Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.

Milk allergens were digested by allowing them to flow through a chromatography column, where pepsin was conjugated to the stationary phase of the column. The allergen fragments were then characterized both biochemically, by using SDS-PAGE and gel permeation chromatography, and immunologically, by examining their reactivity to IgE and monoclonal antibodies..

The purpose of this project is to analyze and study the absorption of the substances ivermectin, spinosad and barettin to hydrophobic and hydrophilic surfaces, and to analyze and study if the barnacle larva are effected when they try to settle on the treated surface.Incubation tests with barnacle larva in Petri dishes were performed as well as absorption tests on hydrophobic and hydrophilic surfaces which were tested with ellipsometry. To determine the surface thickness.The barnacles were placed in hydrophobic as well as hydrophilic Petri dishes that had been incubated with ivermectin, spinosad or barettin. After four to six days the larva was counted to analyze the settling. From the results conclusions could be drawn about the adsorption abilities of the substances to the different surfaces.The barnacles were placed in hydrophobic as well as hydrophilic Petri dishes that had been incubated with ivermectin, spinosad or barettin. After four to six days the larva was counted to analyze the settling.

Honey is a product that may contain pesticide residues due to contamination from bees pollinating various plants as well as elimination of vermin inside the hive. Different methods are needed for analysis of pesticides, since the term includes a wide range of different substances. National Food Administration lacks a validated method for determination and quantification of pesticides in honey using liquid chromatography tandem mass spectrometry, LC-MS/MS. The technique is especially important in analysis of polar and non-volatile pesticides, where gas chromatography is not functioning optimally. After investigating what pesticides could be found in honey produced in Sweden, a number of four pesticides suitable for analysis with LC-MS/MS was compiled; boscalid, impidacloprid, tau-fluvalinate and thiacloprid.

A computer model based on minimization of the free energy, capable to predict contact angles and spreading transitions between Wenzel and Cassie mode for drops placed on surfaces with different topography were implemented in matlab. Simulations were compared with experiments documented in the literature. These showed that reported transitions between Cassie and Wenzel mode can be explained by minimization of the free energy. In this report, a study on the possibility of constructing water repellent paper surfaces with a combination of treatment with octadecyltrichlorosilane and topography changes, is included..

It is important to know what is normal in the wound healing process in order to determine what happens when a wound does not heal as expected. If the amount of bacteria in the wound gets too large the body will not be able to defend itself and it becomes an infection. Colonization and infection are common elements in wound management and it is often the veterinary nurse role to deal with wounds in a proper manner. Antibiotics are drugs that either kill or inhibit the multiplication of bacteria or fungi, and have been in veterinary medicine for approximately 60 years. Very few new antibiotics are introduced and increased resistance is a major problem that requires everyone to take responsibility to reduce the use of it.

ABSTRACTMany wind turbines have problems with icing and there is still no effective method to de-ice without stoppage of production and power loss. Here I therefore consider other methods than those available today, and particularly, I consider how these methods could be applied to wind turbines of the company Oxel. A surface where the water droplets do not attach might be the basis for a useful method. If no water attaches, no ice could form. To accomplish this a hydrophobic surface is nessesary, for example wax.

This paper aims to investigate the embalming process and the Balanites aegyptiaca from two pottery originated in ancient Egypt. The two objects were from Medelhavsmuseet in Stockholm. Samples were collected and analyzed with Fourier transform infrared spectrometry (FTIR) and Gas chromatography/mass spectrometry to identify which components the samples contained. The results show complex mixtures mainly consisting of resin origin from Pinaceae and the vegetable oils from the Balanites aegyptiaca..

Today's technology offers a variety of opportunities to interact with the interactive systems. Human-Computer Interaction in the last decade is the area of research that has contributed the most to this development. But how is this interaction perceived? The user experience is something that is formed by the interaction between human and the computer interactive systems. The objective of this thesis is to investigate how the user experience differs depending on how one interacts with a game.

Viewer interaction is a common part of live broadcasts on the web and enables production and viewer to communicate with each other. The possibilities in viewer interaction make it easier for productions to create viewer engagement by involving them in the live broadcast through chats and social media. In creating a bond between production and viewer the interaction must be carefully managed to suit both the shows and the viewer?s needs.  A qualitative study has been used to analyze the viewer interaction in two Swedish live broadcasts on the web.

Mobile technology is developing quickly and becomming a part of daily life and communication. Bluetooth Low Energy is presented as a new way of mobile interaction. However there are not enough studies in this field on the interaction between mobile devices and the physical world. In order to understand user needs, and to encourage users to interact, we simulated an interaction process through designing and testing a prototype in a specific indoor environment. Prototype effectiveness and main characteristics for future mobile application was evaluated through a qualitative user study with 20 participants who interacted with our prototype..

This field study examined how elderly and cognitively disabled people used and experienced a social companion robot. The following pages explores the questions: Which are the physical, social and affective qualities during the interaction? The aim was to through observations see how qualities of interaction could activate different forms of behavior. The results show that motion, sound and the eyes together created communicative and emotional changes for users who felt joy and were willing to share the activity with others. The robot stimulated to some extent users to create their own imaginative experiences but often failed to involve user or group for a long time and was also considered too large and heavy to handle..

In this bachelor thesis we have studied how interaction designers describe their profession. The research material is based on nine interviews with interaction designers. Questions cover areas such as interaction design tasks, what abilities they consider important, how they describe their relationship to the users, and cooperation with colleagues. We have analyzed the results using key terms such as habitus, field, and capital, as described by sociologist Pierre Bourdieu. The interviewees express humanistic views, and ask for more cooperation between interaction designers, as well as other people within the field of IT production.

The purpose of this study is to find out how teachers in preschool interact with children.By unstructured observations of teachers in preschool I intend to find out how they interact with children. Is it possible to see that the operation is built on guided interaction speciality?Positive interaction between adults and children is of importance to a good childhood and a child?s learning. I have asked myself how teachers interact with children in preschool. There are different ways of interaction.

Starch is a naturally occurring substance in most plants and is used as energy storage. It is a component that mainly consists of amylose and amylopectin. Together they build a complex pattern which gives starch its specific properties. The proportion of amylose and amylopectin varies in different plants but generally starch consists of 25 % amylose and 75 % amylopectin. There have been discoveries of barley varieties containing 100 % amylopectin called ?waxy starches? or ?waxy barley? as well as varieties that have a high content of amylose, around 70 % and these are called ?amylotypes?.