The purpose of this study was to develop a reliable molecular-genetic method to determine the different species of helicobacter in dogs. The study is part of a larger project to map the prevalence of Helicobacter spp in healthy and sick dogs in Sweden, and to determine the possible connection of Helicobacter spp infection with gastrointestinal diseases in dogs. Several published studies have reported on the prevalence of Helicobacter spp in dogs. The problem is that three of the most common species are so alike that a 16SrRNA-PCR with sequencing is not able to differentiate between them. In this study, DNA has been purified from samples and then a Multiplex PCR has been performed. Multiplex PCRs use multiple primers in one single PCR.

Sahlgrenska Academyat University of GothenburgDepartment of Internal Medicin and Clinical NutritionAbstractTitle: Does supplementation of omega-3 during pregnancy affectprevalence of allergy in infants and children?Author: Peter Eliasson, Anna RahmSupervisor: Lena HulthénExaminer: Anna WinkvistProgramme: Dietician study programme, 180/240 ECTSType of paper: Examination paper, 15 ECTSDate: 2012-04-12BackgroundEpidemiologic research has shown an association between a high intake of fish and allergy.The underlying theory is that allergy develops already at a prenatal stage and food intakeduring pregnancy has been shown to be significant. Conducting research on allergy isproblematic since it has genetic, as well as enviromental, determinants.ObjectiveThis thesis attempts to conclude and evaluate research on a connection betweensupplementation of omega-3 during pregnancy and allergic outcomes in infants and children.Search strategyThe literature search was conducted in the databases PubMed and Scopus. Among thekeywords chosen were omega 3, supplement, pregnancy and allergy.Selection criteriaHuman RCT, that measured allergy, where the intervention consisted of supplementation withomega-3 for at least ten weeks during pregnancy and ceased at birth.Data collection and analysisThree articles were chosen for this thesis. The articles were examined using templates fromSBU and the quality of evidence was evaluated according to GRADE.Main resultsNone of the articles showed a protective effect on allergic outcome but in one of the studiesthere was significant reduction in eggallergy and another showed significant lower occurrenceof severe eczema.ConclusionsSupplementation with omega-3 during pregnancy does not occur to have an protective effecton allergic outcome in infants and childen but the evidence for this is considered to bemoderate.

Sammanfattning I september 2008 fångades 311 juveniler (unga individer) av arten sill (Clupea harengus) in vid fyra olika områden på den svenska västkusten; Råssö, Hunnebo, Askerö- och Gullmarfjorden. Genetiska, kemiska och morfologiska data (längd och vikt) samlades in i syfte att bestämma vilka populationer de härstammade ifrån. Fångster av sill är så gott som alltid av blandat ursprung eftersom individer bara samlas i de populationer de stammar ifrån vid den tid på året då det är dags för parning (lek). I den här uppsatsen klassificerar jag juvenilerna till tre regioner i vilka populationerna ingår, genom att jämföra de data som samlats in från juvenilerna med motsvarande data i en stor databas över populationer i Nordsjön, Skagerrak, Kattegat och Östersjön samt med ytterligare en population, Risør, som inte ingår i denna databas. Data om populationerna kommer från samples och de sanna populationsparametrarna (dvs.

Ataxi är då hunden har svårt att koordinera sina rörelser och orsakas av att nervsystemet inte fungerar som det ska och degenereras. Degeneration av cerebellum och då specifikt purkinjecellerna och granulacellerna ger en form av ataxi kallat cerebellär ataxi. Denna typ av ataxi uppvisas genom att hunden (Canis lupus familiaris) står bredbent, har muskelkramper, obalanserad gång och ögonrullningar. Den genetiska bakgrunden till cerebellär ataxi hos hund finns beskriven hos flera raser, däribland beagle, finsk stövare, old english sheepdog, gordonsetter och coton de tuléar. Hos alla dessa raser nedärvs sjukdomen autosomalt recessivt och de orsakande mutationerna ger olika dysfunktioner inom nervcellerna som gör att de inte kan fungera som de ska och degenereras.

