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8524 Uppsatser om Method Validation - Sida 3 av 569
Validation of realtime-PCR of Fusarium avenaceum for detection in wheat
Mould is a common contamination in cereals. The growth of mould can stimulate mycotoxins production andsome of which at critical concentrations cause health problems in humans and animals. Fusarium is one of thefungus species that has been found in crops and can cause major problems for farmers such as reduced harvestand economic losses. A group of Fusarium species, Fusarium avenaceum, Fusarium poae and Fusariumtricinctum express a mycotoxin, enniatin. The limited information available today about enniatin-forming fungiis that they grow out on fields of wheat in colder climates.
Utveckling av en LC-MS-metod för analys av gamma-hydroxibutyrat, gamma-butyrolakton, 1,4-butandiol, amfetamin och metadon
In this project a LC-MS-method for the analysis of gamma-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, amphetamine and methadone was developed.Initially, the efficiency of the ionisation of the analytes was evaluated with respect to the ionisation technique (ESI, APCI and APPI) and the composition of the mobile phase. In the next step a number of different columns was tested in order to find the one with the greatest potential for separation of the substances in question. Using the selected column, the separation was optimised by means of experimental design and the software The Unscrambler 7.8. The parameters studied were the flow rate, the column temperature and the mobile phase composition. The response variables were the resolution between the target compounds and the retention time of the last eluting compound.These experiments showed that, in order to obtain the best ionisation, the mobile phase should consist of 5 mM formic acid in water and acetonitrile.
Verktyg för lönsamhetsberäkningar vid bränslekonvertering av spetslastpannor från olja till pellets
This report summarizes the development of a calculation program estimating the profitability of converting a peak-load oil-fired boiler to pellets. To convert an oil-fired boiler to pellets a new fuel handling system must be designed. Changes also have to be made to some other components. Since there are many different techniques for transportation, storage and treatment of the fuel only the most interesting techniques from Swecos experience and interest were included in the study.The capacity of the different parts in the system was dimensioned based on the power of the boiler after conversion. To estimate prices, installed power and dimensions manufacturers and distributers were contacted.
Uppnår sjuksköterskestudenter lärandemålen?
Objective: To see if nursing students believe that they have acquired the knowledge that the Higher Education Ordinance demand and if there is any difference between the theory learned at the University and the clinical reality in which students receive their practical training. Method: The method is quantitative and is divided into two parts, one in which the authors describe how the questionnaire was developed and how validity was strengthened. In the second part, the authors describe how the data analysis was made and how reliability of the result was strengthened. Result: The result shows that the students felt that they have achieved most of the objectives of learning demanded by the Higher Education Ordinance. But some objectives are perceived as not at all or only partly achieved.
Självskadande kvinnors upplevelse av bemötande i vården
The purpose of the study was to describe how women with deliberate self-harm behaviour experience they have been treated when conducting health care. The study has a qualitative and descriptive approach and data was collected with snowball sampling. Data was analysed with content analysis and ended up in three theme ?Feeling of violation of integrity and autonomy?, ?health care staff don´t have the courage to step in and take responsibility? and ? Personal confirmation and validation of feelings?. The results showed that many women with deliberate self-harm behavior feel that they have been badly treated when they conducting health care.
Effekten av traktkil på det distala benets rörelsemönster hos travhäst :
Changing of the hoof angle is a common method for the treatment of many lameness-related conditions (Moyer 1980; Stashak 2002). Various studies in the literature have indicated different results of the effect of changing the hoof angle on the movement of the distal limb. Our study investigated the effect of raising the heel of the forelimbs by 10,7 mm on the movement of the distal limb. Horses trotted on a treadmill at several predetermined speeds. The kinematics of the distal limb was determined and variables including the extension of the fetlock joint were determined.
Kreditklassning av aktiebolag i Sverige, en logistisk regression
Predicting corporate failure is an increasingly important topic in the world of economics today. This paper, with the help of the credit ranking company Syna AB, aims to investigate a few different statistic strategies to do just that. This task is accomplished by using a data material of 250 000 Swedish companies divided into two subsamples. The first sample is used to develop the model and the second as a validation sample. The model developing sample holds 160 000 companies divided into five different subgroups based on size and age.
Metodfokus på Affekt; Hur känns det?
Evidence-based psychotherapeutic methods compete with each other, while meta-analysis have shown that variability due to different methods related to outcome is remarkably low. In this qualitative study six former patients were interviewed about experiences of method and technique in Affect-focused therapy, with a slight overweight towards unsatisfactory experiences. Responses were analysed and categorised in emergent themes. Methodological focus on affect showed to be a much appreciated, as well as insufficient, element. Alongside positive experiences or summaries of therapy, methodological frames were felt to be at times restrictive, even invalidating, as far as not allowing focus on what was felt to be the more predominant need.
