The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

Two species of bees in the genus Apis, real honey bees, has long been of interest for man. These two are the European honey bee, Apis mellifera, and the Asian honey bee, Apis cerana. In Vietnam, beekeeping is of great importance, both with A.cerana and A.mellifera. The aim of this project was to investigate if the introduction of the European honey bee in Asia has affected the Asian honey bee, and whether different pathogens from A.mellifera have been transferred to A.cerana. Totally 40 samples, 20 from every species, were analysed for 8 different viruses.

To save energy it?s popular to build houses with low energy loss. In these houses the walls are keeping a lower temperature in the external side which leads to a higher relative humidity. In addition more material, such as wood studs, are used which means that there is more moisture to dry. More moisture in combination with higher relative humidity may pose a higher risk of microbial growth.

Background: As a part of a national health control program, Statens VeterinärmedicinskaAnstalt performs diagnostics to screen flocks for certain pathogens causing high mortality,morbidity and/or serious economical losses. There are several viruses in the programincluding IBDV (infectious bursal disease virus), IBV (infectious bronchitis virus) and NDV(Newcastle disease virus). Method: 96 serum samples were collected from different poultryflocks in Sweden and analyzed by ELISA, which are currently used in the health controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well as antibodies of avian reovirus. In the context of this study the ELISAs run inroutine diagnostics as well as a REO ELISA were used as the standard for comparison.Result: The antibody concentration in serum from vaccinated chickens was high while theantibody concentration level in serum from not vaccinated chickens was low.

Background: As a part of a national health control program, Statens VeterinärmedicinskaAnstalt performs diagnostics to screen flocks for certain pathogens causing high mortality,morbidity and/or serious economical losses. There are several viruses in the programincluding IBDV (infectious bursal disease virus), IBV (infectious bronchitis virus) and NDV(Newcastle disease virus). Method: 96 serum samples were collected from different poultryflocks in Sweden and analyzed by ELISA, which are currently used in the health controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well as antibodies of avian reovirus. In the context of this study the ELISAs run inroutine diagnostics as well as a REO ELISA were used as the standard for comparison.Result: The antibody concentration in serum from vaccinated chickens was high while theantibody concentration level in serum from not vaccinated chickens was low.

Concentration of protein in bovine milk is one of the most significant milk quality parameters, to a large extent determining the price for milk to the producer. Mastitis is a common disease among dairy cows, negatively affecting not only milk yield but also milk protein composition. Milk from mastitic cows tends to have lower cheese yield, negatively affected processability properties and sensory quality, due to changed protein quality and composition. Poorer milk protein quality would have an economically negative impact on dairy industry. Only few studies have investigated the short term effects of mastitis on milk protein composition.

Många byggnader är idag fuktskadade. Fuktskador uppstår på så väl nya som gamla byggnader. Idag provtrycker man hus för att få ett värde på dess täthet, men många hus som klarar täthetskraven drabbas ändå av fuktskador. Detta beror på att otätheterna är lokala på en eller flera delar av klimatskalet bortsett från otätheter vid anslutningar.  En studie har gjorts på två väggtyper; en standardvägg och en passivhusvägg.

The purpose of this study was to examine whether urea could be an option as a nitrogen source for young cattle, the study was limited to risks related to feeding, growth and feed efficiency. Microbes in ruminants have the ability to convert nitrogen to protein, this has been highlighted by beef producers as they are in search of cheap protein feed resources. Urea can be a worthy source of nitrogen for growing young animals, because of its high nitrogen content and the low price. Studies have shown that the replacement of a portion of the total protein content by urea in diets with low content of crude protein, like corn silage, may improve animal growth and feed efficiency. Replacement of all the true protein with urea can result in reduced feed intake and microbial growth.

This paper aims to, through a literature review, examine how to best design and adapt calf huts to meet the calf's physiological requirements and reduce the infection pressure for enteric and respiratory diseases. It illuminates how the calf relates to different temperatures and climate, which temperature control mechanisms it uses and its ability to perform these regulations, as well as providing a summary of the past decades´ research on the calf huts housing system. These two theory chapters, along with a concluding discussion, provide a better understanding of how to, especially in temperate climates, design the most suitable calf hut, which cater to the calf's both thermal comfort and physical health. The literature suggests that the calf's own temperature control works well to counteract the temperature outside the desired range, and that one of the most important factors rather is ventilation of a calf hut, which reduces moisture and pathogens. The conclusion of this paper is mainly that there is not an obvious way to place and construct a calf hutch and that most design principles contribute to both positive and negative effects simultaneously.

In Sweden, there are limits to how much leachable substances waste must contain in order to be deposited as hazardous waste. Fly ash from waste incineration often end up over the limit, mainly due to the chloride content. Fly ash is therefore often deposited abroad. The purpose of this study was to investigate the possibility to handle fly ash locally. To clarify how the law is applied, environmental reports and permit documents from ten waste sites with permission to deposit fly ash were studied.

Background: As a part of a national health control program, Statens VeterinärmedicinskaAnstalt performs diagnostics to screen flocks for certain pathogens causing high mortality,morbidity and/or serious economical losses. There are several viruses in the programincluding IBDV (infectious bursal disease virus), IBV (infectious bronchitis virus) and NDV(Newcastle disease virus). Method: 96 serum samples were collected from different poultryflocks in Sweden and analyzed by ELISA, which are currently used in the health controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well as antibodies of avian reovirus. In the context of this study the ELISAs run inroutine diagnostics as well as a REO ELISA were used as the standard for comparison.Result: The antibody concentration in serum from vaccinated chickens was high while theantibody concentration level in serum from not vaccinated chickens was low.

Beställningsfrekvensen för detektion av Trichomonas vaginalis samt Mycoplasma genitalium i Jönköpings län är låg jämfört med den för Chlamydia trachomatis och Neisseria gonorrhoeae.  Både T. vaginalis och M. genitalium har associerats med infektion av humant papillomvirus (HPV) samt kan bland annat orsaka salpingit med infertilitet som potentiell komplikation. Patogenerna har även beskrivits öka risken för transmission av HIV. Syftet med studien var att detektera T.

The probiotic bacteria Lactobacillus reuteri DSM17938 was evaluated as a starter culture in cheese production, with the intention to produce a new interesting product within the functional food market. Growth and acidification rate in milk subjected to different heat treatments, as well as supplementation with growth promoting factors or support cultures was measured during controlled fermentations. The results showed that the weak proteolytic activity of L. reuteri could be compensated for by supplementation with 1% casamino acids or 5% MRS broth. Furthermore, a decrease in growth and acidification rate could be seen when using yoghurt culture or Lactobacillus delbrueckii as adjunct cultures.

Biogas is increasingly used for fuel in for example vehicles and it´s produced in a biogas processconsisting of the steps of pretreatment, digestion and gas cleaning. The pretreatment is a method usedto increase the gas production and / or destroy pathogens. The digestion is the stage when anaerobicmicroorganisms convert bio-mass of a substrate to a raw gas containing about 65% of methane. Thegas cleaning is used to increase the methane content to about 97% so the gas can be used for motors invehicles. The biogas part of the Ekeby sewage plant in Eskilstuna is using multiple substrates.

The water level of Lake Mälaren has been regulated since the 1940?s. A recentinvestigation suggests that the current regulation needs to be adjusted in order toprevent a decline in water level, salt water from infiltrating from Lake Saltsjön,and risk of floodings. In addition to these preventions, the new regulation couldpotentially effect the natural environment and agriculture practices in the lowlands that surround the lake. The new regulation proposal calls for an increase invariations in Mälaren?s water level during seasons, while the annual mean waterlevel will remain the same.