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185 Uppsatser om Methicillin-resistant Staphylococcus aureus - Sida 12 av 13
Biological control of powdery mildew in greenhouse produced cucumber : an evaluation of two microbiological control agents
Powdery mildew is a serious problem in both field and greenhouse cucumber production and
can cause serious infection and radically reduced yields. Today, powdery mildew is the main
disease in greenhouse produced cucumber.
Disease control should preferably be both efficient and environmentally friendly, which is not
easy to achieve at all times. Alternatives to chemical control of powdery mildews are biological
control, physical control, non-fungicide control and cultivation of resistant or tolerant varieties.
Biological control can be described as the suppression of damaging activities inflicted
of a harmful organism by one or more other organisms, which are called antagonists or natural
enemies. In Sweden, there are currently no registered biological control products available
to control cucumber powdery mildew.
The aim of this study was to investigate the effect of two biological control products in order
to evaluate their ability to prevent or reduce powdery mildew infection to an acceptable level
in greenhouse produced cucumber. In the experiments, different application regimes were also
studied.
Effekt av olika kvävegödselmedel på utvecklingen av klumprotsjuka i salladskål (Brassica rapa ssp. pekinensis)
The price trend on rapeseed the latest years has led to an increasing interest to grow the crop among many farmers in Sweden. The price trend has resulted in more than a doubling of the oilseed area since the early 2000s. For many farmers it is an advantage to increase cultivation of rapeseed because of the high proportion of cereals in the crop rotation on many farms, but it is not just benefits. The risk of the fields being infected by clubroot increases within frequent rapeseed cultivation and it often leads to subtantial economic losses. Clubroot is caused by Plasmodiophora brassicae that is a soilborne pathogen in genus Protozoa and its resting spores can be found in the soil for up to 18 years.
Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden
The
focus
of
this
project
was
to
characterize
the
occurrence
of
ESBL-?producing
bacteria
in
two
irrigation
water
ponds
in
Southern
Sweden.
Samples
were
taken
from
two
ponds
nearby
each
other,
from
which
analyses
were
made
based
on
levels
of
community
and
individual
isolates.
Community
samples
were
used
for
attempts
for
characterization
of
resistance
patterns
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade.
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade..
Tillväxtreaktion hos skärmträd i högskärm av gran i Medelpad :
Different methods for shelterwood cutting have been used in differ-ent parts of Europe since the middle ages. The use of shelter wood cutting in Norway spruce (Picea abies (L.) Karst.) forests in Sweden met a revival in the early 1990s as an alternative for the dominating clear-cutting system when silvicultural costs increased dramatically for the latter. Shelterwood cutting systems involve some possible problems but also a lot of positive effects. Among the problems are increased risk of wind throw and damage of the remaining shelter trees caused by the cutting operation. Positive effects are for example a valuable growth in the remaining trees and increased biological di-versity.
This study is based on a total of 79 trees from 4 different treatments in a spruce shelterwood, cut in 1994, in the middle of Sweden (62,4º N).
Kartläggning av stallbyggnader och djurhållningssystem inom mjölkproduktion ur salmonellasynpunkt
Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.
Holmes och Watson ? Ett Queerläsningsäventyr : En undersökning av maskulinitet och sexualitet i Sir Arthur Conan Doyles The Memoirs of Sherlock Holmes
This thesis is a queer masculinity reading of Sir Arthur Conan Doyle?s short story collection The Memoirs of Sherlock Holmes (1894). The analysis focuses on the dissonances, tensions and queerness that reside within the text itself. This has been done from my problem statement: How is Sherlock Holmes and John Watson?s sexuality and masculinity portrayed within the boundary of the text? What is being said, what is hidden, and what is dealt with silently? To reveal these queer parts this analysis has been focused around five themes: the late Victorian male, the Woman, countertypes and decadence, the homosocial sphere and sexuality.
KRAV-grisar har fler ledanmärkningar än konventionellt uppfödda grisar.
Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.
The role and genetic control of non-coding RNAs in bacterial infection
Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.
Holdningskampen 1940 - 1942 och dess fortsättning : Med betoning på kyrkans roll som sammanhållande faktor under ockupationen av Norge
During the period of occupation of Norway between the years 1940-1945 the National Lutheran Church took the responsibility of supporting the Norweigans in their ideological battle against the Nazis. This was a period named Holdningskampen in Norwegian history. One person to remember was Eivind Berggrav, bishop in Oslo diocese. He was the one responsible for the theological document of importance Kirkens Grunn that became important as a document against the Nazis and the small percentage of Christians that related to the naziinspired-church. Berggrav was also one of the founders of Den Midlertidlige Kirkeledelsen, the organisation responsible for the non-nazified churches.
