Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

This study is a part of a Swedish research and development project that is about heat-treated wood and is led by the Swedish University of Agricultural Sciences and Luleå University of Technology. Products that are heat-treated already exists on the market, foremost the Finnish brand ThermoWood®, bur the project group want to find out if it would be possible to start a Swedish production with somewhat different applications than what is common today. The heat-treated wood has many attributes that have changed in the heating process. It becomes more biologically durable, it gains stability in form and dimensions and it becomes darker and looses several unwanted extractives such as resin. Disadvantages that can be mentioned are that the wood becomes fragile and the strength properties changes. My main purpose with this study is to look into customer involvement in the early phase of product development. I also want to find out if the customers I interview think that the product is worth working with, which means start a Swedish production.

In cooperate with Asko Cylinda we have developed a new heat system too short the processtime and reduce the energy consume in a dishwasher and still have it too be a class AAA.The environment and quality is important for the company too still be the number one onthe market, If they want too keep that position the product developed of new innovations willbe very important. The systems we have developed are more effective and reduce energy uptoo 25 %. The process time is reduced too less than two hours, from the today two andhalf-hour. The solutions are too heat up the rinse water and the dishwater at the same time,with a new heating system and it make are solutions unique and effective. Its good for thedish and the environment.

At Forsmark nuclear power plant the rather old-fashioned software COPTA is used in containment safety analysis. There exists a desire within the organisation to introduce a more modern software with ability to more detailed modeling and increased usability.The goal of this thesis was to evaluate the software APROS in containment safety analysis. APROS models describing one of the containments at Forsmark NPP have been developed. Two simulations of typical containment incidents, one of them a large pipe break, have been made where containment safety parameters such as pressure and temperature are studied. Results are analyzed and verified against results from corresponding COPTA models.

Works on transparent paper, also commonly known as tracing paper, have been described as ephemeral or temporary working documents and they usually number large quantities in archival and museum collections. They have therefore previously been considered of lower priority and can be found in poor condition due to a lack of treatment or suitable storage prospects. In addition, transparent paper can be very sensitive to moisture and reacts by deformation or loss of transparency. Due to the volume of these works and the necessity of treatment, there is a call for simple and efficient conservation methods. A common solution in archives for the treatment of water-sensitive works on paper has been the use of heat-activated tissues as they can be applied without the introduction of solvents. There is little published about the use of heat-activated tissues on transparent paper and is mentioned only briefly in existing literature.

The purpose of this thesis has been to determine experimentally how good a peltierelement is to convert waste heat from wood burning. Three different peltier elementswere used in a comparison study. The comparison was mainly done to know howmuch power could be obtained from each element. Even, a circuit with a step-upfunction has been created to give a higher output voltage than the peltier elementitself. The result of the experiments was a low output power, the highest valuereached 2.18W.

I want to look at the possibilities to start selling hot water from a small district heating plant with bioenergy. My reason for doing this project is to look at a new business opportunity for our farm. Our company have less work during the winter time compared to the spring and this project will give us more work during the winter. I also want to take as much material as possible for heating from our farm in order to increase the value of the products from the farm. The project is in a village near us, where we should support a school, a churh, a home for the aged, a kindergarten and a parish hall with heat. In my work I have compared two different alternatives with each other. The first alternative is to put a central heater in one of the buildings, the home for the aged, in which we will burn oat.

Climate change is the modern society?s common issue. The developed countries areconsuming more than what is sustainable, and the climate change due to the use offossil fuels can be seen clearly. EU has specified goals, in order to stop furtherdamage, which demand that every member state have to reduce its energyconsumption by 20 % before 2020 and 50 % before 2050, relative to 1995.The Swedish government uses a number of instruments to encourage reduction in theusage of energy, but every individual has to contribute to reach the goals.With increasing energy price the cost of living is going up. A lot of people are lookingfor ways to save energy and not be affected as much by the increasing price.Investments for insulation, replacement of windows and replacing old heating systemfor a new efficient heat pump is getting more and more common.This thesis is about investigating the possibilities in becoming independent from buyingenergy for households.