Med genteknik är det möjligt att ändra gensammansättningen i våra livsmedel och applikationen har väckt stort intresse, inte minst bland allmänheten. Genmodifierade (GM) livsmedel har varit föremål för diskussion sedan 1970-talet. Syftet med denna uppsats är att studera olika uppfattningar och representationer om genmodifierade livsmedel. Enligt teorin om sociala representationer har representationer dubbla funktioner. Den ena är att konventionalisera objekt och den andra innebär att representationerna intar en förutbestämd form.

Multiple Congenital Ocular Anomalities (MCOA) is a genetic disease that affects primarily Silver coloured horses of breeds such as the Rocky and Kentucky mountain horses. In these breeds the Silver dapple colour is very popular leading to an increase of affected horses. The major feature of the disease is ocular cysts of variable size. Large cysts also lead to a variety of secondary syndromes, for example retinal detachment. In previous studies the locus for MCOA has been mapped to an interval of 420 kb on equine chromosome six.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

Due to exploitation of the world?s rivers, the upstream migration of anadromous species is frequently delayed or even prevented. To mitigate these problems and allow fish to migrate past obstacles, structures such as fish ladders have been developed. However, recent studies show that many of the present fish passage facilities are deficient. Monitoring and evaluation of passage facilities is therefore crucial to enable necessary adjustments.

The Swedish Red Polled breed is threatened by extinction and there are only around 1100 animals left. The breed was popular in the early twentieth century, but since then the numbers have steadily decreased. The overall objective of this study was to find out whether there may be economic incentives for preserving the breed. The milk quality properties are of major importance in the production of dairy products. Therefore we would like to establish if the Swedish Red Polled breed carries favourable alleles at loci of relevance for milk quality, to potentially make the breed more interesting for milk production.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

Hybridasp (Populus x wettsteinii) är en snabbväxande hybrid mellan europeisk asp (Populus tremula L.) och nordamerikansk asp (Populus tremuloides Mich.). Det finns svenskt hybridaspmaterial framtaget för odling i Götaland men inte för övriga delar av Sverige. För odling av hybridasp i övriga delar av Sverige rekommenderas ofta finländskt odlingsmaterial. Hybridasp lämpar sig väl som biobränsle tack vare dess höga tillväxt och då efterfrågan på biobränslen förväntas öka är det viktigt att ta fram ett hybridaspmaterial som har högre härdighet och tillväxt än de hybridaspkloner som finns på marknaden idag. Målet med denna studie var att göra ett urval av lämpliga hybridaspkloner för odling i södra och mellersta Norrland utifrån försök med nyframtagna korsningar.

The aim of this study was to investigate whether a dietary change, physical activity or a combination of both gave the best result when treating obesity, concerning weight loss and BMI. Excessive weight and obesity are today a big health-issue and it is estimated that 10 % of the men and 12 % of the women in Sweden have a BMI over 30, i.e. are suffering from obesity. The corresponding number for children is 3 %. No one can say for sure what causes obesity but there are many theories.

The fertility of dairy cows is of great importance in order to maintain high production. The decline in fertility of dairy cows the latest decades can have several explanations. The large emphasis on high yielding cows and the negative genetic correlation between milk production and fertility traits could be one of the main factors for impaired fertility. To find heats and inseminate the cow at the right time is crucial to receive high conception rate. Delaval has, in corporation with FOSS, developed Herd Navigator which is a management program that measure four biological parameters in the milk; progesterone, betahydroxybutyrate, lactate dehydrogenase and urea.

A nonsense mutation in the DMRT3 gene has been shown to have a large impact on pattern of locomotion in horses. Horses that can perform several other gaits in addition to the normally occurring gaits, walk, trot and canter, are often hetero (CA)- or homozygous (AA) for this nonsense mutation. Horses that only can perform walk, trot and canter are often homozygous for the wild-type gene (CC). For example the Icelandic Horse is a gaited breed. Five-gaited Icelandic horses can perform both flying pace and tölt, except for the normally occurring gaits, walk, trot and canter, and are often homozygous for this nonsense mutation (AA).