Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .

Together with NCC and Sweco we measured the air-flow on a semi-detached house in Jönköping. When a pressure measurement is made, the house gets exposed to over- and under pressure. This is done in order to examine the houses climate shell. The climate shell of the semi-detached house we tested exceeded the required air thickness in BBR-2002, hence, measures have to be taken.This report also displays the importance of building air thick houses and what consequences a non air-thick house can have for the ones residing the house. A discussion of different measures is on for the addressed problems with the flow-measurement and the alternative construction-solutions.Furthermore we have described in this report of how to account for the performance of the air pressure, what kind of equipment we used and how we came up with the results.

This literature review summarizes available data on infection with the parasitic roundworm, Ascaridia galli, as a cause of disease in poultry, including the consequences of a conversion to alternative production systems. Ascaridia galli has a direct life cycle with resistant eggs that survive and are easily spread in the environment, especially in systems with bedding materials where the faecal contact is high. The infection is usually subclinical but depending on the infection dose, the symptoms range from weight loss and diarrhea to mortality. Factors such as the feed composition and genetic variation among different hybrids clearly affect the establishment rate of the infection. Ascaridia galli will probably become an increasing problem, with a shift from the traditional battery cages to alternative systems where the risk of re-infection is high.

Due to higher standards of living around the world, greater industries and larger infrastructures causes an increase of the global energy consumption. This harvest of energy puts a great stress on the global environment. With this development, it becomes increasingly important to utilize energy resources in the best possible way.Fans are components that are often used in the industry and in households. A common problem is that the fan is oversized. An oversized fan causes an excessive flow, which has to be adjusted in order to achieve the correct flow.

Earlier investigations showed that damages on timber from feeding rolls could be within the lower classes of damage according to VMRs classes. Knowledge about differences in productivity between kind types and aggressive types of feeding rolls is necessary because it should influence the question about damages on timber caused by different feeding rolls. In this study the production and timber damage in a harvesting head were evaluated at three levels of pressure (8, 10 and 14 MPa) on the feeding rolls, and with three different types of rolls. One type, the rubber cushioned steel plate roller had 10 mm studs and rubber damping, one ?middle? type made of solid steel with 14 mm studs, and one aggressive type, also made of solid steel with 18 mm studs.

Studien visar att en positiv onormal avkastning uppstår i och med att kungörelsen av ett noteringsplatsskifte offentliggörs. Den positiva onormala avkastningen återgår från och med det fysiska skiftet till OMX från de mindre noteringsplatserna. En temporär värdeutveckling som stöds av Price pressure hypotesen och som förklaras av handelsvolymförändringar i aktien..

The study was carried out at the Kungsängen Research Centre, Department of Animal Nutrition and Management, Swedish University of Agriculture Sciences, Uppsala. The technical development in dairy production has resulted in a housing system where feeding and milking is done automatically without human contact, a so called automatic milking system. The motives for utilising an automatic milking system, is to increase the animal welfare and to decrease the need for manual labour. However, the system does involve a change for the animals, among the effects is the deprival or the diminishment of a chance for synchronous behaviour and the animals have to agree among themselves in which order they use the feeding stations and the milking unit itself. How and if this affects the animals well-being and production is yet to be solved. A possible way to form an opinion on this, is to study the animals ranking order along with hormone profiles and production. The purpose of this work was to study the effect of low- vs.

Staphylococcus aureus is the most clinically important Staphylococcus species and is associated with high mortality in patients with positive blood cultures. S. aureus bacteria may cause a variety of disease manifestations ranging from minor skin infections to life-threatening conditions such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS) and sepsis. This microorganism belonging to the gram positive cocci may also be part of the normal flora. In Sweden, penicillinase-stable penicillins are the primary alternatives to treat S.

Syfte: Syftet var att granska det vetenskapliga underlaget och analysera om det fanns effekter av bl?b?rsintag p? plasmaglukos (P-glukos), systoliskt blodtryck (SBP) och diastoliskt blodtryck (DBP) hos vuxna med normala niv?er av P-glukos, SBP och DBP. Metod: En systematisk litteraturs?kning gjordes i databaserna PubMed och Scopus d?r randomiserade kontrollerade studier efters?ktes. Inkluderad population var individer med normala P-glukosniv?er och blodtrycksniv?er samt utan funktionsneds?ttning eller diagnostiserad sjukdom. Interventionen var f?rska, alternativt frysta bl?b?r eller frystorkat bl?b?rspulver.

The purpose of the work is to analyze and balance an assembly line at Fläkt Woods in Jönköping. The work is going to answer the questions about today?s throughput time, how much waste that occurs, number of staff at the assembly line, how the supply of material works and possible improvements that can be made at the work stations. The work is also going to give a proposal of improve-ments that can be made.The methods used to get the result include observations and measurements, con-versations with technicians and operators and also data from Fläkt Woods? data-base.Large variations in throughput time are measured during the measure period, from 3 hours up to 5 hours and 35 minutes.

In the western world, strokes are the third most common cause of death, hitting mainly the elderly population. Smoking, High blood pressure, diabetes and overweight are known risk factors for causing strokes. Previous studies show that an increased level of particulate matter, PM, is also related to an increased number of strokes. The population in this study consists of patients over 65 years old who live in Malmö. Levels of particulate matter were measured from October 2004 to April 2005 at a central outdoor monitoring site in Malmö.

This paper analyses the character judge Holden in Cormac McCarthys novel Blood Meridian through an ethical perspective. The author uses several methods to analyze the character ethically, such as the western-genre, McCarthys authorship, evil as an ethical term and Friedrich Nietzsche's theory the Übermensch. The author then uses a selective group of scenes and chapters from the novel to highlighting the distinctive ethical characteristic of judge Holden. The author finds judge Holden to be the very principle of violence and blood shedding, defending and distributing these principles in his words and his actions throughout the novel. Triumphantly proclaiming his victory, judge Holden embodies the principle that whoever has the power to perform any action has then the moral right to do so.

This literature review summarizes available data on infection with the parasitic roundworm, Ascaridia galli, as a cause of disease in poultry, including the consequences of a conversion to alternative production systems. Ascaridia galli has a direct life cycle with resistant eggs that survive and are easily spread in the environment, especially in systems with bedding materials where the faecal contact is high. The infection is usually subclinical but depending on the infection dose, the symptoms range from weight loss and diarrhea to mortality. Factors such as the feed composition and genetic variation among different hybrids clearly affect the establishment rate of the infection. Ascaridia galli will probably become an increasing problem, with a shift from the traditional battery cages to alternative systems where the risk of re-infection is high.

The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

Staphylococcus aureus is the most clinically important Staphylococcus species and is associated with high mortality in patients with positive blood cultures. S. aureus bacteria may cause a variety of disease manifestations ranging from minor skin infections to life-threatening conditions such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS) and sepsis. This microorganism belonging to the gram positive cocci may also be part of the normal flora. In Sweden, penicillinase-stable penicillins are the primary alternatives to treat S.