Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Fetman i Sverige har under de senaste två decennierna ökat kraftigt. Till följd av detta är hälsokostnaderna relaterat till fetma ansenliga och förväntas öka ytterligare om ingenting görs åt problemet. I denna studie studeras prevention och gastric bypass som alternativa behandlingsmetoder mot fetma sett ur ett samhällsekonomiskt perspektiv. Tre hypotetiska kohorter utvecklas; status quo, effektiv prevention i ungdomsåren samt individer som utvecklar svår fetma opereras med gastric bypass. Dessa kohorter följs under åldern 20-64 år där kostnader och effekter observeras med hjälp av en beslutsträdsmodell.

This report includes common helminths, symtoms, routes of infection, diagnostic laboratory techniques and prophylactic methods to prevent infection. We have pointed out where there is a risk of zoonotic transmission and how to avoid contamination and infection. We have restricted the number of species to the most common helminths. Some of these helminths do not naturally occur in Sweden (yet) but are nevertheless of current interest when we travel across borders with our horses, dogs and cats. The diagnostic part of our report tells about different techniques to find an occurance of helminths in samples of faeces, blood, urine or saliva from a horse, dog or cat. We also write about some recommendations on treating parasitic infections and the development of anthelmintic resistant helminths that originates from incorrect use of anthelmintic drugs .

The purpose of this report is to study the relationship between the rescue service fire prevention activities and the number of arson fires in schools. An objective of the project is also to investi-gate the need of developing national methods for fire prevention. The results are based on a questionnaire study among 35 fire departments and further interviews with five of them. The results indicate that there is a relationship between the rescue service fire prevention activities and the number of arson fires in schools. The relationship is positive in the sense that arson fires in schools decrease with increasing prevention activities.

Lactobacillus reuteri has been used as probiotics in piglet and chicken feedings as well as in different diary products for humans. In this EEF-work I have shown that L. reuteri can be an alternative for treatment of vaginitis in laboratory rats used for breeding. In a breeding stock with 3000 animals some animals had a vaginal infection with ??haemolytic streptococci group G.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Folkhälsovetenskapligt program.

AbstractBackground: After a while the usage of urinary catheters leads to UTI which for the patient means inconvenience, suffering and pain. Aim: The aim of this study was to illuminate how to prevent UTI from patients with indwelling catheter. Method: This study was a literature review in which the articles have been searched for in PubMed, Cinahl and ELIN@Kristianstad. Results: It is important for the nurse to clean the periutheral area before inserting the catheter, and has the knowledge of different cathetermaterials. Silver alloy-coated catheters reduces the rate of UTI.

Bovine Respiratory Syncytial Virus (BRSV) is a common cause of respiratory disease among young cattle. The virus causes severe losses; the herd mortality rate can sometimes be as high as 20 %. In this study the avidity (i.e the antigen binding force) of BRSV specific antibodies was measured to see if there was a difference between antibodies produced during an acute phase of infection and antibodies produced by earlier infected animals. A commercially available ELISA-testkit against BRSV-specific antibodies was used and an incubation step with 6M urea was added. The effect of the urea is that it breaks the weak bonds between antibodies and antigen while the stronger bonds remain intact. Four different groups of animals were included in this study; seven calves that were naturally infected, three acutely infected calves with known time of infection, five cows that were seropositive during several years and four experimentally infected calves that had been a part of a vaccine trial. The results of this study showed that antibodies produced during the acute phase of an infection had a low avidity and that the avidity increased with time after infection.

Folkhälsovetenskapligt program.

Every year begomovirus infections cause severe constraints to the tomato production in Nicaragua. Extensive yield losses are followed by large economical losses, both for the country and the single farmer. During recent years the use of begomovirus resistant or tolerant tomato cultivars has increased in Nicaragua as an effort to improve yields. Still, begomovirus infection is a large threat to the tomato production. Therefore, ongoing work in Nicaragua is focusing on finding new, better, begomovirus tolerant or resistant tomato cultivars.

Background Nightingale attention that hygiene was an important task to prevent health- related infection. Today there are guidelines how hand hygiene should be followed, to prevent health- related infection. Good knowledge and education in hand hygiene and its consequences if it?s not followed is of great importance. Hand hygiene should be performed before and after the clean and the unclean work with patients and materials.

Introduction Postoperative wound infections leads to increased patient suffering and cost society money because it leads to longer hospital stays. Previous research shows that patient related risk factors such as smoking, diabetes, Chronic Obstructive Pulmonary Disease (COPD), advanced age (>65 years) a low preoperative hemoglobin level (<140g/L) and a high BMI (> 30) increases the risk of suffering a postoperative wound infection. Aim To investigate whether patients that underwent surgery with Coronary artery Bypass Graft (CABG) in a university hospital in central Sweden in 2013 and had any of these risk factors more often suffer from post-operative wound infection.MethodThe study has a descriptive design and is based on an ongoing infection registration area of operations. The study included 148 patients out of 255 (58%) undergoing CABG in 2013. Possible risk factors were collected through medical record review.Results There was no significant correlation between surgical wound infection and patient-related risk factors such as smoking (not quit smoking four weeks before surgery), diabetes, COPD, advanced age (>65 years), low preoperative hemoglobin (<140g/L) or a high BMI (>30) and suffered a postoperative wound infection.Conclusion More research and a larger sample is needed in the field to produce a reliable result could be presented..

Syftet med denna litteraturstudie är att granska vetenskaplig litteratur med avsikt att undersöka vilka riskfaktorer som leder till utbrändhet bland sjuksköterskor samt hur dessa kan förebyggas..