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185 Uppsatser om Methicillin-resistant Staphylococcus aureus - Sida 12 av 13

Biological control of powdery mildew in greenhouse produced cucumber : an evaluation of two microbiological control agents

Powdery mildew is a serious problem in both field and greenhouse cucumber production and can cause serious infection and radically reduced yields. Today, powdery mildew is the main disease in greenhouse produced cucumber. Disease control should preferably be both efficient and environmentally friendly, which is not easy to achieve at all times. Alternatives to chemical control of powdery mildews are biological control, physical control, non-fungicide control and cultivation of resistant or tolerant varieties. Biological control can be described as the suppression of damaging activities inflicted of a harmful organism by one or more other organisms, which are called antagonists or natural enemies. In Sweden, there are currently no registered biological control products available to control cucumber powdery mildew. The aim of this study was to investigate the effect of two biological control products in order to evaluate their ability to prevent or reduce powdery mildew infection to an acceptable level in greenhouse produced cucumber. In the experiments, different application regimes were also studied.

Effekt av olika kvävegödselmedel på utvecklingen av klumprotsjuka i salladskål (Brassica rapa ssp. pekinensis)

The price trend on rapeseed the latest years has led to an increasing interest to grow the crop among many farmers in Sweden. The price trend has resulted in more than a doubling of the oilseed area since the early 2000s. For many farmers it is an advantage to increase cultivation of rapeseed because of the high proportion of cereals in the crop rotation on many farms, but it is not just benefits. The risk of the fields being infected by clubroot increases within frequent rapeseed cultivation and it often leads to subtantial economic losses. Clubroot is caused by Plasmodiophora brassicae that is a soilborne pathogen in genus Protozoa and its resting spores can be found in the soil for up to 18 years.

Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

Tillväxtreaktion hos skärmträd i högskärm av gran i Medelpad :

Different methods for shelterwood cutting have been used in differ-ent parts of Europe since the middle ages. The use of shelter wood cutting in Norway spruce (Picea abies (L.) Karst.) forests in Sweden met a revival in the early 1990s as an alternative for the dominating clear-cutting system when silvicultural costs increased dramatically for the latter. Shelterwood cutting systems involve some possible problems but also a lot of positive effects. Among the problems are increased risk of wind throw and damage of the remaining shelter trees caused by the cutting operation. Positive effects are for example a valuable growth in the remaining trees and increased biological di-versity. This study is based on a total of 79 trees from 4 different treatments in a spruce shelterwood, cut in 1994, in the middle of Sweden (62,4º N).

Kartläggning av stallbyggnader och djurhållningssystem inom mjölkproduktion ur salmonellasynpunkt

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Holmes och Watson ? Ett Queerläsningsäventyr : En undersökning av maskulinitet och sexualitet i Sir Arthur Conan Doyles The Memoirs of Sherlock Holmes

This thesis is a queer masculinity reading of Sir Arthur Conan Doyle?s short story collection The Memoirs of Sherlock Holmes (1894). The analysis focuses on the dissonances, tensions and queerness that reside within the text itself. This has been done from my problem statement: How is Sherlock Holmes and John Watson?s sexuality and masculinity portrayed within the boundary of the text? What is being said, what is hidden, and what is dealt with silently? To reveal these queer parts this analysis has been focused around five themes: the late Victorian male, the Woman, countertypes and decadence, the homosocial sphere and sexuality.

KRAV-grisar har fler ledanmärkningar än konventionellt uppfödda grisar.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

The role and genetic control of non-coding RNAs in bacterial infection

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Holdningskampen 1940 - 1942 och dess fortsättning : Med betoning på  kyrkans roll som sammanhållande faktor under ockupationen av Norge

During the period of occupation of Norway between the years 1940-1945 the National Lutheran Church took the responsibility of supporting the Norweigans in their ideological battle against the Nazis. This was a period named Holdningskampen in Norwegian history. One person to remember was Eivind Berggrav, bishop in Oslo diocese. He was the one responsible for the theological document of importance Kirkens Grunn that became important as a document against the Nazis and the small percentage of Christians that related to the naziinspired-church. Berggrav was also one of the founders of Den Midlertidlige Kirkeledelsen, the organisation responsible for the non-nazified churches.

