terapetiska beslut. Surfaktant protein D (SP-D) har visat sig vara en lovande biomarkör för perifera lungsjukdomar på humansidan och har därför även börjat studeras inom veterinärmedicinen. Surfaktant protein D är ett relativt lungspecifikt protein och produceras främst i perifera delar av lungorna, f.f.a av claraceller i bronkioler och typ II pneumocyter i alveoler. Det fungerar som ett akutfasprotein med liknande egenskaper som kollektiner och är därför viktigt för medfödda immunförsvaret. Förutom detta har det också visat sig vara grundläggande för normala lunghomeostasen genom att utgöra en beståndsdel i surfaktant. Mekanismerna bakom dess funktion i lungan är dock inte helt klarlagda. Eftersom SP-D är så basalt för lungan påverkas det ofta kraftigt vid perifera lungskador.

In Sweden more than 400 000 private households have not yet sufficient wastewater purification. These effluent is considered as an increasing problem and many onsite purification methods have been studied. In this investigation, the method of reactive bed filters have been tested by column and batch experiments. Five different kinds of filter materials with reactive surfaces have been studied concerning their capacity to absorb ammonium and phosphorus from wastewater. The materials that were examined are Filtra N, wollastonite Filtra P, blast furnace slag and Polonite®.

Det kommer ständigt nya studier med olika hälsoråd angående kosten och fysiskaktivitet om hur vi ska undvika fetma, högt blodtryck samt hjärt- och kärlsjukdomar.Intresset av proteinets effekt på uppbyggnad av muskelmassa vid fysisk aktivitet harökat. Detta har medfört ett stort urval av olika drycker med proteintillskott påmarknaden, främst ur animaliskt ursprung.Syftet med detta examensarbete var att ta fram en vegetabilisk proteinberikadhavredryck som förväntas hjälpa kroppen vid återhämtning. Arbetet innebar att utvecklaett recept baserat på de Nordiska Näringsrekommendationer 2004 (NNR) med fokus påprotein, kolhydrater och fett. Delmålet var även att utesluta allergener. Dryckenbaserades på ett medelvärde från de fysiskt aktiva och äldre människors näringsbehov,detta för att bredda den tilltänkta dryckens målgrupp.Fyra smaksatta drycker framställdes med olika energiinnehåll utifrån två olikahavredrycker, en naturell och en kalciumberikad.

Horses are grazers with a digestive system that is adapted for a high fiber diet. Forage is a fiber rich feed that includes grass and legumes. A deficiency of forage and an excess of grains may cause gut disturbances and stereotypic behavior. The aim of this literature study was to summarize information on the horse?s capacity to consume forage-only diets with focus on the horse?s need of energy and protein.

Sahlgrenska Academy at University of GothenburgDepartment of Internal Medicine and Clinical Nutrition3AbstractTitle: How does isoflavones in combination with soy protein impact cholesterol levels in hypercholesterolemic postmenopausal women relative to milk protein?Author: Emma Edberg och Emma NilssonSupervisor: Lena HulthénExaminer: Anna WinkvistProgramme: Dietician stydy programme,180/240 hpType of paper: Examination paper, 15 hpDate: 2014-05-26Background: Hypercholesterolemia is a major risk factor for cardiovascular disease and the number one cause of death among women in Western countries. Declining levels of estrogen at menopause could be a contribution factor. Isoflavones is a vegetable substance, with structural and functional similarities to the human estrogen. This is a potential reason for possible cholesterol-lowering effects.

The coproduct, dried distillers grains + solubles (DDGS) from ethanol production based on wheat contains much rumen degradable protein and fiber with low digestibility. To compliment this quality in an effective way a grass silage low in protein and much digestible fiber is needed. The aim of the study was to investigate the effects of adjusting the crude-protein concentration and fiber quality of the silage when feeding DDGS and of varying the inclusion level of DDGS, when feeding the adjusted grass silage, on intake, milk yield and composition, faecal traits and feed efficiency. Four different total mixed rations were compared. The normal grass-clover silage had a crude protein content of 17,8 % and an NDF content of 46,2 % of dry matter. The adapted grass silage had a crude protein content of 14,6 % and an NDF content of 52,9 % of dry matter. The diets were formulated to have similar nutritional content except for A + 4 which had a higher content of rumen degradable crude protein and a lower concentration of NDF.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.