Volume and taper equations for Sitka spruce (Picea sitchensis (Bong.) Carr.), Norway spruce (Picea abies (L.) Karst.) and White spruce (Picea glauca (Moench) Voss) in Iceland
The aim of this study was to evaluate different types of volume and taper equations that can be used to predict single-tree stem volume and stem diameter at any given height along the tree stem for plantation grown Sitka spruce (Picea sitchensis (Bong.) Carr.), Norway spruce (Picea abies (L.) Karst) and White spruce (Picea glauca (Mounch) Voss) in Iceland. A number of published tree volume equations were tested and modified to predict the total stem volumes over bark but three logarithmic equations were taken for more in-depth analysis. Three taper equations were tested. Two variable-exponent equations (Kozak 1997, Kozak 2004) and one exponential equation described by Biging (1984). Data from a total of 617 sample trees were used in this study, collected from stands in various parts of the country and present different types of stands growing in different soil types and cover most of the site conditions suitable for forestry in Iceland.
Bättre med Scrum? : En studie om den "nya" utvecklingsmodellen
The Thesis purpose is in the context of system development validate the Scrum Methodology. Projects concerning information technology are difficult to manage and tend to fail in quality, time or costs. Scrum presents a new view of the system development. Better cooperation between customers and developers, continuous follow-up on daily basis and extensive communication in the development team are all characteristics of Scrum. Scrum is described as a new paradigm by its founders but has been criticized by experts of system development too.
Skogsentreprenadföretagens lönsamhet :
During some time there has been a debate whether or not logging contractors? profitability can be considered poor. The contractors? situation is important for maintaining long term relationships and continuity in the forest industry. A thorough analytic picture is however missing.
Validation of rumination measurement equipment and the role of rumination in dairy cow time budgets
Automatic rumination measuring can be applied as a health indicator in dairy farming but is also highly desirable for research purposes. This study aimed to validate the technical functionality of the product RuminActTM, which employ acoustics to monitor rumination duration. The time budget of high yielding cows was studied and possible differences in rumination time between 13 rations were interpreted. Further the study examined what affects daily rumination and rumination efficiency. The validation was performed with direct observations that were compared to the automatically recorded rumination durations.
Initial evaluation of the Meritas Troponin I test for measurement of equine cardiac troponin I
Cardiac troponin I (cTnI) is considered a specific and a sensitive biomarker of cardiac disease, and due to a high inter-species sequence homology; human cTnI assays can often be used in veterinary medicine. The main aim of this study was to perform an initial evaluation of a human cTnI test, the Meritas Troponin I test, for measurement of equine plasma cTnI, in order to investigate whether the test could be a possible subject for a more comprehensive validation. The hypothesis was that the test could measure equine cTnI, as there is a good homology between equine cTnI and human cTnI.
The evaluation included a dilution parallelism, an intra-assay precision study and measurement of plasma cTnI healthy horses. In the dilution parallelism and the intra-assay precision study, equine plasma with previously established high cTnI concentration, according to another cTnI assay, was used. In addition, cTnI concentration was measured in plasma, collected from 19 horses, without signs of disease at physical and ECG examinations.
The obtained curve in the dilution parallelism was linear, indicating that the Meritas Troponin I test can be used to measure different concentrations of equine cTnI in plasma.
Optimization and validation of a triplex real-time PCR assay for thermotolerant Campylobacter species associated with foodborne disease
The genus Campylobacter is globally recognised as the leading bacterial cause of human foodborne gastroenteritis. Every year around 8000 Swedes are infected by Campylobacter. Most people are infected by thermotolerant Campylobacter species, commonly C. jejuni and C. coli.
?Flykten till Sverige var en flykt till frihet och inte till destruktivitet av min identitet? : En studie om invandrarakademiker på den svenska arbetsmarknaden
The aim of this essay is to examine how educated immigrants from former Yugoslavia are experiencing the Swedish labor market and if they feel that there are factors that complicate their ability to establish themselves in the Swedish labor market. We have also examined whether and if so, how their status has changed in connotation with the move to Sweden. The method we have used is a qualitative interview study where we asked questions from a semi-structured interview guide. We chose to make use of both previous research and a number of analytical tools for using these to interpret inequality and discrimination that exist in the Swedish labor against educated immigrants. The results presented four factors that our respondents perceive contribute to difficulty in establishing themselves in the Swedish labor market, these are: contacts, name, validation and trainee post.