Biokemisk och immunologisk karaktärisering av pepsin-spjälkade mjölkallergener
Food allergyPepsinDigestionCow milkIgEImmunoCAPFödoämnesallergiPepsinMatspjälkningKomjölkIgEImmunoCAP
Milk allergens were digested by allowing them to flow through a chromatography column, where pepsin was conjugated to the stationary phase of the column. The allergen fragments were then characterized both biochemically, by using SDS-PAGE and gel permeation chromatography, and immunologically, by examining their reactivity to IgE and monoclonal antibodies.