This report deals with the theory and empirical evidence of how knowledge generated within a project is to be captured and shared with other projects and the surrounding organization, after the end of a project. A number of authors have highlighted knowledge management within a company as a key component in building a unique competitive advantage and knowledge should therefore be considered a very important resource within companies. The difficulties of ensuring that knowledge generated within a project can benefit other projects are many. The nature of the project form has been highlighted as one of the biggest problems, due to the fact that budget and time requirements often restrict the propensity to work with knowledge transfer. Other difficulties that this report highlights are in particular the lack of a knowledge sharing culture within the organizations, as well as human cognitive and psychological limitations and lack of tools design for knowledge transfer.

The aims of this project were to describe the circumstances around dogs being left alone in cars, to investigate owners? knowledge of the risks associated with leaving dogs in cars and the relevant legislation and, finally, to study how changes in the air temperature and air composition in the car affect the dog. The project consisted of a survey of the literature, a questionnaire part and an experimental part. An important conclusion is the importance of the level of knowledge of owners, since there are several ways in which an owner can prevent their dog from heat stroke and, in the case of an eventual accident, their action can have direct consequences in minimizing the long-term damage to the dog and even whether or not it survives. Fewer than half of the owners in the study knew that for a dog suffering heat stroke, the most important thing is to bring the body temperature down. Only a third of dog owners knew that if a dog is left in a warm car and showing signs of heat stress, then the police should be contacted and the dog quickly helped out of the car.

This degree project has been carried out in cooperation with Enertech AB in Ljungby and is pilot study to automating all current manual soldering. The soldering process accounts for a large part of the processing cost in the production of a heat pump. The process is also a quality risk and has a major impact on the lifetime of the final product.The Main task of this project has been to evaluate automation of the soldering process, to develop automation solutions and a principle layout, to draw the design changes required for the automation and to calculate the supposed solution.This pilot study has resulted in a suggested automation solution using laser as a heat source for soldering. Calculations of the solution show that a automating a laser soldering cell is very profitable..

AbstractWith increasing population and increasing demands for comfort and thus an increase in energy demand, it is necessary to examine various energy-efficiency measures. This work deals with the ability to control the flow through the heating system to achieve the desired comfort with as little energy as possibleA heating system contains of different parts, roughly speaking, you can divide it to three main components: Heat source, Distribution and Heat emitters. All components are working together to bring heat to the building. What is examined in this report are different ways of adjusting the flow and supply temperature and how it affects comfort and energy use in a building.At today ?s date the conventional method is to use a high flow through the heating system, which affects pressure drop and cooling of the fluid.

This thesis examines a possible control strategy to minimize flow fluctuations in Uppsala?s district heating system. High variations in flow rate may lead to complications caused by hydraulic constraints in the district heating system. These complications, such as inadequate pressure and temperature levels, increase the risk of heat delivery failures. It is therefore important to avoid such situations both for the producer and for the customers.Typically, the forward temperature is controlled by using a rough correlation with respect to the outdoor temperature.

Companies that sell digital products such as software and movies transfer large amounts of data to its customers. In order to send this amount of data using FTP or HTTP and still maintain a high speed transfer rate it is required that the company have a large number of servers and a huge bandwidth. Servers and bandwidth costs money which means they have great costs on the distribution of their products. In order to reduce distribution costs, P2P technology can be used and the most powerful technology to transmit popular files is called BitTorrent.BitTorrent requires users to share their bandwidth. Nowadays, BitTorrent is frequently used to transfer products that are free of charge; one example is the Linux distribution Ubuntu.

Following the accident at the nuclear power plant in Chernobyl in 1986, great volumes of137Cs were distributed over large parts of the former Soviet Union and Europe. Over Scotland,radiocaesium was deposited as wet deposition. Due to the relatively long physical half-life of30 years, there are still measurable activity concentrations in the natural environment. Thisstudy was made on two organic soils, Knockandy Hill and Rora Moss, situated in the easternpart of the central highlands of Scotland. At these two sites, studies on migration and transferbetween soil and plants were conducted.