Hur skiljer sig ston åt i försvaret mot endometrit?
Kvarstående betäckningsinducerad endometrit är ett stort problem inom hästnäringen och så även andra typer av endometrit. Detta är den främsta orsaken till infertilitet hos ston. Vissa ston är nämligen oförmögna att klara av den inflammatoriska reaktion som normalt följer av betäckning (för att eliminera sperma och patogener) eller så drabbas de lättare av kroniskt infektiös endometrit, oavsett betäckning. Det finns otaliga förslag till förklaringar till varför de inte kan hålla uterus fri från inflammation såsom defekta myometriekontraktioner, stoets reproduktionshistoria eller anatomiska aspekter (t.ex. pneumovagina, otillräckliga mekaniska barriärer eller en horisontell uterus).
Metodik för resistenstest i renkavle : en studie om herbicidresistens; mekanismer, detektion och åtgärder
Herbicide resistance is the result of an evolutionary process where a population gradually changes from being susceptible to being resistant to an herbicide. The resistance mechanism is usually a change in the biochemistry of the weed, but in some cases changes in morphology or growth rhythm. The change is caused by random mutations. Two resistance mechanisms are found in black-grass; target site resistance and metabolic resistance.Black-grass is a tufty, winter annual grass weed, which is mainly found in milder climates. Black-grass is a big problem in Southern Europe.
Vindskador vid stickväg i 1:a och 2:a gallring i Boxholm, Östergötland : i stormen Pers fotspår
Wind damages next to strip roads in 1st and 2nd thinning in Boxholm, Östergötland
Anneli Fransson, SLU, inst f sydsvensk skogsvetenskap, Examensarbete no 108
Wind damage is something we are all familiar with since the storms Gudrun (2005) and Per (2007). Research about strip roads and wind damage is very limited. It is commonly known that the trees next to the strip roads more often fall whit heavy winds, and the purpose of this thesis was to verify this statement. Data was collected from first- and second thinnings at Boxholms Skogar AB, Östergötland. In first thinnings data was collected from both spruce and pine stands and second thinnings only data from spruce stands was collected.
Värdering av kvävet i organiska gödselmedel :
Within agriculture it is important to optimize the use of nitrogen, not only from an economic point of view but also to minimize damage to the environment. This can sometimes be difficult, especially within organic farming. Organic farmers are using increasing amounts of waste products from the food industry to cover their nitrogen needs. These different organic fertilizers release nitrogen at different rates and under specific soil conditions. Because of this, it would be beneficial to have a standardized method to measure the amount and rate of nitrogen mineralization from organic fertilizers.
This project contributes to this knowledge by investigating whether relatively simple and
repeatable laboratory methods can be used to estimate the nitrogen fertilizer value of organic fertilizers.
Enkätundersökning bland svenska veterinärer angående behandling av klinisk mastit hos mjölkkor
En webbaserad enkät kring behandling av klinisk mastit hos mjölkkor skickades till 741 svenska veterinärer som enligt Jordbruksverket rapporterat att man under 2012 behandlat fall av mastit. Studien gjordes med syftet att ta reda på hur klinisk mastit behandlas i Sverige 2013. Antalet svarande som inkluderades i studien var 267. Det innebar en svarsfrekvens på 36 %. Enkäten bestod delvis av allmänna frågor kring behandling samt tre fallfrågor.
Silver i luktfria kläder - en stinkande lösning
Genom ökad användning på träningskläder har antibakteriella behandlingar blivit allt vanligare för den allmänna konsumenten. Dessa plagg marknadsförs ofta som antibakte-riella eller luktfria, men det som inte framgår är vilka ämnen som används för att uppnå den luktfria effekten. Vanligt förekommande är de behandlingar som är baserade på oorganiska silversalter. Medan leverantörerna av dessa produkter argumenterar för, argumenterar många miljövetare och forskare på bakterier emot antibakteriella produk-ter då dessa kan ha en skadlig inverkan på vår miljö och hälsa. Rapporten syftar till att ge större kunskap kring miljö- och hälsopåverkan vid användandet av textiler behandlade med silversalter samt till att analysera konsumenters inställning till antibakteriella kläder samt deras användar- och tvättvanor för träningskläder.