Hur skiljer sig ston åt i försvaret mot endometrit?

Kvarstående betäckningsinducerad endometrit är ett stort problem inom hästnäringen och så även andra typer av endometrit. Detta är den främsta orsaken till infertilitet hos ston. Vissa ston är nämligen oförmögna att klara av den inflammatoriska reaktion som normalt följer av betäckning (för att eliminera sperma och patogener) eller så drabbas de lättare av kroniskt infektiös endometrit, oavsett betäckning. Det finns otaliga förslag till förklaringar till varför de inte kan hålla uterus fri från inflammation såsom defekta myometriekontraktioner, stoets reproduktionshistoria eller anatomiska aspekter (t.ex. pneumovagina, otillräckliga mekaniska barriärer eller en horisontell uterus).

Metodik för resistenstest i renkavle : en studie om herbicidresistens; mekanismer, detektion och åtgärder

Herbicide resistance is the result of an evolutionary process where a population gradually changes from being susceptible to being resistant to an herbicide. The resistance mechanism is usually a change in the biochemistry of the weed, but in some cases changes in morphology or growth rhythm. The change is caused by random mutations. Two resistance mechanisms are found in black-grass; target site resistance and metabolic resistance.Black-grass is a tufty, winter annual grass weed, which is mainly found in milder climates. Black-grass is a big problem in Southern Europe.

Vindskador vid stickväg i 1:a och 2:a gallring i Boxholm, Östergötland : i stormen Pers fotspår

Wind damages next to strip roads in 1st and 2nd thinning in Boxholm, Östergötland Anneli Fransson, SLU, inst f sydsvensk skogsvetenskap, Examensarbete no 108 Wind damage is something we are all familiar with since the storms Gudrun (2005) and Per (2007). Research about strip roads and wind damage is very limited. It is commonly known that the trees next to the strip roads more often fall whit heavy winds, and the purpose of this thesis was to verify this statement. Data was collected from first- and second thinnings at Boxholms Skogar AB, Östergötland. In first thinnings data was collected from both spruce and pine stands and second thinnings only data from spruce stands was collected.

Värdering av kvävet i organiska gödselmedel :

Within agriculture it is important to optimize the use of nitrogen, not only from an economic point of view but also to minimize damage to the environment. This can sometimes be difficult, especially within organic farming. Organic farmers are using increasing amounts of waste products from the food industry to cover their nitrogen needs. These different organic fertilizers release nitrogen at different rates and under specific soil conditions. Because of this, it would be beneficial to have a standardized method to measure the amount and rate of nitrogen mineralization from organic fertilizers. This project contributes to this knowledge by investigating whether relatively simple and repeatable laboratory methods can be used to estimate the nitrogen fertilizer value of organic fertilizers.

Enkätundersökning bland svenska veterinärer angående behandling av klinisk mastit hos mjölkkor

En webbaserad enkät kring behandling av klinisk mastit hos mjölkkor skickades till 741 svenska veterinärer som enligt Jordbruksverket rapporterat att man under 2012 behandlat fall av mastit. Studien gjordes med syftet att ta reda på hur klinisk mastit behandlas i Sverige 2013. Antalet svarande som inkluderades i studien var 267. Det innebar en svarsfrekvens på 36 %. Enkäten bestod delvis av allmänna frågor kring behandling samt tre fallfrågor.

Silver i luktfria kläder - en stinkande lösning

Genom ökad användning på träningskläder har antibakteriella behandlingar blivit allt vanligare för den allmänna konsumenten. Dessa plagg marknadsförs ofta som antibakte-riella eller luktfria, men det som inte framgår är vilka ämnen som används för att uppnå den luktfria effekten. Vanligt förekommande är de behandlingar som är baserade på oorganiska silversalter. Medan leverantörerna av dessa produkter argumenterar för, argumenterar många miljövetare och forskare på bakterier emot antibakteriella produk-ter då dessa kan ha en skadlig inverkan på vår miljö och hälsa. Rapporten syftar till att ge större kunskap kring miljö- och hälsopåverkan vid användandet av textiler behandlade med silversalter samt till att analysera konsumenters inställning till antibakteriella kläder samt deras användar- och tvättvanor för träningskläder.

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